Upregulation of CD14 in mesenchymal stromal cells accelerates lipopolysaccharide-induced response and enhances antibacterial properties

Hirakawa, Matthew P.; Tjahjono, Nikki; Light, Yooli K.; Celebi, Aleyna N.; Celebi, Nisa N.; Chintalapudi, Prem; Butler, Kimberly S.; Branda, Steven S.; Krishnakumar, Raga

doi:10.1016/j.isci.2022.103759

Title: Upregulation of CD14 in mesenchymal stromal cells accelerates lipopolysaccharide-induced response and enhances antibacterial properties

Journal Article · 31 January 2022 · iScience

DOI: https://doi.org/10.1016/j.isci.2022.103759 · OSTI ID:1840803

;

; Light, Yooli K.;

;

; Chintalapudi, Prem;

;

Not Available

View Journal Article

Cite

Export

Save

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE Laboratory Directed Research and Development (LDRD) Program; USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: NA0003525

OSTI ID:: 1840803

Journal Information:: iScience, Journal Name: iScience Journal Issue: 2 Vol. 25; ISSN 2589-0042

Publisher:: ElsevierCopyright Statement

Country of Publication:: Netherlands

Language:: English

References (98)

Human mesenchymal stromal cells express CD14 cross-reactive epitopes Pilz, Gregor A.; Braun, Julian; Ulrich, Christine Cytometry Part A, Vol. 79A, Issue 8 https://doi.org/10.1002/cyto.a.21073	journal	July 2011
Antimicrobial effects of mesenchymal stem cells primed by modified LPS on bacterial clearance in sepsis Saeedi, Pardis; Halabian, Raheleh; Fooladi, Abbas Ali Imani Journal of Cellular Physiology, Vol. 234, Issue 4 https://doi.org/10.1002/jcp.27298	journal	September 2018
A Novel Therapeutic Approach Using Mesenchymal Stem Cells to Protect Against Mycobacterium abscessus Kim, Jong-Seok; Cha, Sang-Ho; Kim, Woo Sik Stem Cells, Vol. 34, Issue 7 https://doi.org/10.1002/stem.2353	journal	March 2016
TLR3 or TLR4 Activation Enhances Mesenchymal Stromal Cell-Mediated Treg Induction via Notch Signaling Rashedi, Iran; Gómez-Aristizábal, Alejandro; Wang, Xing-Hua Stem Cells, Vol. 35, Issue 1 https://doi.org/10.1002/stem.2485	journal	September 2016
Antibacterial Effect of Human Mesenchymal Stem Cells Is Mediated in Part from Secretion of the Antimicrobial Peptide LL-37 Krasnodembskaya, Anna; Song, Yuanlin; Fang, Xiaohui Stem Cells, Vol. 28, Issue 12 https://doi.org/10.1002/stem.544	journal	December 2010
LPS-stimulated human bone marrow stroma cells support myeloid cell development and progenitor cell maintenance Ziegler, Patrick; Boettcher, Steffen; Takizawa, Hitoshi Annals of Hematology, Vol. 95, Issue 2 https://doi.org/10.1007/s00277-015-2550-5	journal	November 2015
Enhancing the Therapeutic Potential of Mesenchymal Stem Cells with the CRISPR-Cas System Filho, Daniel Mendes; de Carvalho Ribeiro, Patrícia; Oliveira, Lucas Felipe Stem Cell Reviews and Reports, Vol. 15, Issue 4 https://doi.org/10.1007/s12015-019-09897-0	journal	May 2019
CD14 Is a pattern recognition receptor Pugin, Jérôme; Heumann, Didier; Tomasz, Alexander Immunity, Vol. 1, Issue 6 https://doi.org/10.1016/1074-7613(94)90093-0	journal	September 1994
Resistance to Endotoxin Shock and Reduced Dissemination of Gram-Negative Bacteria in CD14-Deficient Mice Haziot, Alain; Ferrero, Enza; Köntgen, Frank Immunity, Vol. 4, Issue 4 https://doi.org/10.1016/S1074-7613(00)80254-X	journal	April 1996
