Improved Surface Functionalization and Characterization of Membrane-Targeted Semiconductor Voltage Nanosensors

Park, Joonhyuck; Kuo, Yung; Li, Jack; Huang, Yi-Lin; Miller, Evan W.; Weiss, Shimon

doi:10.1021/acs.jpclett.9b01258

Title: Improved Surface Functionalization and Characterization of Membrane-Targeted Semiconductor Voltage Nanosensors

Journal Article · 26 June 2019 · Journal of Physical Chemistry Letters

DOI: https://doi.org/10.1021/acs.jpclett.9b01258 · OSTI ID:1831520

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^[1]; Li, Jack ^[1]; Huang, Yi-Lin ^[2];

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Univ. of California, Los Angeles, CA (United States)
Univ. of California, Berkeley, CA (United States)
Univ. of California, Los Angeles, CA (United States); Bar-Ilan Univ., Ramat Gan (Israel)

In this work, type-II ZnSe/CdS voltage sensing seeded nanorods (vsNRs) were functionalized with alpha helical peptides and zwitterionic decorated lipoic acids (zw LA). Specific membrane targeting with high loading efficiency and minimal non specific binding was achieved . These vsNRs display quantum yield (QY) modulation as function of membrane potential (MP) changes, as demonstrated at the ensemble level for (i) vesicles treated with valinomycin and (ii) wild type HEK cells under alternating buffers with different [K⁺]. ΔF/F of ~ 1% was achieved.

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Research Organization:: Univ. of California, Los Angeles, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); United States–Israel Binational Science Foundation; Human Frontier Science Program; Defense Advanced Research Projects Agency (DARPA); European Research Council (ERC); National Science Foundation (NSF)

Grant/Contract Number:: FC03-02ER63421; AC02-05CH11231; 1S10RR23057; GM071940; 2010382; RGP0061/2015; D14PC00141; 669941; DMR 1548924

OSTI ID:: 1831520

Journal Information:: Journal of Physical Chemistry Letters, Vol. 10, Issue 14; ISSN 1948-7185

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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All-Optical Detection of Neuronal Membrane Depolarization in Live Cells Using Colloidal Quantum Dots Caglar, Mustafa; Pandya, Raj; Xiao, James Nano Letters, Vol. 19, Issue 12 https://doi.org/10.1021/acs.nanolett.9b03026	journal	November 2019

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