Paramagnetic Mn8Fe4-co-Polystyrene Nanobeads as a Potential T1–T2 Multimodal Magnetic Resonance Imaging Contrast Agent with In Vivo Studies

Dahanayake, Vidumin; Lyons, Trevor; Kerwin, Brendan; Rodriguez, Olga; Albanese, Christopher; Parasido, Erika; Lee, Yichien; Keuren, Edward Van; Li, Luxi; Maxey, Evan; Paunesku, Tatjana; Woloschak, Gayle; Stoll, Sarah L.

doi:10.1021/acsami.1c09232

Title: Paramagnetic Mn₈Fe₄-co-Polystyrene Nanobeads as a Potential T₁–T₂ Multimodal Magnetic Resonance Imaging Contrast Agent with In Vivo Studies

Journal Article · 10 August 2021 · ACS Applied Materials and Interfaces

DOI: https://doi.org/10.1021/acsami.1c09232 · OSTI ID:1822168

Dahanayake, Vidumin ^[1]; Lyons, Trevor ^[2]; Kerwin, Brendan ^[1]; Rodriguez, Olga ^[2]; Albanese, Christopher ^[2]; Parasido, Erika ^[2]; Lee, Yichien ^[2]; Keuren, Edward Van ^[1]; Li, Luxi ^[3]; Maxey, Evan ^[3]; Paunesku, Tatjana ^[4];

^[4];

^[1]

Georgetown Univ., Washington, DC (United States)
Georgetown Univ., Washington, DC (United States). Medical Center
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Northwestern Univ., Chicago, IL (United States)

In developing a cluster-nanocarrier design, as a magnetic resonance imaging contrast agent, we have investigated the enhanced relaxivity of a manganese and iron-oxo cluster grafted within a porous polystyrene nanobead with increased relaxivity due to a higher surface area. The synthesis of the cluster-nanocarrier for the cluster Mn₈Fe₄O₁₂(O₂CC₆H₄CH=CH₂)₁₆(H₂O)₄, cross-linked with polystyrene (the nano-carrier), under miniemulsion conditions is described. By including a branched hydrophobe, iso-octane, the resulting nanobeads are porous and similar to 70 nm in diameter. The increased surface area of the nanobeads compared to nonporous nanobeads leads to an enhancement in relaxivity; r₁ increases from 3.8 to 5.2 ± 0.1 mM^-1 s^-1, and r₂ increases from 11.9 to 50.1 ± 4.8 mM^-1 s^-1, at 9.4 teslas, strengthening the potential for T₁ and T₂ imaging. Several metrics were used to assess stability, and the porosity produced no reduction in metal stability. Synchrotron X-ray fluorescence microscopy was used to demonstrate that the nanobeads remain intact in vivo. In depth, physicochemical characteristics were determined, including extensive pharmacokinetics, in vivo imaging, and systemic biodistribution analysis.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1822168

Journal Information:: ACS Applied Materials and Interfaces, Journal Name: ACS Applied Materials and Interfaces Journal Issue: 33 Vol. 13; ISSN 1944-8244

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
MRI contrast agent
XRF microscopy
biocompatible
cluster-nanocarrier
miniemulsion

Title: Paramagnetic Mn8Fe4-co-Polystyrene Nanobeads as a Potential T1–T2 Multimodal Magnetic Resonance Imaging Contrast Agent with In Vivo Studies

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Title: Paramagnetic Mn₈Fe₄-co-Polystyrene Nanobeads as a Potential T₁–T₂ Multimodal Magnetic Resonance Imaging Contrast Agent with In Vivo Studies