CD14, new aspects of ligand and signal diversity Landmann, Regine; Müller, Beat; Zimmerli, Werner Microbes and Infection, Vol. 2, Issue 3 https://doi.org/10.1016/S1286-4579(00)00298-7	journal	March 2000
Priming of Toll-like receptor 4 pathway in mesenchymal stem cells increases expression of B cell activating factor Yan, Hao; Wu, Mengyao; Yuan, Yan Biochemical and Biophysical Research Communications, Vol. 448, Issue 2 https://doi.org/10.1016/j.bbrc.2014.04.097	journal	May 2014
Primed mesenchymal stem cells package exosomes with metabolites associated with immunomodulation Showalter, Megan R.; Wancewicz, Benjamin; Fiehn, Oliver Biochemical and Biophysical Research Communications, Vol. 512, Issue 4 https://doi.org/10.1016/j.bbrc.2019.03.119	journal	May 2019
A Unifying Model for the Selective Regulation of Inducible Transcription by CpG Islands and Nucleosome Remodeling Ramirez-Carrozzi, Vladimir R.; Braas, Daniel; Bhatt, Dev M. Cell, Vol. 138, Issue 1 https://doi.org/10.1016/j.cell.2009.04.020	journal	July 2009
Comprehensive Integration of Single-Cell Data Stuart, Tim; Butler, Andrew; Hoffman, Paul Cell, Vol. 177, Issue 7 https://doi.org/10.1016/j.cell.2019.05.031	journal	June 2019
Cellular Decision Making by Non-Integrative Processing of TLR Inputs Kellogg, Ryan A.; Tian, Chengzhe; Etzrodt, Martin Cell Reports, Vol. 19, Issue 1 https://doi.org/10.1016/j.celrep.2017.03.027	journal	April 2017
Mapping Distinct Bone Marrow Niche Populations and Their Differentiation Paths Wolock, Samuel L.; Krishnan, Indira; Tenen, Danielle E. Cell Reports, Vol. 28, Issue 2 https://doi.org/10.1016/j.celrep.2019.06.031	journal	July 2019
LPS/TLR4 signal transduction pathway Lu, Yong-Chen; Yeh, Wen-Chen; Ohashi, Pamela S. Cytokine, Vol. 42, Issue 2 https://doi.org/10.1016/j.cyto.2008.01.006	journal	May 2008
Mesenchymal stromal cells and the innate immune response Le Blanc, Katarina; Davies, Lindsay C. Immunology Letters, Vol. 168, Issue 2 https://doi.org/10.1016/j.imlet.2015.05.004	journal	December 2015
Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-like Receptor Ligands Shi, Chao; Jia, Ting; Mendez-Ferrer, Simon Immunity, Vol. 34, Issue 4 https://doi.org/10.1016/j.immuni.2011.02.016	journal	April 2011
Gene regulation in the immediate-early response process Bahrami, Shahram; Drabløs, Finn Advances in Biological Regulation, Vol. 62 https://doi.org/10.1016/j.jbior.2016.05.001	journal	September 2016
Regulation of Primary Response Genes Fowler, Trent; Sen, Ranjan; Roy, Ananda L. Molecular Cell, Vol. 44, Issue 3 https://doi.org/10.1016/j.molcel.2011.09.014	journal	November 2011
The contributing role of CD14 in toll-like receptor 4 dependent neuropathic pain Cao, L.; Tanga, F. Y.; DeLeo, J. A. Neuroscience, Vol. 158, Issue 2 https://doi.org/10.1016/j.neuroscience.2008.10.004	journal	January 2009
Mesenchymal Stromal Cells: Clinical Challenges and Therapeutic Opportunities Galipeau, Jacques; Sensébé, Luc Cell Stem Cell, Vol. 22, Issue 6 https://doi.org/10.1016/j.stem.2018.05.004	journal	June 2018
