Bacterial response to spatial gradients of algal-derived nutrients in a porous microplate

Kim, Hyungseok; Kimbrel, Jeffrey A.; Vaiana, Christopher A.; Wollard, Jessica R.; Mayali, Xavier; Buie, Cullen R.

doi:10.1038/s41396-021-01147-x

Title: Bacterial response to spatial gradients of algal-derived nutrients in a porous microplate

Journal Article · 17 November 2021 · The ISME Journal

DOI: https://doi.org/10.1038/s41396-021-01147-x · OSTI ID:1830852

^[1];

^[2]; Vaiana, Christopher A. ^[1];

^[2];

^[2]; Buie, Cullen R. ^[1]

Massachusetts Institute of Technology (MIT), Cambridge, MA (United States)
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Photosynthetic microalgae are responsible for 50% of the global atmospheric CO2 fixation into organic matter and hold potential as a renewable bioenergy source. Their metabolic interactions with the surrounding microbial community (the algal microbiome) play critical roles in carbon cycling, but due to methodological limitations, it has been challenging to examine how community development is influenced by spatial proximity to their algal host. Here we introduce a copolymer-based porous microplate to co-culture algae and bacteria, where metabolites are constantly exchanged between the microorganisms while maintaining physical separation. In the microplate, we found that the diatom Phaeodactylum tricornutum accumulated to cell abundances ~20 fold higher than under normal batch conditions due to constant replenishment of nutrients through the porous structure. We also demonstrate that algal-associated bacteria, both single isolates and complex communities, responded to inorganic nutrients away from their host as well as organic nutrients originating from the algae in a spatially predictable manner. These experimental findings coupled with a mathematical model suggest that host proximity and algal culture growth phase impact bacterial community development in a taxon-specific manner through organic and inorganic nutrient availability. Our novel system presents a useful tool to investigate universal metabolic interactions between microbes in aquatic ecosystems.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE; USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1830852

Report Number(s):: LLNL--JRNL-817555

Journal Information:: The ISME Journal, Journal Name: The ISME Journal Journal Issue: 4 Vol. 16; ISSN 1751-7370; ISSN 1751-7362

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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