Sorting for secreted molecule production using a biosensor-in-microdroplet approach

Bowman, Emily K.; Wagner, James M.; Yuan, Shuo-Fu; Deaner, Matthew; Palmer, Claire M.; D’Oelsnitz, Simon; Cordova, Lauren; Li, Xin; Craig, Frank F.; Alper, Hal S.

doi:10.1073/pnas.2106818118

Title: Sorting for secreted molecule production using a biosensor-in-microdroplet approach

Journal Article · 01 September 2021 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.2106818118 · OSTI ID:1819978

^[1]; Wagner, James M. ^[2]; Yuan, Shuo-Fu ^[1]; Deaner, Matthew ^[2];

^[1];

^[2]; Li, Xin ^[3]; Craig, Frank F. ^[3];

^[4]

Interdisciplinary Life Sciences Graduate Program, The University of Texas at Austin, Austin, TX 78712,
McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712,
Sphere Fluidics Limited, Cambridge CB21 6GP, United Kingdom
Interdisciplinary Life Sciences Graduate Program, The University of Texas at Austin, Austin, TX 78712,, McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712,

Significance The design–build–test cycle of metabolic engineering is swiftly becoming test limited owing to advances in DNA synthesis and library design. At the same time, improving extracellular production requires high-throughput studies to detect extracellular (not intracellular) content. Traditional approaches that utilize chromatography-based analysis or plate-based fluorescence assays are relatively throughput limited and require a high degree of liquid handling. These limitations are avoided here by creating a generalizable approach for small molecule production screening. Namely, this paradigm combines “off-the-shelf” biosensors with large library screening from producing cells in a cell-type agnostic manner.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1819978

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 36 Vol. 118; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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