Toll-like receptors 2 and 4 mediate the capacity of mesenchymal stromal cells to support the proliferation and differentiation of CD34+ cells Wang, Xingbing; Cheng, Qiansong; Li, Lailing Experimental Cell Research, Vol. 318, Issue 3 https://doi.org/10.1016/j.yexcr.2011.11.001	journal	February 2012
The role of nitric oxide in innate immunity: NO in innate immunity Bogdan, Christian; Röllinghoff, Martin; Diefenbach, Andreas Immunological Reviews, Vol. 173, Issue 1 https://doi.org/10.1034/j.1600-065X.2000.917307.x	journal	February 2000
Gene Ontology: tool for the unification of biology Ashburner, Michael; Ball, Catherine A.; Blake, Judith A. Nature Genetics, Vol. 25, Issue 1 https://doi.org/10.1038/75556	journal	May 2000
A subset of IL-17+ mesenchymal stem cells possesses anti-Candida albicans effect Yang, Ruili; Liu, Yi; Kelk, Peyman Cell Research, Vol. 23, Issue 1 https://doi.org/10.1038/cr.2012.179	journal	December 2012
Recognition of lipopolysaccharide pattern by TLR4 complexes Park, Beom Seok; Lee, Jie-Oh Experimental & Molecular Medicine, Vol. 45, Issue 12 https://doi.org/10.1038/emm.2013.97	journal	December 2013
TLR3 preconditioning enhances the therapeutic efficacy of umbilical cord mesenchymal stem cells in TNBS-induced colitis via the TLR3-Jagged-1-Notch-1 pathway Qiu, Y.; Guo, J.; Mao, R. Mucosal Immunology, Vol. 10, Issue 3 https://doi.org/10.1038/mi.2016.78	journal	September 2016
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex Konermann, Silvana; Brigham, Mark D.; Trevino, Alexandro E. Nature, Vol. 517, Issue 7536 https://doi.org/10.1038/nature14136	journal	December 2014
The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells Trapnell, Cole; Cacchiarelli, Davide; Grimsby, Jonna Nature Biotechnology, Vol. 32, Issue 4 https://doi.org/10.1038/nbt.2859	journal	March 2014
Near-optimal probabilistic RNA-seq quantification Bray, Nicolas L.; Pimentel, Harold; Melsted, Páll Nature Biotechnology, Vol. 34, Issue 5 https://doi.org/10.1038/nbt.3519	journal	April 2016
Integrating single-cell transcriptomic data across different conditions, technologies, and species Butler, Andrew; Hoffman, Paul; Smibert, Peter Nature Biotechnology, Vol. 36, Issue 5 https://doi.org/10.1038/nbt.4096	journal	April 2018
Dimensionality reduction for visualizing single-cell data using UMAP Becht, Etienne; McInnes, Leland; Healy, John Nature Biotechnology, Vol. 37, Issue 1 https://doi.org/10.1038/nbt.4314	journal	December 2018
Plasticity of mesenchymal stem cells in immunomodulation: pathological and therapeutic implications Wang, Ying; Chen, Xiaodong; Cao, Wei Nature Immunology, Vol. 15, Issue 11 https://doi.org/10.1038/ni.3002	journal	October 2014
Reversed graph embedding resolves complex single-cell trajectories Qiu, Xiaojie; Mao, Qi; Tang, Ying Nature Methods, Vol. 14, Issue 10 https://doi.org/10.1038/nmeth.4402	journal	August 2017
The MaxQuant computational platform for mass spectrometry-based shotgun proteomics Tyanova, Stefka; Temu, Tikira; Cox, Juergen Nature Protocols, Vol. 11, Issue 12 https://doi.org/10.1038/nprot.2016.136	journal	October 2016
Proteome-wide identification of ubiquitin interactions using UbIA-MS Zhang, Xiaofei; Smits, Arne H.; van Tilburg, Gabrielle BA Nature Protocols, Vol. 13, Issue 3 https://doi.org/10.1038/nprot.2017.147	journal	February 2018
