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Title: Bacterial response to spatial gradients of algal-derived nutrients in a porous microplate

Abstract

Abstract Photosynthetic microalgae are responsible for 50% of the global atmospheric CO2 fixation into organic matter and hold potential as a renewable bioenergy source. Their metabolic interactions with the surrounding microbial community (the algal microbiome) play critical roles in carbon cycling, but due to methodological limitations, it has been challenging to examine how community development is influenced by spatial proximity to their algal host. Here we introduce a copolymer-based porous microplate to co-culture algae and bacteria, where metabolites are constantly exchanged between the microorganisms while maintaining physical separation. In the microplate, we found that the diatom Phaeodactylum tricornutum accumulated to cell abundances ~20 fold higher than under normal batch conditions due to constant replenishment of nutrients through the porous structure. We also demonstrate that algal-associated bacteria, both single isolates and complex communities, responded to inorganic nutrients away from their host as well as organic nutrients originating from the algae in a spatially predictable manner. These experimental findings coupled with a mathematical model suggest that host proximity and algal culture growth phase impact bacterial community development in a taxon-specific manner through organic and inorganic nutrient availability. Our novel system presents a useful tool to investigate universal metabolic interactions between microbes inmore » aquatic ecosystems.« less

Authors:
ORCiD logo; ORCiD logo; ; ORCiD logo; ORCiD logo;
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1830852
Resource Type:
Published Article
Journal Name:
The ISME Journal
Additional Journal Information:
Journal Name: The ISME Journal Journal Volume: 16 Journal Issue: 4; Journal ID: ISSN 1751-7362
Publisher:
Oxford University Press
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Kim, Hyungseok, Kimbrel, Jeffrey A., Vaiana, Christopher A., Wollard, Jessica R., Mayali, Xavier, and Buie, Cullen R. Bacterial response to spatial gradients of algal-derived nutrients in a porous microplate. United Kingdom: N. p., 2021. Web. doi:10.1038/s41396-021-01147-x.
Kim, Hyungseok, Kimbrel, Jeffrey A., Vaiana, Christopher A., Wollard, Jessica R., Mayali, Xavier, & Buie, Cullen R. Bacterial response to spatial gradients of algal-derived nutrients in a porous microplate. United Kingdom. https://doi.org/10.1038/s41396-021-01147-x
Kim, Hyungseok, Kimbrel, Jeffrey A., Vaiana, Christopher A., Wollard, Jessica R., Mayali, Xavier, and Buie, Cullen R. Wed . "Bacterial response to spatial gradients of algal-derived nutrients in a porous microplate". United Kingdom. https://doi.org/10.1038/s41396-021-01147-x.
@article{osti_1830852,
title = {Bacterial response to spatial gradients of algal-derived nutrients in a porous microplate},
author = {Kim, Hyungseok and Kimbrel, Jeffrey A. and Vaiana, Christopher A. and Wollard, Jessica R. and Mayali, Xavier and Buie, Cullen R.},
abstractNote = {Abstract Photosynthetic microalgae are responsible for 50% of the global atmospheric CO2 fixation into organic matter and hold potential as a renewable bioenergy source. Their metabolic interactions with the surrounding microbial community (the algal microbiome) play critical roles in carbon cycling, but due to methodological limitations, it has been challenging to examine how community development is influenced by spatial proximity to their algal host. Here we introduce a copolymer-based porous microplate to co-culture algae and bacteria, where metabolites are constantly exchanged between the microorganisms while maintaining physical separation. In the microplate, we found that the diatom Phaeodactylum tricornutum accumulated to cell abundances ~20 fold higher than under normal batch conditions due to constant replenishment of nutrients through the porous structure. We also demonstrate that algal-associated bacteria, both single isolates and complex communities, responded to inorganic nutrients away from their host as well as organic nutrients originating from the algae in a spatially predictable manner. These experimental findings coupled with a mathematical model suggest that host proximity and algal culture growth phase impact bacterial community development in a taxon-specific manner through organic and inorganic nutrient availability. Our novel system presents a useful tool to investigate universal metabolic interactions between microbes in aquatic ecosystems.},
doi = {10.1038/s41396-021-01147-x},
journal = {The ISME Journal},
number = 4,
volume = 16,
place = {United Kingdom},
year = {Wed Nov 17 00:00:00 EST 2021},
month = {Wed Nov 17 00:00:00 EST 2021}
}

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