Genetic changes that increase 5-hydroxymethyl furfural resistance in ethanol-producing Escherichia coli LY180

Miller, E. N.; Turner, P. C.; Jarboe, L. R.; Ingram, L. O.

doi:10.1007/s10529-010-0209-9

Title: Genetic changes that increase 5-hydroxymethyl furfural resistance in ethanol-producing Escherichia coli LY180

Journal Article · 03 February 2010 · Biotechnology Letters

DOI: https://doi.org/10.1007/s10529-010-0209-9 · OSTI ID:1816629

Miller, E. N. ^[1]; Turner, P. C. ^[2]; Jarboe, L. R. ^[2]; Ingram, L. O. ^[2]

Univ. of Florida, Gainesville, FL (United States). Dept. of Microbiology and Cell Science; OSTI
Univ. of Florida, Gainesville, FL (United States). Dept. of Microbiology and Cell Science

The ability of a biocatalyst to tolerate furan inhibitors present in hemicellulose hydrolysates is important for the production of renewable chemicals. This study shows EMFR9, a furfural-tolerant mutant of ethanologenic E. coli LY180, has also acquired tolerance to 5-hydroxymethyl furfural (5-HMF). The mechanism of action of 5-HMF and furfural appear similar. Furan tolerance results primarily from lower expression of yqhD and dkgA, two furan reductases with a low Km for NADPH. Furan tolerance was also increased by adding plasmids encoding a NADPH/ NADH transhydrogenase (pntAB). Together, these results support the hypothesis that the NADPH-dependent reduction of furans by YqhD and DkgA inhibits growth by competing with biosynthesis for this limiting cofactor.

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Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG36-08GO88142

OSTI ID:: 1816629

Journal Information:: Biotechnology Letters, Journal Name: Biotechnology Letters Journal Issue: 5 Vol. 32; ISSN 0141-5492

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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59
Furfural
Hexose
5-Hydroxymethyl furfural
NADPH
Pentose
Transhydrogenase

Title: Genetic changes that increase 5-hydroxymethyl furfural resistance in ethanol-producing Escherichia coli LY180

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