Hypoxia preconditioning promotes bone marrow mesenchymal stem cells survival by inducing HIF-1α in injured neuronal cells derived exosomes culture system Luo, Zucheng; Wu, Fangfang; Xue, Enxing Cell Death & Disease, Vol. 10, Issue 2 https://doi.org/10.1038/s41419-019-1410-y	journal	February 2019
Single cell transcriptomic analysis of human mesenchymal stem cells reveals limited heterogeneity Huang, Yin; Li, Qing; Zhang, Kunshan Cell Death & Disease, Vol. 10, Issue 5 https://doi.org/10.1038/s41419-019-1583-4	journal	May 2019
Mesenchymal stem cell perspective: cell biology to clinical progress Pittenger, Mark F.; Discher, Dennis E.; Péault, Bruno M. npj Regenerative Medicine, Vol. 4, Issue 1 https://doi.org/10.1038/s41536-019-0083-6	journal	December 2019
Manufacturing of primed mesenchymal stromal cells for therapy Yin, James Q.; Zhu, Jun; Ankrum, James A. Nature Biomedical Engineering, Vol. 3, Issue 2 https://doi.org/10.1038/s41551-018-0325-8	journal	January 2019
The single-cell transcriptional landscape of mammalian organogenesis Cao, Junyue; Spielmann, Malte; Qiu, Xiaojie Nature, Vol. 566, Issue 7745 https://doi.org/10.1038/s41586-019-0969-x	journal	February 2019
Activated Mesenchymal Stem Cells Interact with Antibiotics and Host Innate Immune Responses to Control Chronic Bacterial Infections Johnson, Valerie; Webb, Tracy; Norman, Annalis Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-08311-4	journal	August 2017
NF-κB signaling in inflammation Liu, Ting; Zhang, Lingyun; Joo, Donghyun Signal Transduction and Targeted Therapy, Vol. 2, Issue 1 https://doi.org/10.1038/sigtrans.2017.23	journal	July 2017
TLR3-/4-Priming Differentially Promotes Ca2+ Signaling and Cytokine Expression and Ca2+-Dependently Augments Cytokine Release in hMSCs Park, Kyoung Sun; Kim, Sun Hwa; Das, Amitabh Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep23103	journal	March 2016
Interferon-γ and Tumor Necrosis Factor-α Polarize Bone Marrow Stromal Cells Uniformly to a Th1 Phenotype Jin, Ping; Zhao, Yuanlong; Liu, Hui Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep26345	journal	May 2016
Transcriptome sequencing wide functional analysis of human mesenchymal stem cells in response to TLR4 ligand Kim, Sun Hwa; Das, Amitabh; Chai, Jin Choul Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep30311	journal	July 2016
CD14 dictates differential activation of mesenchymal stromal cells through AKT, NF-κB and P38 signals Jiang, Menghui; Gao, Tianlin; Liu, Yuansheng Bioscience Reports, Vol. 39, Issue 7 https://doi.org/10.1042/BSR20190807	journal	July 2019
Isolation of an endotoxin-MD-2 complex that produces Toll-like receptor 4-dependent cell activation at picomolar concentrations Gioannini, T. L.; Teghanemt, A.; Zhang, D. Proceedings of the National Academy of Sciences, Vol. 101, Issue 12 https://doi.org/10.1073/pnas.0306906101	journal	March 2004
Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties Bartosh, T. J.; Ylostalo, J. H.; Mohammadipoor, A. Proceedings of the National Academy of Sciences, Vol. 107, Issue 31 https://doi.org/10.1073/pnas.1008117107	journal	July 2010
