Layer‐by‐Layer Engineered All‐Liquid Microfluidic Chips for Enzyme Immobilization

Liu, Tan; Yin, Yixuan; Yang, Yang; Russell, Thomas P.; Shi, Shaowei

doi:10.1002/adma.202105386

Title: Layer‐by‐Layer Engineered All‐Liquid Microfluidic Chips for Enzyme Immobilization

Journal Article · 22 December 2021 · Advanced Materials

DOI: https://doi.org/10.1002/adma.202105386 · OSTI ID:1837313

Liu, Tan ^[1]; Yin, Yixuan ^[1]; Yang, Yang ^[1];

^[2]; Shi, Shaowei ^[1]

Beijing Advanced Innovation Center for Soft Matter Science and Engineering College of Materials Science and Engineering Beijing University of Chemical Technology Beijing 100029 China
Department of Polymer Science and Engineering University of Massachusetts Amherst MA 01003 USA, Materials Sciences Division Lawrence Berkeley National Laboratory 1 Cyclotron Road Berkeley CA 94720 USA

Abstract Enzyme immobilization in the confines of microfluidic chips, that promote enzyme activity and stability, has become a powerful strategy to enhance biocatalysis and biomass conversion. Here, based on a newly developed all‐liquid microfluidic chip, fabricated by the interfacial assembly of nanoparticle surfactants (NPSs) in a biphasic system, a layer‐by‐layer assembly strategy to generate polysaccharide multilayers on the surface of a microchannel, greatly enhancing the mechanical properties of the microchannel and offering a biocompatible microenvironment for enzyme immobilization, is presented. Using horseradish peroxidase and glucose oxidase as model enzymes, all‐liquid microfluidic enzymatic and cascade reactors have been constructed and the crucial role of polysaccharide multilayers on enhancing the enzyme loading and catalytic efficiency is demonstrated.

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Sponsoring Organization:: USDOE

OSTI ID:: 1837313

Journal Information:: Advanced Materials, Journal Name: Advanced Materials Journal Issue: 5 Vol. 34; ISSN 0935-9648

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Germany

Language:: English

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