Rhoptry kinase protein 39 (ROP39) is a novel factor that recruits host mitochondria to the parasitophorous vacuole of Toxoplasma gondii

Fukumoto, Junpei; Sakura, Takaya; Matsubara, Ryuma; Tahara, Michiru; Matsuzaki, Motomichi; Nagamune, Kisaburo

doi:10.1242/bio.058988

Title: Rhoptry kinase protein 39 (ROP39) is a novel factor that recruits host mitochondria to the parasitophorous vacuole of Toxoplasma gondii

Journal Article · 30 September 2021 · Biology Open

DOI: https://doi.org/10.1242/bio.058988 · OSTI ID:1823177

Fukumoto, Junpei ^[1]; Sakura, Takaya ^[2]; Matsubara, Ryuma ^[1]; Tahara, Michiru ^[2]; Matsuzaki, Motomichi ^[2];

^[3]

Department of Parasitology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan
Department of Parasitology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan
Department of Parasitology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan, Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan

ABSTRACT Most intracellular pathogens replicate in a vacuole to avoid the defense system of the host. A few pathogens recruit host mitochondria around those vacuoles, but the molecules responsible for mitochondrial recruitment remain unidentified. It is only in the apicomplexan parasite Toxoplasma gondii, that mitochondrial association factor 1b (MAF1b) has been identified as an association factor for host mitochondria. Here, we show that rhoptry kinase family protein 39 (ROP39) induces host mitochondrial recruitment in T. gondii. We found that the abundance of ROP39 was increased on host mitochondria extracted from human foreskin fibroblasts (HFFs) infected with T. gondii. ROP39 expressed exogenously in HFFs localized on host mitochondria, indicating that it has the potential to bind to host mitochondria without assistance from other parasite factors. Confocal microscopy revealed that ROP39 colocalized with host mitochondria on the membrane of parasitophorous vacuoles, in which the parasites reside. Moreover, we observed about a 10% reduction in the level of mitochondrial association in rop39-knockout parasites compared with a parental strain.

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Sponsoring Organization:: USDOE

OSTI ID:: 1823177

Journal Information:: Biology Open, Journal Name: Biology Open Journal Issue: 9 Vol. 10; ISSN 2046-6390

Publisher:: The Company of BiologistsCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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