Engineered Sorghum Bagasse Enables a Sustainable Biorefinery with p-Hydroxybenzoic Acid-Based Deep Eutectic Solvent

Wang, Yunxuan; Meng, Xianzhi; Tian, Yang; Kim, Kwang Ho; Jia, Linjing; Pu, Yunqiao; Leem, Gyu; Kumar, Deepak; Eudes, Aymerick; Ragauskas, Arthur J.; Yoo, Chang Geun

doi:10.1002/cssc.202101492

Title: Engineered Sorghum Bagasse Enables a Sustainable Biorefinery with p-Hydroxybenzoic Acid-Based Deep Eutectic Solvent

Journal Article · 17 October 2021 · ChemSusChem

DOI: https://doi.org/10.1002/cssc.202101492 · OSTI ID:1841469

Wang, Yunxuan ^[1];

^[2]; Tian, Yang ^[3];

^[4]; Jia, Linjing ^[1];

^[5];

^[6];

^[1];

^[3];

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^[6]

State Univ. of New York (SUNY), Syracuse, NY (United States)
Univ. of Tennessee, Knoxville, TN (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Joint BioEnergy Institute (JBEI), Emeryville, CA (United States)
Korea Inst. of Science and Technology, Seoul (South Korea). Clean Energy Research Center; Univ. of British Columbia, Vancouver, BC (Canada)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation (CBI); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS); Univ. of Tennessee, Knoxville, TN (United States)
The Michael M. Szwarc Polymer Research Inst., Syracuse, NY (United States); State Univ. of New York (SUNY), Syracuse, NY (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation (CBI); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS); Univ. of Tennessee, Knoxville, TN (United States); Univ. of Tennessee, Knoxville, TN (United States). Inst. of Agriculture, Center of Renewable Carbon

Integrating multidisciplinary research in plant genetic engineering and renewable deep eutectic solvents (DESs) can facilitate a sustainable and economic biorefinery. In this work, we leveraged a plant genetic engineering approach to specifically incorporate C6C1 monomers into the lignin structure. By expressing the bacterial ubiC gene in sorghum, p-hydroxybenzoic acid (PB)-rich lignin was incorporated into the plant cell wall while this monomer was completely absent in the lignin of the wild-type (WT) biomass. A DES was synthesized with choline chloride (ChCl) and PB and applied to the pretreatment of the PB-rich mutant biomass for a sustainable biorefinery. The release of fermentable sugars was significantly enhanced (~190 % increase) compared to untreated biomass by the DES pretreatment. In particular, the glucose released from the pretreated mutant biomass was up to 12 % higher than that from the pretreated WT biomass. Lignin was effectively removed from the biomass with the preservation of more than half of the β-Ο-4 linkages without condensed aromatic structures. Hydrogenolysis of the fractionated lignin was conducted to demonstrate the potential of phenolic compound production. In addition, a simple hydrothermal treatment could selectively extract PB from the same engineered lignin, showing a possible circular biorefinery. These results suggest that the combination of PB-based DES and engineered PB-rich biomass is a promising strategy to achieve a sustainable closed-loop biorefinery.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)

Grant/Contract Number:: AC05-00OR22725; AC02-05CH11231

OSTI ID:: 1841469

Alternate ID(s):: OSTI ID: 1826806; OSTI ID: 1960385

Journal Information:: ChemSusChem, Journal Name: ChemSusChem Journal Issue: 23 Vol. 14; ISSN 1864-5631

Publisher:: ChemPubSoc EuropeCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
biorefinery
green solvent
lignin
lignin depolymerization
sustainable process

Title: Engineered Sorghum Bagasse Enables a Sustainable Biorefinery with p-Hydroxybenzoic Acid-Based Deep Eutectic Solvent

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