Widespread bacterial diversity within the bacteriome of fungi

Robinson, Aaron J.; House, Geoffrey L.; Morales, Demosthenes P.; Kelliher, Julia M.; Gallegos-Graves, La Verne; LeBrun, Erick S.; Davenport, Karen W.; Palmieri, Fabio; Lohberger, Andrea; Bregnard, Danaé; Estoppey, Aislinn; Buffi, Matteo; Paul, Christophe; Junier, Thomas; Hervé, Vincent; Cailleau, Guillaume; Lupini, Simone; Nguyen, Hang N.; Zheng, Amy O.; Gimenes, Luciana Jandelli; Bindschedller, Saskia; Rodrigues, Debora F.; Werner, James H.; Young, Jamey D.; Junier, Pilar; Chain, Patrick S. G.

doi:10.1038/s42003-021-02693-y

Title: Widespread bacterial diversity within the bacteriome of fungi

Journal Article · 07 October 2021 · Communications Biology

DOI: https://doi.org/10.1038/s42003-021-02693-y · OSTI ID:1824864

; House, Geoffrey L.; Morales, Demosthenes P.;

; Gallegos-Graves, La Verne;

; Davenport, Karen W.;

; Lohberger, Andrea;

; Estoppey, Aislinn; Buffi, Matteo;

; Junier, Thomas;

;

; Lupini, Simone;

; Zheng, Amy O.; Gimenes, Luciana Jandelli more »

Abstract Knowledge of associations between fungal hosts and their bacterial associates has steadily grown in recent years as the number and diversity of examinations have increased, but current knowledge is predominantly limited to a small number of fungal taxa and bacterial partners. Here, we screened for potential bacterial associates in over 700 phylogenetically diverse fungal isolates, representing 366 genera, or a tenfold increase compared with previously examined fungal genera, including isolates from several previously unexplored phyla. Both a 16 S rDNA-based exploration of fungal isolates from four distinct culture collections spanning North America, South America and Europe, and a bioinformatic screen for bacterial-specific sequences within fungal genome sequencing projects, revealed that a surprisingly diverse array of bacterial associates are frequently found in otherwise axenic fungal cultures. We demonstrate that bacterial associations with diverse fungal hosts appear to be the rule, rather than the exception, and deserve increased consideration in microbiome studies and in examinations of microbial interactions.

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Sponsoring Organization:: USDOE

OSTI ID:: 1824864

Journal Information:: Communications Biology, Journal Name: Communications Biology Journal Issue: 1 Vol. 4; ISSN 2399-3642

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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