Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling

Barnett, Samuel E.; Youngblut, Nicholas D.; Koechli, Chantal N.; Buckley, Daniel H.

doi:10.1073/pnas.2115292118

Title: Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling

Journal Article · 18 November 2021 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.2115292118 · OSTI ID:1831533

^[1]; Youngblut, Nicholas D. ^[2]; Koechli, Chantal N. ^[3];

^[1]

School of Integrative Plant Science, Cornell University, Ithaca, NY 14853,
School of Integrative Plant Science, Cornell University, Ithaca, NY 14853,, Department of Microbiome Science, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany,
School of Integrative Plant Science, Cornell University, Ithaca, NY 14853,, Department of Biological Sciences, University of the Sciences, Philadelphia, PA 19104

Significance Soil organic matter contains a large fraction of global C whose fate is largely governed by soil microbial communities. Global C-cycle models can be improved using information about microbial processes in soil, but the inner workings of soil communities remain largely hidden from view. We used stable isotopes to characterize the guild structure of soil bacteria based on how they assimilated C from different sources. Guild structure corresponded with growth dynamics and rrn copy number. We also show that this guild structure explains microbial biogeographical patterns at continental and global scales. These results suggest that microbial contributions to soil C cycling are largely governed by an anabolic process that governs energy allocation to growth, competition, and survival.

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Research Organization:: Cornell Univ., Ithaca, NY (United States); Cornell University, Ithaca, NY (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science (BSS)

Grant/Contract Number:: SC0004486; SC0010558; SC0016364

OSTI ID:: 1831533

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 47 Vol. 118; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling

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