Accelerating strain phenotyping with desorption electrospray ionization-imaging mass spectrometry and untargeted analysis of intact microbial colonies
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- Department of Chemistry, Vanderbilt University, Nashville, TN 37235,, Center for Innovative Technology, Vanderbilt University, Nashville, TN 37235,, Institute of Chemical Biology, Vanderbilt University, Nashville, TN 37235,, Institute for Integrative Biosystems Research and Education, Vanderbilt University, Nashville, TN 37235,, Vanderbilt-Ingram Cancer Center, Vanderbilt University, Nashville, TN 37232,
- Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN 37235,
- Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232,, Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232,
- Department of Chemical and Biological Engineering, University of Wisconsin, Madison, WI 53706
- Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN 37235,, Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232,
Significance Synthetic biology has entered an era in which reading and writing DNA sequences are no longer rate-limiting steps in microbial strain engineering. Indeed, analytical methods measuring the resulting metabolic outcomes of specific gene edits have lagged behind the ability to generate new recombinant strains. Herein, we report a mass spectrometry strategy to accelerate these analytical workflows by directly analyzing metabolites and molecules produced from engineered microorganisms in a multiplexed process. Using untargeted acquisitions and unsupervised analytics, we assess the molecular features that change across discrete strains including primary target species, secondary products, and species outside the engineered fatty acid biosynthesis pathway.
- Research Organization:
- Vanderbilt Univ., Nashville, TN (United States)
- Sponsoring Organization:
- USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)
- Grant/Contract Number:
- SC0019404; SC0022207
- OSTI ID:
- 1833748
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 49 Vol. 118; ISSN 0027-8424
- Publisher:
- Proceedings of the National Academy of SciencesCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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