Sulfate adenylyl transferase kinetics and mechanisms of metabolic inhibitors of microbial sulfate respiration
Abstract
Abstract Sulfate analog oxyanions that function as selective metabolic inhibitors of dissimilatory sulfate reducing microorganisms (SRM) are widely used in ecological studies and industrial applications. As such, it is important to understand the mode of action and mechanisms of tolerance or adaptation to these compounds. Different oxyanions vary widely in their inhibitory potency and mechanism of inhibition, but current evidence suggests that the sulfate adenylyl transferase/ATP sulfurylase (Sat) enzyme is an important target. We heterologously expressed and purified the Sat from the model SRM, Desulfovibrio alaskensis G20. With this enzyme we determined the turnover kinetics (kcat, KM) for alternative substrates (molybdate, selenate, arsenate, monofluorophosphate, and chromate) and inhibition constants (KI) for competitive inhibitors (perchlorate, chlorate, and nitrate). These measurements enable the first quantitative comparisons of these compounds as substrates or inhibitors of a purified Sat from a respiratory sulfate reducer. We compare predicted half-maximal inhibitory concentrations (IC50) based on Sat kinetics with measured IC50 values against D. alaskensis G20 growth and discuss our results in light of known mechanisms of sensitivity or resistance to oxyanions. This analysis helps with the interpretation of recent adaptive laboratory evolution studies and illustrates the value of interpreting gene–microbe–environment interactions through the lens of enzymemore »
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1830237
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Published Article
- Journal Name:
- ISME Communications
- Additional Journal Information:
- Journal Name: ISME Communications Journal Volume: 1 Journal Issue: 1; Journal ID: ISSN 2730-6151
- Publisher:
- Oxford University Press
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Carlson, Hans K., Youngblut, Matthew D., Redford, Steven A., Williamson, Adam J., and Coates, John D. Sulfate adenylyl transferase kinetics and mechanisms of metabolic inhibitors of microbial sulfate respiration. United States: N. p., 2021.
Web. doi:10.1038/s43705-021-00069-1.
Carlson, Hans K., Youngblut, Matthew D., Redford, Steven A., Williamson, Adam J., & Coates, John D. Sulfate adenylyl transferase kinetics and mechanisms of metabolic inhibitors of microbial sulfate respiration. United States. https://doi.org/10.1038/s43705-021-00069-1
Carlson, Hans K., Youngblut, Matthew D., Redford, Steven A., Williamson, Adam J., and Coates, John D. Sat .
"Sulfate adenylyl transferase kinetics and mechanisms of metabolic inhibitors of microbial sulfate respiration". United States. https://doi.org/10.1038/s43705-021-00069-1.
@article{osti_1830237,
title = {Sulfate adenylyl transferase kinetics and mechanisms of metabolic inhibitors of microbial sulfate respiration},
author = {Carlson, Hans K. and Youngblut, Matthew D. and Redford, Steven A. and Williamson, Adam J. and Coates, John D.},
abstractNote = {Abstract Sulfate analog oxyanions that function as selective metabolic inhibitors of dissimilatory sulfate reducing microorganisms (SRM) are widely used in ecological studies and industrial applications. As such, it is important to understand the mode of action and mechanisms of tolerance or adaptation to these compounds. Different oxyanions vary widely in their inhibitory potency and mechanism of inhibition, but current evidence suggests that the sulfate adenylyl transferase/ATP sulfurylase (Sat) enzyme is an important target. We heterologously expressed and purified the Sat from the model SRM, Desulfovibrio alaskensis G20. With this enzyme we determined the turnover kinetics (kcat, KM) for alternative substrates (molybdate, selenate, arsenate, monofluorophosphate, and chromate) and inhibition constants (KI) for competitive inhibitors (perchlorate, chlorate, and nitrate). These measurements enable the first quantitative comparisons of these compounds as substrates or inhibitors of a purified Sat from a respiratory sulfate reducer. We compare predicted half-maximal inhibitory concentrations (IC50) based on Sat kinetics with measured IC50 values against D. alaskensis G20 growth and discuss our results in light of known mechanisms of sensitivity or resistance to oxyanions. This analysis helps with the interpretation of recent adaptive laboratory evolution studies and illustrates the value of interpreting gene–microbe–environment interactions through the lens of enzyme kinetics.},
doi = {10.1038/s43705-021-00069-1},
journal = {ISME Communications},
number = 1,
volume = 1,
place = {United States},
year = {Sat Nov 13 00:00:00 EST 2021},
month = {Sat Nov 13 00:00:00 EST 2021}
}
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