Ultrastructure of Organohalide-Respiring Dehalococcoidia Revealed by Cryo-Electron Tomography

Sexton, Danielle L.; Chen, Gao; Murdoch, Fadime Kara; Hashimi, Ameena; Löffler, Frank E.; Tocheva, Elitza I.

doi:10.1128/aem.01906-21

Title: Ultrastructure of Organohalide-Respiring Dehalococcoidia Revealed by Cryo-Electron Tomography

Journal Article · 25 January 2022 · Applied and Environmental Microbiology

DOI: https://doi.org/10.1128/aem.01906-21 · OSTI ID:1843693

Sexton, Danielle L. ^[1]; Chen, Gao ^[2]; Murdoch, Fadime Kara ^[3]; Hashimi, Ameena ^[1];

^[4];

^[1]

Univ. of British Columbia, Vancouver, BC (Canada)
Univ. of Tennessee, Knoxville, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States); Battelle Memorial Inst., Columbus, OH (United States)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Dehalococcoides mccartyi (Dhc) and Dehalogenimonas spp. (Dhgm) are members of the class Dehalococcoidia, phylum Chloroflexi, characterized by streamlined genomes and a strict requirement for organohalogens as electron acceptors. Here, we used cryo-electron tomography to reveal morphological and ultrastructural features of Dhc strain BAV1 and “Candidatus Dehalogenimonas etheniformans” strain GP cells at unprecedented resolution. Dhc cells were irregularly shaped discs (890 ± 110 nm long, 630 ± 110 nm wide, and 130 ± 15 nm thick) with curved and straight sides that intersected at acute angles, whereas Dhgm cells appeared as slightly flattened cocci (760 ± 85 nm). The cell envelopes were composed of a cytoplasmic membrane (CM), a paracrystalline surface layer (S-layer) with hexagonal symmetry and ~22-nm spacing between repeating units, and a layer of unknown composition separating the CM and the S-layer. Cell surface appendages were only detected in Dhc cells, whereas both cell types had bundled cytoskeletal filaments. Repetitive globular structures, ~5 nm in diameter and ~9 nm apart, were observed associated with the outer leaflet of the CM. We hypothesized that those represent organohalide respiration (OHR) complexes and estimated ~30,000 copies per cell. In Dhgm cultures, extracellular lipid vesicles (20 to 110 nm in diameter) decorated with putative OHR complexes but lacking an S-layer were observed. Furthermore, the new findings expand our understanding of the unique cellular ultrastructure and biology of organohalide-respiring Dehalococcoidia.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1843693

Journal Information:: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 2 Vol. 88; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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