Title: Effect of Active Lengthening and Shortening on Small-Angle X-ray Reflections in Skinned Skeletal Muscle Fibres

Journal Article · · International Journal of Molecular Sciences (Online)
 [1]; ORCiD logo [1]; ORCiD logo [2];  [1]; ORCiD logo [3];  [4]; ORCiD logo [3];  [1]
  1. Univ. of Calgary, AB (Canada). Human Performance Lab. Faculty of Kinesiology. McCaig Inst. for Bone and Joint Health
  2. Ritsumeikan Univ., Kusatsu (Japan). Faculty of Sport and Health Science
  3. Illinois Institute of Technology, Chicago, IL (United States). The Biophysics Collaborative Access Team (BioCAT). Dept. of Biological Sciences
  4. Illinois Institute of Technology, Chicago, IL (United States). Dept. of Biological Sciences
+ Show Author Affiliations

Our purpose was to use small-angle X-ray diffraction to investigate the structural changes within sarcomeres at steady-state isometric contraction following active lengthening and shortening, compared to purely isometric contractions performed at the same final lengths. We examined force, stiffness, and the 1,0 and 1,1 equatorial and M3 and M6 meridional reflections in skinned rabbit psoas bundles, at steady-state isometric contraction following active lengthening to a sarcomere length of 3.0 µm (15.4% initial bundle length at 7.7% bundle length/s), and active shortening to a sarcomere length of 2.6 µm (15.4% bundle length at 7.7% bundle length/s), and during purely isometric reference contractions at the corresponding sarcomere lengths. Compared to the reference contraction, the isometric contraction after active lengthening was associated with an increase in force (i.e., residual force enhancement) and M3 spacing, no change in stiffness and the intensity ratio I1,1/I1,0, and decreased lattice spacing and M3 intensity. Compared to the reference contraction, the isometric contraction after active shortening resulted in decreased force, stiffness, I1,1/I1,0, M3 and M6 spacings, and M3 intensity. This suggests that residual force enhancement is achieved without an increase in the proportion of attached cross-bridges, and that force depression is accompanied by a decrease in the proportion of attached cross-bridges. Furthermore, the steady-state isometric contraction following active lengthening and shortening is accompanied by an increase in cross-bridge dispersion and/or a change in the cross-bridge conformation compared to the reference contractions.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC02-06CH11357
OSTI ID:
1826791
Journal Information:
International Journal of Molecular Sciences (Online), Journal Name: International Journal of Molecular Sciences (Online) Journal Issue: 16 Vol. 22; ISSN 1422-0067
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
ENGLISH

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
equatorial X-ray reflections
force depression
lattice spacing
meridional X-ray reflections
residual force enhancement
shift in mass
stiffness