Sterols are required for the coordinated assembly of lipid droplets in developing seeds

Yu, Linhui; Fan, Jilian; Zhou, Chao; Xu, Changcheng

doi:10.1038/s41467-021-25908-6

Title: Sterols are required for the coordinated assembly of lipid droplets in developing seeds

Journal Article · 22 September 2021 · Nature Communications

DOI: https://doi.org/10.1038/s41467-021-25908-6 · OSTI ID:1821508

Yu, Linhui;

; Zhou, Chao;

Abstract Lipid droplets (LDs) are intracellular organelles critical for energy storage and lipid metabolism. They are typically composed of an oil core coated by a monolayer of phospholipids and proteins such as oleosins. The mechanistic details of LD biogenesis remain poorly defined. However, emerging evidence suggest that their formation is a spatiotemporally regulated process, occurring at specific sites of the endoplasmic reticulum defined by a specific set of lipids and proteins. Here, we show that sterols are required for formation of oleosin-coated LDs in Arabidopsis. Analysis of sterol pathway mutants revealed that deficiency in several ∆ ⁵ -sterols accounts for the phenotype. Importantly, mutants deficient in these sterols also display reduced LD number, increased LD size and reduced oil content in seeds. Collectively, our data reveal a role of sterols in coordinating the synthesis of oil and oleosins and their assembly into LDs, highlighting the importance of membrane lipids in regulating LD biogenesis.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0012704

OSTI ID:: 1821508

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 12; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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