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Title: A gain series method for accurate EMCCD calibration

Journal Article · · Scientific Reports
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Abstract Calibration of the gain and digital conversion factor of an EMCCD is necessary for accurate photon counting. We present a new method to quickly calibrate multiple gain settings of an EMCCD camera. Acquiring gain-series calibration data and analyzing the resulting images with the EMCCD noise model more accurately estimates the gain response of the camera. Furthermore, we develop a method to compare the results from different calibration approaches. Gain-series calibration outperforms all other methods in this self-consistency test.

Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Organization:
USDOE; USDOE Laboratory Directed Research and Development (LDRD) Program; USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science (BSS)
Grant/Contract Number:
89233218CNA000001
OSTI ID:
1820189
Report Number(s):
LA-UR--20-27551; LA-UR--21-24123; 18348; PII: 97759
Journal Information:
Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 11; ISSN 2045-2322
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United Kingdom
Language:
English

References (32)

Unraveling the Thousand Word Picture: An Introduction to Super-Resolution Data Analysis journal April 2017
Mapping Emission from Clusters of CdSe/ZnS Nanoparticles journal February 2018
Optimized measurements of separations and angles between intra-molecular fluorescent markers journal October 2015
Optimized localization analysis for single-molecule tracking and super-resolution microscopy journal April 2010
sCMOS noise-correction algorithm for microscopy images journal August 2017
Subunit counting in membrane-bound proteins journal March 2007
Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software journal April 2019
“Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images journal July 2016
Effects of fixed pattern noise on single molecule localization microscopy journal January 2014
CCD or CMOS camera noise characterisation journal November 2002
A framework for quantitative analysis of spectral data in two channels journal July 2020
Dissection of molecular assembly dynamics by tracking orientation and position of single molecules in live cells journal September 2016
Maximum-likelihood scintillation detection for EM-CCD based gamma cameras journal July 2011
Photon-number correlation for quantum enhanced imaging and sensing journal August 2017
Observation of spatial quantum correlations in the macroscopic regime journal May 2017
Excess noise and other important characteristics of low light level imaging using charge multiplying CCDs journal January 2003
The noise performance of electron multiplying charge-coupled devices journal May 2003
Statistics of single-electron signals in electron-multiplying charge-coupled devices journal April 2006
Analysis of electron multiplying charge coupled device and scientific CMOS readout noise models for Shack–Hartmann wavefront sensor accuracy journal July 2015
Methods for CCD camera characterization
  • Mullikin, James C.; van Vliet, Lucas J.; Netten, H.
  • IS&T/SPIE 1994 International Symposium on Electronic Imaging: Science and Technology, SPIE Proceedings https://doi.org/10.1117/12.175165
conference May 1994
A three-camera imaging microscope for high-speed single-molecule tracking and super-resolution imaging in living cells conference August 2015
EMCCD in-situ periodic characterization in Shack-Hartmann wavefront sensor for GTCAO conference July 2018
Electron-multiplying CCD: the new ICCD conference February 2003
Photon transfer methods and results for electron multiplication CCDs
  • DeWeert, Michael J.; Cole, Jeffrey B.; Sparks, Andrew W.
  • Optical Science and Technology, the SPIE 49th Annual Meeting, SPIE Proceedings https://doi.org/10.1117/12.562223
conference November 2004
Real-time quantification of single RNA translation dynamics in living cells journal May 2016
Assessing low-light cameras with photon transfer curve method journal May 2016
Fisher information theory for parameter estimation in single molecule microscopy: tutorial journal January 2016
Localization-based super-resolution microscopy with an sCMOS camera journal January 2011
Localization-based super-resolution microscopy with an sCMOS camera Part II: Experimental methodology for comparing sCMOS with EMCCD cameras journal January 2012
Algorithmic corrections for localization microscopy with sCMOS cameras - characterisation of a computationally efficient localization approach journal January 2017
Absolute calibration of an EMCCD camera by quantum correlation, linking photon counting to the analog regime journal January 2016
A Stochastic Model for Electron Multiplication Charge-Coupled Devices – From Theory to Practice journal January 2013

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36 MATERIALS SCIENCE
EMCCD
Material Science
calibration
camera
imaging and sensing
microscopy
single-molecule biophysics