Controlled Organization of Inorganic Materials Using Biological Molecules for Activating Therapeutic Functionalities

Chandler, Morgan; Minevich, Brian; Roark, Brandon; Viard, Mathias; Johnson, M. Brittany; Rizvi, Mehedi H.; Deaton, Thomas A.; Kozlov, Seraphim; Panigaj, Martin; Tracy, Joseph B.; Yingling, Yaroslava G.; Gang, Oleg; Afonin, Kirill A.

doi:10.1021/acsami.1c09230

Title: Controlled Organization of Inorganic Materials Using Biological Molecules for Activating Therapeutic Functionalities

Journal Article · 16 August 2021 · ACS Applied Materials and Interfaces

DOI: https://doi.org/10.1021/acsami.1c09230 · OSTI ID:1837208

^[1]; Minevich, Brian ^[2]; Roark, Brandon ^[1]; Viard, Mathias ^[3]; Johnson, M. Brittany ^[1]; Rizvi, Mehedi H. ^[4]; Deaton, Thomas A. ^[4]; Kozlov, Seraphim ^[1]; Panigaj, Martin ^[5];

^[4];

^[6];

^[1]

Univ. of North Carolina, Charlotte, NC (United States)
Columbia Univ., New York, NY (United States)
Frederick National Lab. for Cancer Research, Frederick, MD (United States)
North Carolina State Univ., Raleigh, NC (United States)
Pavol Jozef Safarik University, Kosice (Slovak Republic)
Columbia Univ., New York, NY (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)

Precise control over the assembly of biocompatible three-dimensional (3D) nanostructures would allow for programmed interactions within the cellular environment. Nucleic acids can be used as programmable crosslinkers to direct the assembly of quantum dots (QDs) and tuned to demonstrate different interparticle binding strategies. In this work, morphologies of self-assembled QDs are evaluated via gel electrophoresis, transmission electron microscopy, small-angle X-ray scattering, and dissipative particle dynamics simulations, with all results being in good agreement. The controlled assembly of 3D QD organizations is demonstrated in cells via the colocalized emission of multiple assembled QDs, and their immunorecognition is assessed via enzyme-linked immunosorbent assays. RNA interference inducers are also embedded into the interparticle binding strategy to be released in human cells only upon QD assembly, which is demonstrated by specific gene silencing. The programmability and intracellular activity of QD assemblies offer a strategy for nucleic acids to imbue the structure and therapeutic function into the formation of complex networks of nanostructures, while the photoluminescent properties of the material allow for optical tracking in cells in vitro.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: SC0012704

OSTI ID:: 1837208

Report Number(s):: BNL-222519-2021-JAAM

Journal Information:: ACS Applied Materials and Interfaces, Journal Name: ACS Applied Materials and Interfaces Journal Issue: 33 Vol. 13; ISSN 1944-8244

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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36 MATERIALS SCIENCE
3D assemblies
bioimaging
materials organization
programmable assembly
quantum dots
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therapeutic nucleic acids

Title: Controlled Organization of Inorganic Materials Using Biological Molecules for Activating Therapeutic Functionalities

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