Engineered Sorghum Bagasse Enables a Sustainable Biorefinery with p-Hydroxybenzoic Acid-Based Deep Eutectic Solvent

Wang, Yunxuan; Meng, Xianzhi; Tian, Yang; Kim, Kwang Ho; Jia, Linjing; Pu, Yunqiao; Leem, Gyu; Kumar, Deepak; Eudes, Aymerick; Ragauskas, Arthur J.; Yoo, Chang Geun

doi:10.1002/cssc.202101492

Title: Engineered Sorghum Bagasse Enables a Sustainable Biorefinery with p-Hydroxybenzoic Acid-Based Deep Eutectic Solvent

Journal Article · 18 October 2021 · ChemSusChem

DOI: https://doi.org/10.1002/cssc.202101492 · OSTI ID:1841469

Wang, Yunxuan ^[1];

^[2]; Tian, Yang ^[3];

^[4]; Jia, Linjing ^[1];

^[5];

^[6];

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^[3];

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State Univ. of New York (SUNY), Syracuse, NY (United States)
Univ. of Tennessee, Knoxville, TN (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Joint BioEnergy Institute (JBEI), Emeryville, CA (United States)
Korea Inst. of Science and Technology, Seoul (South Korea). Clean Energy Research Center; Univ. of British Columbia, Vancouver, BC (Canada)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation (CBI); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS); Univ. of Tennessee, Knoxville, TN (United States)
The Michael M. Szwarc Polymer Research Inst., Syracuse, NY (United States); State Univ. of New York (SUNY), Syracuse, NY (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation (CBI); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS); Univ. of Tennessee, Knoxville, TN (United States); Univ. of Tennessee, Knoxville, TN (United States). Inst. of Agriculture, Center of Renewable Carbon

Abstract Integrating multidisciplinary research in plant genetic engineering and renewable deep eutectic solvents (DESs) can facilitate a sustainable and economic biorefinery. Herein, we leveraged a plant genetic engineering approach to specifically incorporate C ₆ C ₁ monomers into the lignin structure. By expressing the bacterial ubiC gene in sorghum, p ‐hydroxybenzoic acid (PB)‐rich lignin was incorporated into the plant cell wall while this monomer was completely absent in the lignin of the wild‐type (WT) biomass. A DES was synthesized with choline chloride (ChCl) and PB and applied to the pretreatment of the PB‐rich mutant biomass for a sustainable biorefinery. The release of fermentable sugars was significantly enhanced (∼190 % increase) compared to untreated biomass by the DES pretreatment. In particular, the glucose released from the pretreated mutant biomass was up to 12 % higher than that from the pretreated WT biomass. Lignin was effectively removed from the biomass with the preservation of more than half of the β‐Ο‐4 linkages without condensed aromatic structures. Hydrogenolysis of the fractionated lignin was conducted to demonstrate the potential of phenolic compound production. In addition, a simple hydrothermal treatment could selectively extract PB from the same engineered lignin, showing a possible circular biorefinery. These results suggest that the combination of PB‐based DES and engineered PB‐rich biomass is a promising strategy to achieve a sustainable closed‐loop biorefinery.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC05-00OR22725; CBET2027125; AC02-05CH11231

OSTI ID:: 1841469

Alternate ID(s):: OSTI ID: 1826806; OSTI ID: 1960385

Journal Information:: ChemSusChem, Vol. 14, Issue 23; ISSN 1864-5631

Publisher:: ChemPubSoc EuropeCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
biorefinery
green solvent
lignin
lignin depolymerization
sustainable process

Title: Engineered Sorghum Bagasse Enables a Sustainable Biorefinery with p-Hydroxybenzoic Acid-Based Deep Eutectic Solvent

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