3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation

Connor, Michael R.; Cann, Anthony F.; Liao, James C.

doi:10.1007/s00253-009-2401-1

Title: 3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation

Journal Article · 13 January 2010 · Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-009-2401-1 · OSTI ID:1816421

Connor, Michael R. ^[1]; Cann, Anthony F. ^[2]; Liao, James C. ^[2]

Univ. of California, Los Angeles, CA (United States). Dept. of Chemical and Biomolecular Engineering; OSTI
Univ. of California, Los Angeles, CA (United States). Dept. of Chemical and Biomolecular Engineering

Interest in producing biofuels from renewable sources has escalated due to energy and environmental concerns. Recently, the production of higher chain alcohols from 2-keto acid pathways has shown significant progress. In this paper, we demonstrate a mutagenesis approach in developing a strain of Escherichia coli for the production of 3-methyl-1-butanol by leveraging selective pressure toward L-leucine biosynthesis and screening for increased alcohol production. Random mutagenesis and selection with 4-aza-D,L-leucine, a structural analogue to L-leucine, resulted in the development of a new strain of E. coli able to produce 4.4 g/L of 3-methyl-1-butanol. Investigation of the host’s sensitivity to 3-methyl-1-butanol directed development of a two-phase fermentation process in which titers reached 9.5 g/L of 3-methyl-1-butanol with a yield of 0.11 g/g glucose after 60 h.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1816421

Journal Information:: Applied Microbiology and Biotechnology, Journal Name: Applied Microbiology and Biotechnology Journal Issue: 4 Vol. 86; ISSN 0175-7598

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels Atsumi, Shota; Hanai, Taizo; Liao, James C. Nature, Vol. 451, Issue 7174, p. 86-89 https://doi.org/10.1038/nature06450	journal	January 2008
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Control of gluconeogenic growth by pps and pck in Escherichia coli Chao, Y. P.; Patnaik, R.; Roof, W. D. Journal of Bacteriology, Vol. 175, Issue 21 https://doi.org/10.1128/jb.175.21.6939-6944.1993	journal	November 1993

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Engineering microbes for tolerance to next-generation biofuels Dunlop, Mary J. Biotechnology for Biofuels, Vol. 4, Issue 1 https://doi.org/10.1186/1754-6834-4-32	journal	January 2011
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Metabolic engineering for the high-yield production of isoprenoid-based C5 alcohols in E. coli George, Kevin W.; Thompson, Mitchell G.; Kang, Aram Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep11128	journal	June 2015
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Engineering microbes for tolerance to next-generation biofuels Dunlop, Mary J. Biotechnology for Biofuels, Vol. 4, Issue 1 https://doi.org/10.1186/1754-6834-4-32	journal	January 2011
Metabolic engineering of Escherichia coli for high-specificity production of isoprenol and prenol as next generation of biofuels Zheng, Yanning; Liu, Qiang; Li, Lingling Biotechnology for Biofuels, Vol. 6, Article No. 57 https://doi.org/10.1186/1754-6834-6-57	journal	January 2013
Metabolic engineering of Escherichia coli for production of mixed isoprenoid alcohols and their derivatives Zada, Bakht; Wang, Chonglong; Park, Ji-Bin Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1210-0	journal	July 2018
Biosynthesis, regulation, and engineering of microbially produced branched biofuels Bai, Wenqin; Geng, Weitao; Wang, Shaojie Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1424-9	journal	April 2019
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Title: 3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation

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References (42)

Cited By (38)

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