Transcriptional Roles of Nuclear Factor κB and Nuclear Factor-Interleukin-6 in the Tumor Necrosis Factor α-Dependent Induction of Cyclooxygenase-2 in MC3T3-E1 Cells Yamamoto, Kei; Arakawa, Toshiya; Ueda, Natsuo Journal of Biological Chemistry, Vol. 270, Issue 52 https://doi.org/10.1074/jbc.270.52.31315	journal	December 1995
Phosphatidylinositides Bind to Plasma Membrane CD14 and Can Prevent Monocyte Activation by Bacterial Lipopolysaccharide Wang, Ping-yuan; Kitchens, Richard L.; Munford, Robert S. Journal of Biological Chemistry, Vol. 273, Issue 38 https://doi.org/10.1074/jbc.273.38.24309	journal	September 1998
Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement Dominici, M.; Le Blanc, K.; Mueller, I. Cytotherapy, Vol. 8, Issue 4 https://doi.org/10.1080/14653240600855905	journal	January 2006
Hypoxia/Reoxygenation Differentially Modulates NF-κB Activation and iNOS Expression in Astrocytes and Microglia Guo, Guiwen; Bhat, Narayan R. Antioxidants & Redox Signaling, Vol. 8, Issue 5-6 https://doi.org/10.1089/ars.2006.8.911	journal	May 2006
edgeR: a Bioconductor package for differential expression analysis of digital gene expression data Robinson, M. D.; McCarthy, D. J.; Smyth, G. K. Bioinformatics, Vol. 26, Issue 1 https://doi.org/10.1093/bioinformatics/btp616	journal	November 2009
Pathview: an R/Bioconductor package for pathway-based data integration and visualization Luo, Weijun; Brouwer, Cory Bioinformatics, Vol. 29, Issue 14 https://doi.org/10.1093/bioinformatics/btt285	journal	June 2013
fastp: an ultra-fast all-in-one FASTQ preprocessor Chen, Shifu; Zhou, Yanqing; Chen, Yaru Bioinformatics, Vol. 34, Issue 17 https://doi.org/10.1093/bioinformatics/bty560	journal	September 2018
Adjusting batch effects in microarray expression data using empirical Bayes methods Johnson, W. Evan; Li, Cheng; Rabinovic, Ariel Biostatistics, Vol. 8, Issue 1 https://doi.org/10.1093/biostatistics/kxj037	journal	April 2006
PANTHER version 14: more genomes, a new PANTHER GO-slim and improvements in enrichment analysis tools Mi, Huaiyu; Muruganujan, Anushya; Ebert, Dustin Nucleic Acids Research, Vol. 47, Issue D1 https://doi.org/10.1093/nar/gky1038	journal	November 2018
Nuclear Factor-Kappa B Regulates Inducible Prostaglandin E Synthase Expression in Human Amnion Mesenchymal Cells1 Ackerman, William E.; Summerfield, Taryn L. S.; Vandre, Dale D. Biology of Reproduction, Vol. 78, Issue 1 https://doi.org/10.1095/biolreprod.107.061663	journal	January 2008
Innate Immune Response in th1- and Th2-Dominant Mouse Strains Watanabe, Hiroyuki; Numata, Kousuke; Ito, Takaaki Shock, Vol. 22, Issue 5 https://doi.org/10.1097/01.shk.0000142249.08135.e9	journal	January 2004
Antibacterial effect of mesenchymal stem cells against Escherichia coli is mediated by secretion of beta‐ defensin‐ 2 via toll‐ like receptor 4 signalling Sung, Dong Kyung; Chang, Yun Sil; Sung, Se In Cellular Microbiology, Vol. 18, Issue 3 https://doi.org/10.1111/cmi.12522	journal	October 2015
Signal transduction by the lipopolysaccharide receptor, Toll-like receptor-4 Palsson-McDermott, Eva M.; O'Neill, Luke A. J. Immunology, Vol. 113, Issue 2 https://doi.org/10.1111/j.1365-2567.2004.01976.x	journal	October 2004
