Title: Aspects of the Neurospora crassa Sulfur Starvation Response Are Revealed by Transcriptional Profiling and DNA Affinity Purification Sequencing

Abstract

Accurate nutrient sensing is important for rapid fungal growth and exploitation of available resources. Sulfur is an important nutrient source found in a number of biological macromolecules, including proteins and lipids. The model filamentous fungus Neurospora crassa is capable of utilizing sulfur found in a variety of sources from amino acids to sulfate. During sulfur starvation, the transcription factor CYS-3 is responsible for upregulation of genes involved in sulfur uptake and assimilation. Using a combination of RNA sequencing and DNA affinity purification sequencing, we performed a global survey of the N. crassa sulfur starvation response and the role of CYS-3 in regulating sulfur-responsive genes. The CYS-3 transcription factor bound the promoters and regulated genes involved in sulfur metabolism. Additionally, CYS-3 directly activated the expression of a number of uncharacterized transporter genes, suggesting that regulation of sulfur import is an important aspect of regulation by CYS-3. CYS-3 also directly regulated the expression of genes involved in mitochondrial electron transfer. During sulfur starvation, genes involved in nitrogen metabolism, such as amino acid and nucleic acid metabolic pathways, along with genes encoding proteases and nucleases that are necessary for scavenging nitrogen, were activated. Sulfur starvation also caused changes in the expression of genesmore » involved in carbohydrate metabolism, such as those encoding glycosyl hydrolases. Thus, our data suggest a connection between sulfur metabolism and other aspects of cellular metabolism.« less

Authors:

Huberman, Lori B.  ^[1];

• Search DOE PAGES for author "Huberman, Lori B."
• Search DOE PAGES for ORCID "0000-0003-2638-8528"
• Search orcid.org for ORCID "0000-0003-2638-8528"

Wu, Vincent W. ^[2]; Lee, Juna ^[3]; Daum, Chris ^[3]; O’Malley, Ronan C. ^[4]; Glass, N. Louise  ^[5];

• Search DOE PAGES for author "Glass, N. Louise"
• Search DOE PAGES for ORCID "0000-0002-4844-2890"
• Search orcid.org for ORCID "0000-0002-4844-2890"

Mitchell, ed., Aaron P.

---

+ Show Author Affiliations

1. Plant and Microbial Biology Department, University of California, Berkeley, California, USA, Energy Biosciences Institute, University of California, Berkeley, California, USA, Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, New York, USA
2. Plant and Microbial Biology Department, University of California, Berkeley, California, USA, Energy Biosciences Institute, University of California, Berkeley, California, USA
3. U.S. Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USA
4. U.S. Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USA, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
5. Plant and Microbial Biology Department, University of California, Berkeley, California, USA, Energy Biosciences Institute, University of California, Berkeley, California, USA, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

Publication Date:

2021-10-27

Research Org.:

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE Office of Science (SC); USDOE Laboratory Directed Research and Development (LDRD) Program; National Institutes of Health (NIH)

OSTI Identifier:

1820496

Alternate Identifier(s):

OSTI ID: 1855186

Grant/Contract Number:  

AC02-05CH11231; ST32GM007127-39; CSP 982

Resource Type:

Published Article

Journal Name:

mSphere

Additional Journal Information:

Journal Name: mSphere Journal Volume: 6 Journal Issue: 5; Journal ID: ISSN 2379-5042

Publisher:

American Society for Microbiology

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; DAP-seq; nutrient sensing; sulfur starvation response; transcriptional profiling