Emergence of nosocomial associated opportunistic pathogens in the gut microbiome after antibiotic treatment
Abstract
Introduction: According to the Centers for Disease Control’s 2015 Hospital Acquired Infection Hospital Prevalence Survey, 1 in 31 hospital patients was infected with at least one nosocomial pathogen while being treated for unrelated issues. Many studies associate antibiotic administration with nosocomial infection occurrence. However, to our knowledge, there is little to no direct evidence of antibiotic administration selecting for nosocomial opportunistic pathogens. Aim: This study aims to confirm gut microbiota shifts in an animal model of antibiotic treatment to determine whether antibiotic use favors pathogenic bacteria. Methodology: We utilized next-generation sequencing and in-house metagenomic assembly and taxonomic assignment pipelines on the fecal microbiota of a urinary tract infection mouse model with and without antibiotic treatment. Results: Antibiotic therapy decreased the number of detectable species of bacteria by at least 20-fold. Furthermore, the gut microbiota of antibiotic treated mice had a significant increase of opportunistic pathogens that have been implicated in nosocomial infections, like Acinetobacter calcoaceticus/baumannii complex, Chlamydia abortus, Bacteroides fragilis, and Bacteroides thetaiotaomicron. Moreover, antibiotic treatment selected for antibiotic resistant gene enriched subpopulations for many of these opportunistic pathogens. Conclusions: Oral antibiotic therapy may select for common opportunistic pathogens responsible for nosocomial infections. In this study opportunistic pathogens present aftermore »
- Authors:
-
- U.S. Food and Drug Administration (FDA), Silver Spring, MD (United States)
- Publication Date:
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1817036
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Antimicrobial Resistance & Infection Control
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 2047-2994
- Publisher:
- BioMed Central
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Antibiotic resistance; Metagenomics; Nosocomial; Microbiota; Antibiotic therapy
Citation Formats
Raplee, Isaac, Walker, Lacey, Xu, Lei, Surathu, Anil, Chockalingam, Ashok, Stewart, Sharron, Han, Xiaomei, Rouse, Rodney, and Li, Zhihua. Emergence of nosocomial associated opportunistic pathogens in the gut microbiome after antibiotic treatment. United States: N. p., 2021.
Web. doi:10.1186/s13756-021-00903-0.
Raplee, Isaac, Walker, Lacey, Xu, Lei, Surathu, Anil, Chockalingam, Ashok, Stewart, Sharron, Han, Xiaomei, Rouse, Rodney, & Li, Zhihua. Emergence of nosocomial associated opportunistic pathogens in the gut microbiome after antibiotic treatment. United States. https://doi.org/10.1186/s13756-021-00903-0
Raplee, Isaac, Walker, Lacey, Xu, Lei, Surathu, Anil, Chockalingam, Ashok, Stewart, Sharron, Han, Xiaomei, Rouse, Rodney, and Li, Zhihua. Mon .
"Emergence of nosocomial associated opportunistic pathogens in the gut microbiome after antibiotic treatment". United States. https://doi.org/10.1186/s13756-021-00903-0. https://www.osti.gov/servlets/purl/1817036.
@article{osti_1817036,
title = {Emergence of nosocomial associated opportunistic pathogens in the gut microbiome after antibiotic treatment},
author = {Raplee, Isaac and Walker, Lacey and Xu, Lei and Surathu, Anil and Chockalingam, Ashok and Stewart, Sharron and Han, Xiaomei and Rouse, Rodney and Li, Zhihua},
abstractNote = {Introduction: According to the Centers for Disease Control’s 2015 Hospital Acquired Infection Hospital Prevalence Survey, 1 in 31 hospital patients was infected with at least one nosocomial pathogen while being treated for unrelated issues. Many studies associate antibiotic administration with nosocomial infection occurrence. However, to our knowledge, there is little to no direct evidence of antibiotic administration selecting for nosocomial opportunistic pathogens. Aim: This study aims to confirm gut microbiota shifts in an animal model of antibiotic treatment to determine whether antibiotic use favors pathogenic bacteria. Methodology: We utilized next-generation sequencing and in-house metagenomic assembly and taxonomic assignment pipelines on the fecal microbiota of a urinary tract infection mouse model with and without antibiotic treatment. Results: Antibiotic therapy decreased the number of detectable species of bacteria by at least 20-fold. Furthermore, the gut microbiota of antibiotic treated mice had a significant increase of opportunistic pathogens that have been implicated in nosocomial infections, like Acinetobacter calcoaceticus/baumannii complex, Chlamydia abortus, Bacteroides fragilis, and Bacteroides thetaiotaomicron. Moreover, antibiotic treatment selected for antibiotic resistant gene enriched subpopulations for many of these opportunistic pathogens. Conclusions: Oral antibiotic therapy may select for common opportunistic pathogens responsible for nosocomial infections. In this study opportunistic pathogens present after antibiotic therapy harbored more antibiotic resistant genes than populations of opportunistic pathogens before treatment. Our results demonstrate the effects of antibiotic therapy on induced dysbiosis and expansion of opportunistic pathogen populations and antibiotic resistant subpopulations of those pathogens. Follow-up studies with larger samples sizes and potentially controlled clinical investigations should be performed to confirm our findings.},
doi = {10.1186/s13756-021-00903-0},
journal = {Antimicrobial Resistance & Infection Control},
number = 1,
volume = 10,
place = {United States},
year = {Mon Feb 15 00:00:00 EST 2021},
month = {Mon Feb 15 00:00:00 EST 2021}
}
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