In vivo priming of human mesenchymal stem cells with hepatocyte growth factor–engineered mesenchymal stem cells promotes therapeutic potential for cardiac repair Park, Bong-Woo; Jung, Soo-Hyun; Das, Sanskrita Science Advances, Vol. 6, Issue 13 https://doi.org/10.1126/sciadv.aay6994	journal	March 2020
Multilineage Potential of Adult Human Mesenchymal Stem Cells Pittenger, M. F. Science, Vol. 284, Issue 5411 https://doi.org/10.1126/science.284.5411.143	journal	April 1999
Role of the NF-κB Signaling Pathway and κB cis -Regulatory Elements on the IRF-1 and iNOS Promoter Regions in Mycobacterial Lipoarabinomannan Induction of Nitric Oxide Morris, Kristin R.; Lutz, Ryan D.; Choi, Hyung-Seok Infection and Immunity, Vol. 71, Issue 3 https://doi.org/10.1128/IAI.71.3.1442-1452.2003	journal	March 2003
Mesenchymal stem cells enhance survival and bacterial clearance in murine Escherichia coli pneumonia Gupta, Naveen; Krasnodembskaya, Anna; Kapetanaki, Maria Thorax, Vol. 67, Issue 6 https://doi.org/10.1136/thoraxjnl-2011-201176	journal	January 2012
Molecular Basis of NF-κB Signaling Napetschnig, Johanna; Wu, Hao Annual Review of Biophysics, Vol. 42, Issue 1 https://doi.org/10.1146/annurev-biophys-083012-130338	journal	May 2013
Antimicrobial Properties of Mesenchymal Stem Cells: Therapeutic Potential for Cystic Fibrosis Infection, and Treatment Sutton, Morgan T.; Fletcher, David; Ghosh, Santosh K. Stem Cells International, Vol. 2016 https://doi.org/10.1155/2016/5303048	journal	January 2016
Comparative Study on In Vitro Culture of Mouse Bone Marrow Mesenchymal Stem Cells Hu, Yuxin; Lou, Bin; Wu, Xiafang Stem Cells International, Vol. 2018 https://doi.org/10.1155/2018/6704583	journal	January 2018
Single-Cell RNA-Sequencing and Metabolomics Analyses Reveal the Contribution of Perivascular Adipose Tissue Stem Cells to Vascular Remodeling Gu, Wenduo; Nowak, Witold N.; Xie, Yao Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 39, Issue 10 https://doi.org/10.1161/ATVBAHA.119.312732	journal	October 2019
Lipopolysaccharide binding protein and soluble CD14 catalyze exchange of phospholipids. Yu, B.; Hailman, E.; Wright, S. D. Journal of Clinical Investigation, Vol. 99, Issue 2 https://doi.org/10.1172/JCI119160	journal	January 1997
Immunological Variation Between Inbred Laboratory Mouse Strains: Points to Consider in Phenotyping Genetically Immunomodified Mice Sellers, R. S.; Clifford, C. B.; Treuting, P. M. Veterinary Pathology, Vol. 49, Issue 1 https://doi.org/10.1177/0300985811429314	journal	November 2011
Toll-like receptors and their ligands control mesenchymal stem cell functions Pevsner-Fischer, Meirav; Morad, Vered; Cohen-Sfady, Michal Blood, Vol. 109, Issue 4 https://doi.org/10.1182/blood-2006-06-028704	journal	October 2006
Lipocalin 2 is protective against E. coli pneumonia Wu, Hong; Santoni-Rugiu, Eric; Ralfkiaer, Elisabeth Respiratory Research, Vol. 11, Issue 1 https://doi.org/10.1186/1465-9921-11-96	journal	July 2010
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 Love, Michael I.; Huber, Wolfgang; Anders, Simon Genome Biology, Vol. 15, Issue 12 https://doi.org/10.1186/s13059-014-0550-8	journal	December 2014
A benchmark of batch-effect correction methods for single-cell RNA sequencing data Tran, Hoa Thi Nhu; Ang, Kok Siong; Chevrier, Marion Genome Biology, Vol. 21, Issue 1 https://doi.org/10.1186/s13059-019-1850-9	journal	January 2020
