X-ray free-electron laser studies reveal correlated motion during isopenicillin N synthase catalysis

Rabe, Patrick; Kamps, Jos J. A. G.; Sutherlin, Kyle D.; Linyard, James D. S.; Aller, Pierre; Pham, Cindy C.; Makita, Hiroki; Clifton, Ian; McDonough, Michael A.; Leissing, Thomas M.; Shutin, Denis; Lang, Pauline A.; Butryn, Agata; Brem, Jürgen; Gul, Sheraz; Fuller, Franklin D.; Kim, In-Sik; Cheah, Mun Hon; Fransson, Thomas; Bhowmick, Asmit; Young, Iris D.; O’Riordan, Lee; Brewster, Aaron S.; Pettinati, Ilaria; Doyle, Margaret; Joti, Yasumasa; Owada, Shigeki; Tono, Kensuke; Batyuk, Alexander; Hunter, Mark S.; Alonso-Mori, Roberto; Bergmann, Uwe; Owen, Robin L.; Sauter, Nicholas K.; Claridge, Timothy D. W.; Robinson, Carol V.; Yachandra, Vittal K.; Yano, Junko; Kern, Jan F.; Orville, Allen M.; Schofield, Christopher J.

doi:10.1126/sciadv.abh0250

Title: X-ray free-electron laser studies reveal correlated motion during isopenicillin N synthase catalysis

Journal Article · 19 August 2021 · Science Advances

DOI: https://doi.org/10.1126/sciadv.abh0250 · OSTI ID:1814520

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^[3]; Linyard, James D. S. ^[1]; Aller, Pierre ^[4]; Pham, Cindy C. ^[3]; Makita, Hiroki ^[3];

^[1]; McDonough, Michael A. ^[1]; Leissing, Thomas M. ^[1]; Shutin, Denis ^[1];

^[1]; Butryn, Agata ^[4];

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^[3]; Fuller, Franklin D. ^[5]; Kim, In-Sik ^[3];

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^[7]; Bhowmick, Asmit ^[3] more »

Chemistry Research Laboratory, Department of Chemistry and the Ineos Oxford Institute for Antimicrobial Research, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, UK.
Chemistry Research Laboratory, Department of Chemistry and the Ineos Oxford Institute for Antimicrobial Research, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, UK., Diamond Light Source, Diamond House, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK., Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, Oxfordshire OX11 0FA, UK.
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA.
Diamond Light Source, Diamond House, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK., Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, Oxfordshire OX11 0FA, UK.
Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Department of Chemistry - Ångström, Molecular Biomimetics, Uppsala University, SE 751 20 Uppsala, Sweden.
Interdisciplinary Center for Scientific Computing, University of Heidelberg, 69120 Heidelberg, Germany.
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA., Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198, Japan., RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Department of Physics, University of Wisconsin–Madison, 1150 University Avenue, Madison, WI 53706, USA.
Diamond Light Source, Diamond House, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK.

Complementary XFEL methods reveal O ₂ reaction intermediates and global protein dynamics during Isopenicillin N synthase catalysis.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: Biotechnology and Biological Sciences Research Council (BBSRC); Cancer Research UK; Deutsche Akademie für Naturforscher Leopoldina, Germany; Engineering and Physical Sciences Research Council (EPSRC); Ineos Oxford Institute; Medical Research Foundation (MRF); National Institutes of Health (NIH); St. John’s College; USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES); Wellcome Trust

Grant/Contract Number:: AC02-05CH11231; AC02-76SF00515

OSTI ID:: 1814520

Journal Information:: Science Advances, Journal Name: Science Advances Journal Issue: 34 Vol. 7; ISSN 2375-2548

Publisher:: American Association for the Advancement of Science (AAAS)Copyright Statement

Country of Publication:: United States

Language:: English

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