An integrated host-microbiome response to atrazine exposure mediates toxicity in Drosophila

Brown, James B.; Langley, Sasha A.; Snijders, Antoine M.; Wan, Kenneth H.; Morris, Siti Nur Sarah; Booth, Benjamin W.; Fisher, William W.; Hammonds, Ann S.; Park, Soo; Weiszmann, Richard; Yu, Charles; Kirwan, Jennifer A.; Weber, Ralf J. M.; Viant, Mark R.; Mao, Jian-Hua; Celniker, Susan E.

doi:10.1038/s42003-021-02847-y

Title: An integrated host-microbiome response to atrazine exposure mediates toxicity in Drosophila

Journal Article · 24 November 2021 · Communications Biology

DOI: https://doi.org/10.1038/s42003-021-02847-y · OSTI ID:1832512

; Langley, Sasha A.;

;

; Morris, Siti Nur Sarah; Booth, Benjamin W.; Fisher, William W.; Hammonds, Ann S.; Park, Soo; Weiszmann, Richard; Yu, Charles;

;

Abstract The gut microbiome produces vitamins, nutrients, and neurotransmitters, and helps to modulate the host immune system—and also plays a major role in the metabolism of many exogenous compounds, including drugs and chemical toxicants. However, the extent to which specific microbial species or communities modulate hazard upon exposure to chemicals remains largely opaque. Focusing on the effects of collateral dietary exposure to the widely used herbicide atrazine, we applied integrated omics and phenotypic screening to assess the role of the gut microbiome in modulating host resilience in Drosophila melanogaster . Transcriptional and metabolic responses to these compounds are sex-specific and depend strongly on the presence of the commensal microbiome. Sequencing the genomes of all abundant microbes in the fly gut revealed an enzymatic pathway responsible for atrazine detoxification unique to Acetobacter tropicalis . We find that Acetobacter tropicalis alone, in gnotobiotic animals, is sufficient to rescue increased atrazine toxicity to wild-type, conventionally reared levels. This work points toward the derivation of biotic strategies to improve host resilience to environmental chemical exposures, and illustrates the power of integrative omics to identify pathways responsible for adverse health outcomes.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1832512

Journal Information:: Communications Biology, Journal Name: Communications Biology Journal Issue: 1 Vol. 4; ISSN 2399-3642

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Title: An integrated host-microbiome response to atrazine exposure mediates toxicity in Drosophila

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