Combination therapy of menstrual derived mesenchymal stem cells and antibiotics ameliorates survival in sepsis Alcayaga-Miranda, Francisca; Cuenca, Jimena; Martin, Aldo Stem Cell Research & Therapy, Vol. 6, Issue 1 https://doi.org/10.1186/s13287-015-0192-0	journal	October 2015
Changes in the secretome of tri-dimensional spheroid-cultured human mesenchymal stem cells in vitro by interleukin-1 priming Redondo-Castro, Elena; Cunningham, Catriona J.; Miller, Jonjo Stem Cell Research & Therapy, Vol. 9, Issue 1 https://doi.org/10.1186/s13287-017-0753-5	journal	January 2018
Priming approaches to improve the efficacy of mesenchymal stromal cell-based therapies Noronha, Nádia de Cássia; Mizukami, Amanda; Caliári-Oliveira, Carolina Stem Cell Research & Therapy, Vol. 10, Issue 1 https://doi.org/10.1186/s13287-019-1224-y	journal	May 2019
Single-cell RNA-seq highlights heterogeneity in human primary Wharton’s jelly mesenchymal stem/stromal cells cultured in vitro Sun, Changbin; Wang, Lei; Wang, Hailun Stem Cell Research & Therapy, Vol. 11, Issue 1 https://doi.org/10.1186/s13287-020-01660-4	journal	April 2020
Spermatogonial Cell-Mediated Activation of an IκBζ-Independent Nuclear Factor-κB Pathway in Sertoli Cells Induces Transcription of the Lipocalin-2 Gene Fujino, Ryu-Suke; Tanaka, Kiyoko; Morimatsu, Masami Molecular Endocrinology, Vol. 20, Issue 4 https://doi.org/10.1210/me.2005-0423	journal	April 2006
A New Mesenchymal Stem Cell (MSC) Paradigm: Polarization into a Pro-Inflammatory MSC1 or an Immunosuppressive MSC2 Phenotype Waterman, Ruth S.; Tomchuck, Suzanne L.; Henkle, Sarah L. PLoS ONE, Vol. 5, Issue 4 https://doi.org/10.1371/journal.pone.0010088	journal	April 2010
Single-Cell RNA-Seq of Bone Marrow-Derived Mesenchymal Stem Cells Reveals Unique Profiles of Lineage Priming Freeman, Brian T.; Jung, Jangwook P.; Ogle, Brenda M. PLOS ONE, Vol. 10, Issue 9 https://doi.org/10.1371/journal.pone.0136199	journal	September 2015
Hypoxia-preconditioned mesenchymal stem cells ameliorate ischemia/reperfusion-induced lung injury Liu, Yung-Yang; Chiang, Chi-Huei; Hung, Shih-Chieh PLOS ONE, Vol. 12, Issue 11 https://doi.org/10.1371/journal.pone.0187637	journal	November 2017
TLR4‐dependent shaping of the wound site by MSCs accelerates wound healing Munir, Saira; Basu, Abhijit; Maity, Pallab EMBO reports, Vol. 21, Issue 5 https://doi.org/10.15252/embr.201948777	journal	March 2020
Toll-Like Receptors 3 and 4 Are Expressed by Human Bone Marrow-Derived Mesenchymal Stem Cells and Can Inhibit Their T-Cell Modulatory Activity by Impairing Notch Signaling Liotta, Francesco; Angeli, Roberta; Cosmi, Lorenzo Stem Cells, Vol. 26, Issue 1 https://doi.org/10.1634/stemcells.2007-0454	journal	October 2007
Toll-Like Receptors on Human Mesenchymal Stem Cells Drive Their Migration and Immunomodulating Responses Tomchuck, Suzanne L.; Zwezdaryk, Kevin J.; Coffelt, Seth B. Stem Cells, Vol. 26, Issue 1 https://doi.org/10.1634/stemcells.2007-0563	journal	October 2007
