The foxtail millet (Setaria italica) terpene synthase gene family
Abstract
Terpenoid metabolism plays vital roles in stress defense and the environmental adaptation of monocot crops. Here, we describe the identification of the terpene synthase (TPS) gene family of the panicoid food and bioenergy model crop foxtail millet (Setaria italica). The diploid S. italica genome contains 32 TPS genes, 17 of which were biochemically characterized in this study. Unlike other thus far investigated grasses, S. italica contains TPSs producing all three ent-, (+)- and syn-copalyl pyrophosphate stereoisomers that naturally occur as central building blocks in the biosynthesis of distinct monocot diterpenoids. Conversion of these intermediates by the promiscuous TPS SiTPS8 yielded different diterpenoid scaffolds. Additionally, a cytochrome P450 monooxygenase (CYP99A17), which genomically clustered with SiTPS8, catalyzes the C19 hydroxylation of SiTPS8 products to generate the corresponding diterpene alcohols. The presence of syntenic orthologs to about 19% of the S. italica TPSs in related grasses supports a common ancestry of selected pathway branches. Among the identified enzyme products, abietadien-19-ol, syn-pimara-7,15-dien-19-ol and germacrene-D-4-ol were detectable in planta, and gene expression analysis of the biosynthetic TPSs showed distinct and, albeit moderately, inducible expression patterns in response to biotic and abiotic stress. In vitro growth-inhibiting activity of abietadien-19-ol and syn-pimara-7,15-dien-19-ol against Fusarium verticillioides and Fusariummore »
- Authors:
-
- Univ. of California, Davis, CA (United States). Dept. of Plant Biology
- Univ. of California, Davis, CA (United States). West Coast Metabolomics Center
- Publication Date:
- Research Org.:
- Univ. of California, Davis, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1816403
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- The Plant Journal
- Additional Journal Information:
- Journal Volume: 103; Journal Issue: 2; Journal ID: ISSN 0960-7412
- Publisher:
- Society for Experimental Biology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; crop stress resilience; natural products; pathway discovery; plant specialized metabolism; Setaria italica; terpene synthases
Citation Formats
Karunanithi, Prema S., Berrios, David I., Wang, Sadira, Davis, John, Shen, Tong, Fiehn, Oliver, Maloof, Julin N., and Zerbe, Philipp. The foxtail millet (Setaria italica) terpene synthase gene family. United States: N. p., 2020.
Web. doi:10.1111/tpj.14771.
Karunanithi, Prema S., Berrios, David I., Wang, Sadira, Davis, John, Shen, Tong, Fiehn, Oliver, Maloof, Julin N., & Zerbe, Philipp. The foxtail millet (Setaria italica) terpene synthase gene family. United States. https://doi.org/10.1111/tpj.14771
Karunanithi, Prema S., Berrios, David I., Wang, Sadira, Davis, John, Shen, Tong, Fiehn, Oliver, Maloof, Julin N., and Zerbe, Philipp. Mon .
"The foxtail millet (Setaria italica) terpene synthase gene family". United States. https://doi.org/10.1111/tpj.14771. https://www.osti.gov/servlets/purl/1816403.
@article{osti_1816403,
title = {The foxtail millet (Setaria italica) terpene synthase gene family},
author = {Karunanithi, Prema S. and Berrios, David I. and Wang, Sadira and Davis, John and Shen, Tong and Fiehn, Oliver and Maloof, Julin N. and Zerbe, Philipp},
abstractNote = {Terpenoid metabolism plays vital roles in stress defense and the environmental adaptation of monocot crops. Here, we describe the identification of the terpene synthase (TPS) gene family of the panicoid food and bioenergy model crop foxtail millet (Setaria italica). The diploid S. italica genome contains 32 TPS genes, 17 of which were biochemically characterized in this study. Unlike other thus far investigated grasses, S. italica contains TPSs producing all three ent-, (+)- and syn-copalyl pyrophosphate stereoisomers that naturally occur as central building blocks in the biosynthesis of distinct monocot diterpenoids. Conversion of these intermediates by the promiscuous TPS SiTPS8 yielded different diterpenoid scaffolds. Additionally, a cytochrome P450 monooxygenase (CYP99A17), which genomically clustered with SiTPS8, catalyzes the C19 hydroxylation of SiTPS8 products to generate the corresponding diterpene alcohols. The presence of syntenic orthologs to about 19% of the S. italica TPSs in related grasses supports a common ancestry of selected pathway branches. Among the identified enzyme products, abietadien-19-ol, syn-pimara-7,15-dien-19-ol and germacrene-D-4-ol were detectable in planta, and gene expression analysis of the biosynthetic TPSs showed distinct and, albeit moderately, inducible expression patterns in response to biotic and abiotic stress. In vitro growth-inhibiting activity of abietadien-19-ol and syn-pimara-7,15-dien-19-ol against Fusarium verticillioides and Fusarium subglutinans may indicate pathogen defensive functions, whereas the low antifungal efficacy of tested sesquiterpenoids supports other bioactivities. Together, these findings expand the known chemical space of monocot terpenoid metabolism to enable further investigations of terpenoid-mediated stress resilience in these agriculturally important species.},
doi = {10.1111/tpj.14771},
journal = {The Plant Journal},
number = 2,
volume = 103,
place = {United States},
year = {Mon Apr 13 00:00:00 EDT 2020},
month = {Mon Apr 13 00:00:00 EDT 2020}
}
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Identification and Regulation of TPS04/GES, an Arabidopsis Geranyllinalool Synthase Catalyzing the First Step in the Formation of the Insect-Induced Volatile C16-Homoterpene TMTT
journal, April 2008
- Herde, Marco; Gärtner, Katrin; Köllner, Tobias G.
- The Plant Cell, Vol. 20, Issue 4
Engineering Cotton (+)-δ-Cadinene Synthase to an Altered Function: Germacrene D-4-ol Synthase
journal, January 2006
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