Functional genetic encoding of sulfotyrosine in mammalian cells

He, Xinyuan; Chen, Yan; Beltran, Daisy Guiza; Kelly, Maia; Ma, Bin; Lawrie, Justin; Wang, Feng; Dodds, Eric; Zhang, Limei; Guo, Jiantao; Niu, Wei

doi:10.1038/s41467-020-18629-9

Title: Functional genetic encoding of sulfotyrosine in mammalian cells

Journal Article · 23 September 2020 · Nature Communications

DOI: https://doi.org/10.1038/s41467-020-18629-9 · OSTI ID:1817116

He, Xinyuan ^[1]; Chen, Yan ^[2]; Beltran, Daisy Guiza ^[3];

^[2]; Ma, Bin ^[2]; Lawrie, Justin ^[2];

^[4]; Dodds, Eric ^[2];

^[3];

^[2];

^[5]

Univ. of Nebraska, Lincoln, NE (United States). Dept. of Chemical & Biomolecular Engineering; OSTI
Univ. of Nebraska, Lincoln, NE (United States). Dept. of Chemistry
Univ. of Nebraska, Lincoln, NE (United States). Dept. of Biochemistry
Chinese Academy of Sciences (CAS), Beijing (China). Inst. of Biophysics
Univ. of Nebraska, Lincoln, NE (United States). Dept. of Chemical & Biomolecular Engineering

Protein tyrosine O-sulfation (PTS) plays a crucial role in extracellular biomolecular interactions that dictate various cellular processes. It also involves in the development of many human diseases. Regardless of recent progress, our current understanding of PTS is still in its infancy. To promote and facilitate relevant studies, a generally applicable method is needed to enable efficient expression of sulfoproteins with defined sulfation sites in live mammalian cells. Here we report the engineering, in vitro biochemical characterization, structural study, and in vivo functional verification of a tyrosyl-tRNA synthetase mutant for the genetic encoding of sulfotyrosine in mammalian cells. We further apply this chemical biology tool to cell-based studies on the role of a sulfation site in the activation of chemokine receptor CXCR4 by its ligand. Our work will not only facilitate cellular studies of PTS, but also paves the way for economical production of sulfated proteins as therapeutic agents in mammalian systems.

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Research Organization:: Stanford Univ., CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities Division

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1817116

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 11; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Additional file 2 of Enhanced tyrosine sulfation is associated with chronic kidney disease-related atherosclerosis Dai, Daopeng; Zhu, Zhengbin; Han, Hui figshare https://doi.org/10.6084/m9.figshare.23651446	image	January 2023
Additional file 3 of Enhanced tyrosine sulfation is associated with chronic kidney disease-related atherosclerosis Dai, Daopeng; Zhu, Zhengbin; Han, Hui figshare https://doi.org/10.6084/m9.figshare.23651449.v1	image	January 2023
Additional file 4 of Enhanced tyrosine sulfation is associated with chronic kidney disease-related atherosclerosis Dai, Daopeng; Zhu, Zhengbin; Han, Hui figshare https://doi.org/10.6084/m9.figshare.23651458	image	January 2023
Additional file 5 of Enhanced tyrosine sulfation is associated with chronic kidney disease-related atherosclerosis Dai, Daopeng; Zhu, Zhengbin; Han, Hui figshare https://doi.org/10.6084/m9.figshare.23651461	image	January 2023
Additional file 6 of Enhanced tyrosine sulfation is associated with chronic kidney disease-related atherosclerosis Dai, Daopeng; Zhu, Zhengbin; Han, Hui figshare https://doi.org/10.6084/m9.figshare.23651464.v1	image	January 2023
Additional file 7 of Enhanced tyrosine sulfation is associated with chronic kidney disease-related atherosclerosis Dai, Daopeng; Zhu, Zhengbin; Han, Hui figshare https://doi.org/10.6084/m9.figshare.23651470.v1	image	January 2023
Additional file 8 of Enhanced tyrosine sulfation is associated with chronic kidney disease-related atherosclerosis Dai, Daopeng; Zhu, Zhengbin; Han, Hui figshare https://doi.org/10.6084/m9.figshare.23651473.v1	image	January 2023
Additional file 11 of Enhanced tyrosine sulfation is associated with chronic kidney disease-related atherosclerosis Dai, Daopeng; Zhu, Zhengbin; Han, Hui figshare https://doi.org/10.6084/m9.figshare.23651482	image	January 2023
Additional file 13 of Enhanced tyrosine sulfation is associated with chronic kidney disease-related atherosclerosis Dai, Daopeng; Zhu, Zhengbin; Han, Hui figshare https://doi.org/10.6084/m9.figshare.23651488.v1	image	January 2023

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