Biochemical inhibition of acid phosphatase activity in two mountain spruce forest soils

Čapek, Petr; Kasanke, Christopher P.; Starke, Robert; Zhao, Qian; Tahovská, Karolina

doi:10.1007/s00374-021-01587-9

Title: Biochemical inhibition of acid phosphatase activity in two mountain spruce forest soils

Journal Article · 23 August 2021 · Biology and Fertility of Soils

DOI: https://doi.org/10.1007/s00374-021-01587-9 · OSTI ID:1821861

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Univ. of South Bohemia, České Budějovice (Czech Republic)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Czech Academy of Sciences, Praha (Czech Republic). Inst. of Microbiology

The product inhibition of the 4-Methylumbelliferyl phosphate (MUB-P) decay rate, a measure of potential acid phosphatase activity, has not been considered in most of the published kinetic studies. The aim of this study was to determine the type and strength of the product inhibition in order to better define reaction conditions at which the acid phosphatase activity assay produces unbiased results. The MUB-P decay rate was measured in the forest floor and topsoil organic horizons of two spruce forest catchments at different initial MUB-P and P-PO4 3- concentrations. Furthermore, the type and strength of the inhibition was analyzed by non-linear regression. We found that MUB-P decay was competitively inhibited by the P-PO4 3- but also by dissolved organic P. By using estimated kinetic parameters, we calculated an underestimation of potential acid phosphatase activity by up to ~20% at concentrations of added MUB-P as high as 300 µmol g-1 dry weight and an incubation time one hour due to background inorganic and organic P concentrations in range of units of µmol per gram of dry soil. We discuss approaches that can be used to minimize the underestimation and show that the previously defined recommendations might not be always sufficient to avoid the bias. Therefore, we recommend to analyze progress curves at a wide range of initial MUB-P concentrations using non-linear methods when the enzyme assay is optimized for a given soil. When changes in inorganic and/or organic P concentrations are expected, we further recommend measuring background inorganic and organic P concentrations.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: Czech Science Foundation; USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1821861

Report Number(s):: PNNL-SA--148397

Journal Information:: Biology and Fertility of Soils, Journal Name: Biology and Fertility of Soils Journal Issue: 7 Vol. 57; ISSN 0178-2762

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Michaelis-Menten kinetic
biochemical inhibition
enzyme assay
linear regression
soil acid phosphatases

Title: Biochemical inhibition of acid phosphatase activity in two mountain spruce forest soils

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