Development and evaluation of an agar capture system (ACS) for high-throughput screening of insoluble particulate substrates with bacterial growth and enzyme activity assays
Abstract
In this work, we describe a method for containing insoluble particulates for use as substrates in either bacterial growth or enzyme assays. This method was designed for high-throughput screening of environmental or engineered bacteria. Benchmarking this method with several model bacteria uncovered phenotypes not observable with the particulate substrates alone.
- Authors:
-
- Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States)
- Publication Date:
- Research Org.:
- Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)
- OSTI Identifier:
- 1834060
- Alternate Identifier(s):
- OSTI ID: 1823485
- Grant/Contract Number:
- SC0014183; T32-GM066706
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Microbiological Methods
- Additional Journal Information:
- Journal Volume: 190; Journal ID: ISSN 0167-7012
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Biomass containment device; Carbohydrate active enzyme; Cellulose; High-throughput screening; Polysaccharide degradation; Renewable fuels and chemicals
Citation Formats
Garcia, Cecelia A., and Gardner, Jeffrey G. Development and evaluation of an agar capture system (ACS) for high-throughput screening of insoluble particulate substrates with bacterial growth and enzyme activity assays. United States: N. p., 2021.
Web. doi:10.1016/j.mimet.2021.106337.
Garcia, Cecelia A., & Gardner, Jeffrey G. Development and evaluation of an agar capture system (ACS) for high-throughput screening of insoluble particulate substrates with bacterial growth and enzyme activity assays. United States. https://doi.org/10.1016/j.mimet.2021.106337
Garcia, Cecelia A., and Gardner, Jeffrey G. Fri .
"Development and evaluation of an agar capture system (ACS) for high-throughput screening of insoluble particulate substrates with bacterial growth and enzyme activity assays". United States. https://doi.org/10.1016/j.mimet.2021.106337. https://www.osti.gov/servlets/purl/1834060.
@article{osti_1834060,
title = {Development and evaluation of an agar capture system (ACS) for high-throughput screening of insoluble particulate substrates with bacterial growth and enzyme activity assays},
author = {Garcia, Cecelia A. and Gardner, Jeffrey G.},
abstractNote = {In this work, we describe a method for containing insoluble particulates for use as substrates in either bacterial growth or enzyme assays. This method was designed for high-throughput screening of environmental or engineered bacteria. Benchmarking this method with several model bacteria uncovered phenotypes not observable with the particulate substrates alone.},
doi = {10.1016/j.mimet.2021.106337},
journal = {Journal of Microbiological Methods},
number = ,
volume = 190,
place = {United States},
year = {Fri Sep 24 00:00:00 EDT 2021},
month = {Fri Sep 24 00:00:00 EDT 2021}
}
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