High-resolution cryo-electron microscopy structure of photosystem II from the mesophilic cyanobacterium, Synechocystis sp. PCC 6803

Gisriel, Christopher J.; Wang, Jimin; Liu, Jinchan; Flesher, David A.; Reiss, Krystle M.; Huang, Hao-Li; Yang, Ke R.; Armstrong, William H.; Gunner, M. R.; Batista, Victor S.; Debus, Richard J.; Brudvig, Gary W.

doi:10.1073/pnas.2116765118

Title: High-resolution cryo-electron microscopy structure of photosystem II from the mesophilic cyanobacterium, Synechocystis sp. PCC 6803

Journal Article · 21 December 2021 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.2116765118 · OSTI ID:1837119

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^[2];

^[2]; Reiss, Krystle M. ^[1]; Huang, Hao-Li ^[1]; Yang, Ke R. ^[1];

^[3]; Gunner, M. R. ^[4];

^[1];

^[5];

^[6]

Department of Chemistry, Yale University, New Haven, CT 06520,
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520,
Department of Chemistry, Boston College, Chestnut Hill, MA 02467,
Department of Physics, City College of New York, New York, NY 100031,
Department of Biochemistry, University of California, Riverside, CA 92521
Department of Chemistry, Yale University, New Haven, CT 06520,, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520,

Significance Photosystem II (PSII) is a photo-oxidoreductase that harnesses light energy to use water to make fuel. Water oxidation occurs at a metal cluster in the active site called the oxygen-evolving complex (OEC). Understanding PSII function has provided design principles for synthetic solar fuel catalysts; however, the details of water oxidation are obscured by the multiple states through which the mechanism proceeds, differences between species, and lability of the OEC. To better understand PSII function, we solved its structure from Synechocystis sp. PCC 6803. We observe significant differences compared with PSII from thermophilic cyanobacteria that highlight the need for reexamination of previous data using this structure for interpretation. The structure also provides a platform for studies of site-directed mutations of PSII.

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Research Organization:: Yale Univ., New Haven, CT (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-05ER15646; SC0001423; SC0005291

OSTI ID:: 1837119

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 1 Vol. 119; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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Title: High-resolution cryo-electron microscopy structure of photosystem II from the mesophilic cyanobacterium, Synechocystis sp. PCC 6803

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