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Title: Scanning structural mapping at the Life Science X-ray Scattering Beamline

Abstract

This work describes the instrumentation and software for microbeam scattering and structural mapping at the Life Science X-ray Scattering (LiX) beamline at NSLS-II. Using a two-stage focusing scheme, an adjustable beam size between a few micrometres and a fraction of a millimetre is produced at the sample position. Scattering data at small and wide angles are collected simultaneously on multiple Pilatus detectors. A recent addition of an in-vacuum Pilatus 900k detector, with the detector modules arranged in a C-shaped configuration, has improved the azimuthal angle coverage in the wide-angle data. As an option, fluorescence data can be collected simultaneously. Fly scans have been implemented to minimize the time interval between scattering patterns and to avoid unnecessary radiation damage to the sample. For weakly scattering samples, an in-vacuum sample environment has been developed here to minimize background scattering. Data processing for these measurements is highly sample-specific. To establish a generalized data process workflow, first the data are reduced to reciprocal coordinates at the time of data collection. The users can then quantify features of their choosing from these intermediate data and construct structural maps. As examples, results from in-vacuum mapping of onion epidermal cell walls and 2D tomographic sectioning of anmore » intact poplar stem are presented.« less

Authors:
ORCiD logo; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH); National Institute of General Medical Sciences (NIGMS); USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1840613
Alternate Identifier(s):
OSTI ID: 1844570
Report Number(s):
BNL-222733-2022-JAAM
Journal ID: ISSN 1600-5775; JSYRES; PII: S1600577521013266
Grant/Contract Number:  
KP1605010; SC0012704; P30GM133893; S10 OD012331
Resource Type:
Published Article
Journal Name:
Journal of Synchrotron Radiation (Online)
Additional Journal Information:
Journal Name: Journal of Synchrotron Radiation (Online) Journal Volume: 29 Journal Issue: 2; Journal ID: ISSN 1600-5775
Publisher:
International Union of Crystallography (IUCr)
Country of Publication:
Denmark
Language:
English
Subject:
36 MATERIALS SCIENCE; structural mapping; imaging; SAXS/WAXS

Citation Formats

Yang, Lin, Liu, Jiliang, Chodankar, Shirish, Antonelli, Stephen, and DiFabio, Jonathan. Scanning structural mapping at the Life Science X-ray Scattering Beamline. Denmark: N. p., 2022. Web. doi:10.1107/S1600577521013266.
Yang, Lin, Liu, Jiliang, Chodankar, Shirish, Antonelli, Stephen, & DiFabio, Jonathan. Scanning structural mapping at the Life Science X-ray Scattering Beamline. Denmark. https://doi.org/10.1107/S1600577521013266
Yang, Lin, Liu, Jiliang, Chodankar, Shirish, Antonelli, Stephen, and DiFabio, Jonathan. Mon . "Scanning structural mapping at the Life Science X-ray Scattering Beamline". Denmark. https://doi.org/10.1107/S1600577521013266.
@article{osti_1840613,
title = {Scanning structural mapping at the Life Science X-ray Scattering Beamline},
author = {Yang, Lin and Liu, Jiliang and Chodankar, Shirish and Antonelli, Stephen and DiFabio, Jonathan},
abstractNote = {This work describes the instrumentation and software for microbeam scattering and structural mapping at the Life Science X-ray Scattering (LiX) beamline at NSLS-II. Using a two-stage focusing scheme, an adjustable beam size between a few micrometres and a fraction of a millimetre is produced at the sample position. Scattering data at small and wide angles are collected simultaneously on multiple Pilatus detectors. A recent addition of an in-vacuum Pilatus 900k detector, with the detector modules arranged in a C-shaped configuration, has improved the azimuthal angle coverage in the wide-angle data. As an option, fluorescence data can be collected simultaneously. Fly scans have been implemented to minimize the time interval between scattering patterns and to avoid unnecessary radiation damage to the sample. For weakly scattering samples, an in-vacuum sample environment has been developed here to minimize background scattering. Data processing for these measurements is highly sample-specific. To establish a generalized data process workflow, first the data are reduced to reciprocal coordinates at the time of data collection. The users can then quantify features of their choosing from these intermediate data and construct structural maps. As examples, results from in-vacuum mapping of onion epidermal cell walls and 2D tomographic sectioning of an intact poplar stem are presented.},
doi = {10.1107/S1600577521013266},
journal = {Journal of Synchrotron Radiation (Online)},
number = 2,
volume = 29,
place = {Denmark},
year = {Mon Jan 17 00:00:00 EST 2022},
month = {Mon Jan 17 00:00:00 EST 2022}
}

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