A Structural Perspective on the RNA Editing of Plant Respiratory Complexes

Maldonado, Maria; Abe, Kaitlyn Madison; Letts, James Anthony

doi:10.3390/ijms23020684

Title: A Structural Perspective on the RNA Editing of Plant Respiratory Complexes

Journal Article · 08 January 2022 · International Journal of Molecular Sciences (Online)

DOI: https://doi.org/10.3390/ijms23020684 · OSTI ID:1839126

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The last steps of respiration, a core energy-harvesting process, are carried out by a chain of multi-subunit complexes in the inner mitochondrial membrane. Several essential subunits of the respiratory complexes are RNA-edited in plants, frequently leading to changes in the encoded amino acids. While the impact of RNA editing is clear at the sequence and phenotypic levels, the underlying biochemical explanations for these effects have remained obscure. Here, we used the structures of plant respiratory complex I, complex III2 and complex IV to analyze the impact of the amino acid changes of RNA editing in terms of their location and biochemical features. Through specific examples, we demonstrate how the structural information can explain the phenotypes of RNA-editing mutants. This work shows how the structural perspective can bridge the gap between sequence and phenotype and provides a framework for the continued analysis of RNA-editing mutants in plant mitochondria and, by extension, in chloroplasts.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0022293

OSTI ID:: 1839126

Journal Information:: International Journal of Molecular Sciences (Online), Journal Name: International Journal of Molecular Sciences (Online) Journal Issue: 2 Vol. 23; ISSN 1422-0067; ISSN IJMCFK

Publisher:: MDPI AGCopyright Statement

Country of Publication:: Switzerland

Language:: English

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