Interdependent iron and phosphorus availability controls photosynthesis through retrograde signaling

Nam, Hye-In; Shahzad, Zaigham; Dorone, Yanniv; Clowez, Sophie; Zhao, Kangmei; Bouain, Nadia; Lay-Pruitt, Katerina S.; Cho, Huikyong; Rhee, Seung Y.; Rouached, Hatem

doi:10.1038/s41467-021-27548-2

Title: Interdependent iron and phosphorus availability controls photosynthesis through retrograde signaling

Journal Article · 09 December 2021 · Nature Communications

DOI: https://doi.org/10.1038/s41467-021-27548-2 · OSTI ID:1835175

Nam, Hye-In; Shahzad, Zaigham; Dorone, Yanniv; Clowez, Sophie;

; Bouain, Nadia;

; Cho, Huikyong;

;

Abstract Iron deficiency hampers photosynthesis and is associated with chlorosis. We recently showed that iron deficiency-induced chlorosis depends on phosphorus availability. How plants integrate these cues to control chlorophyll accumulation is unknown. Here, we show that iron limitation downregulates photosynthesis genes in a phosphorus-dependent manner. Using transcriptomics and genome-wide association analysis, we identify two genes, PHT4;4 encoding a chloroplastic ascorbate transporter and bZIP58 , encoding a nuclear transcription factor, which prevent the downregulation of photosynthesis genes leading to the stay-green phenotype under iron-phosphorus deficiency. Joint limitation of these nutrients induces ascorbate accumulation by activating expression of an ascorbate biosynthesis gene, VTC4 , which requires bZIP58. Furthermore, we demonstrate that chloroplastic ascorbate transport prevents the downregulation of photosynthesis genes under iron-phosphorus combined deficiency through modulation of ROS homeostasis. Our study uncovers a ROS-mediated chloroplastic retrograde signaling pathway to adapt photosynthesis to nutrient availability.

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Research Organization:: Carnegie Inst. of Washington, Washington, DC (United States); Donald Danforth Plant Science Center, St. Louis, MO (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0008769; SC0018277; SC0020366

OSTI ID:: 1835175

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 12; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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