Genetic Engineering as a Strategy to Improve the Therapeutic Efficacy of Mesenchymal Stem/Stromal Cells in Regenerative Medicine Damasceno, Patricia Kauanna Fonseca; de Santana, Thaís Alves; Santos, Girlaine Café Frontiers in Cell and Developmental Biology, Vol. 8 https://doi.org/10.3389/fcell.2020.00737	journal	August 2020
Role of CD14 in host protection against infections and in metabolism regulation Zanoni, Ivan; Granucci, Francesca Frontiers in Cellular and Infection Microbiology, Vol. 3 https://doi.org/10.3389/fcimb.2013.00032	journal	January 2013
Toll-Like Receptor Signaling Pathways Kawasaki, Takumi; Kawai, Taro Frontiers in Immunology, Vol. 5 https://doi.org/10.3389/fimmu.2014.00461	journal	September 2014
Antimicrobial Activity of Mesenchymal Stem Cells: Current Status and New Perspectives of Antimicrobial Peptide-Based Therapies Alcayaga-Miranda, Francisca; Cuenca, Jimena; Khoury, Maroun Frontiers in Immunology, Vol. 8 https://doi.org/10.3389/fimmu.2017.00339	journal	March 2017
Multiplicity of Mesenchymal Stromal Cells: Finding the Right Route to Therapy Wilson, Alison; Hodgson-Garms, Margeaux; Frith, Jessica E. Frontiers in Immunology, Vol. 10 https://doi.org/10.3389/fimmu.2019.01112	journal	May 2019
Enhancing the Therapeutic Potential of CCL2-Overexpressing Mesenchymal Stem Cells in Acute Stroke Lee, Sanghun; Kim, Ok Joon; Lee, Kee Ook International Journal of Molecular Sciences, Vol. 21, Issue 20 https://doi.org/10.3390/ijms21207795	journal	October 2020
Cutting Edge: Heat Shock Protein (HSP) 60 Activates the Innate Immune Response: CD14 Is an Essential Receptor for HSP60 Activation of Mononuclear Cells Kol, Amir; Lichtman, Andrew H.; Finberg, Robert W. The Journal of Immunology, Vol. 164, Issue 1 https://doi.org/10.4049/jimmunol.164.1.13	journal	January 2000
Mesenchymal Stromal Cells and Toll-Like Receptor Priming: A Critical Review Najar, Mehdi; Krayem, Mohammad; Meuleman, Nathalie Immune Network, Vol. 17, Issue 2 https://doi.org/10.4110/in.2017.17.2.89	journal	January 2017
Increased Survival and Function of Mesenchymal Stem Cell Spheroids Entrapped in Instructive Alginate Hydrogels Ho, Steve S.; Murphy, Kaitlin C.; Binder, Bernard Y. K. Stem Cells Translational Medicine, Vol. 5, Issue 6 https://doi.org/10.5966/sctm.2015-0211	journal	April 2016

Similar Records

Augmentation of Antibacterial Activity in Mesenchymal Stromal Cells Through Systems-Level Analysis and CRISPR-mediated Activation of CD14.

Conference · 2021 · OSTI ID:1868446

Hirakawa, Matthew; Tjahjono, Nikki; Light, Yooli Kim; +4 more

Lipopolysaccharide induces proliferation and osteogenic differentiation of adipose-derived mesenchymal stromal cells in vitro via TLR4 activation

Journal Article · 2016 · Experimental Cell Research · OSTI ID:22649803

Herzmann, Nicole; Salamon, Achim; Fiedler, Tomas; +1 more

Chromosomal stability of mesenchymal stromal cells during in vitro culture

Journal Article · 2016 · Cytotherapy · OSTI ID:1357294

Stultz, Brian G.; McGinnis, Kathleen; Thompson, Elaine E.; +3 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Cell biology
Molecular biology
Omics
Science & Technology

Title: Upregulation of CD14 in mesenchymal stromal cells accelerates lipopolysaccharide-induced response and enhances antibacterial properties

Citation Formats

References (98)

Similar Records

Related Subjects