Evidence for methanobactin “Theft” and novel chalkophore production in methanotrophs: impact on methanotrophic-mediated methylmercury degradation
Abstract
Abstract Aerobic methanotrophy is strongly controlled by copper, and methanotrophs are known to use different mechanisms for copper uptake. Some methanotrophs secrete a modified polypeptide—methanobactin—while others utilize a surface-bound protein (MopE) and a secreted form of it (MopE*) for copper collection. As different methanotrophs have different means of sequestering copper, competition for copper significantly impacts methanotrophic activity. Herein, we show that Methylomicrobium album BG8, Methylocystis sp. strain Rockwell, and Methylococcus capsulatus Bath, all lacking genes for methanobactin biosynthesis, are not limited for copper by multiple forms of methanobactin. Interestingly, Mm. album BG8 and Methylocystis sp. strain Rockwell were found to have genes similar to mbnT that encodes for a TonB-dependent transporter required for methanobactin uptake. Data indicate that these methanotrophs “steal” methanobactin and such “theft” enhances the ability of these strains to degrade methylmercury, a potent neurotoxin. Further, when mbnT was deleted in Mm. album BG8, methylmercury degradation in the presence of methanobactin was indistinguishable from when MB was not added. Mc. capsulatus Bath lacks anything similar to mbnT and was unable to degrade methylmercury either in the presence or absence of methanobactin. Rather, Mc. capsulatus Bath appears to rely on MopE/MopE* for copper collection. Finally, not only does Mm.more »
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); National Science Foundation (NSF)
- OSTI Identifier:
- 2280747
- Alternate Identifier(s):
- OSTI ID: 1814404
- Grant/Contract Number:
- SC00018059; AC05-00OR22725; 1724430; 1724744; SC0018059; SC0020174
- Resource Type:
- Published Article
- Journal Name:
- The ISME Journal
- Additional Journal Information:
- Journal Name: The ISME Journal Journal Volume: 16 Journal Issue: 1; Journal ID: ISSN 1751-7362
- Publisher:
- Oxford University Press
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; bacterial genetics; biogeochemistry; soil microbiology
Citation Formats
Kang-Yun, Christina S., Liang, Xujun, Dershwitz, Philip, Gu, Wenyu, Schepers, Aloys, Flatley, Andrew, Lichtmannegger, Josef, Zischka, Hans, Zhang, Lijie, Lu, Xia, Gu, Baohua, Ledesma, Joshua C., Pelger, Daly J., DiSpirito, Alan A., and Semrau, Jeremy D. Evidence for methanobactin “Theft” and novel chalkophore production in methanotrophs: impact on methanotrophic-mediated methylmercury degradation. United Kingdom: N. p., 2021.
Web. doi:10.1038/s41396-021-01062-1.
Kang-Yun, Christina S., Liang, Xujun, Dershwitz, Philip, Gu, Wenyu, Schepers, Aloys, Flatley, Andrew, Lichtmannegger, Josef, Zischka, Hans, Zhang, Lijie, Lu, Xia, Gu, Baohua, Ledesma, Joshua C., Pelger, Daly J., DiSpirito, Alan A., & Semrau, Jeremy D. Evidence for methanobactin “Theft” and novel chalkophore production in methanotrophs: impact on methanotrophic-mediated methylmercury degradation. United Kingdom. https://doi.org/10.1038/s41396-021-01062-1
Kang-Yun, Christina S., Liang, Xujun, Dershwitz, Philip, Gu, Wenyu, Schepers, Aloys, Flatley, Andrew, Lichtmannegger, Josef, Zischka, Hans, Zhang, Lijie, Lu, Xia, Gu, Baohua, Ledesma, Joshua C., Pelger, Daly J., DiSpirito, Alan A., and Semrau, Jeremy D. Wed .
"Evidence for methanobactin “Theft” and novel chalkophore production in methanotrophs: impact on methanotrophic-mediated methylmercury degradation". United Kingdom. https://doi.org/10.1038/s41396-021-01062-1.
@article{osti_2280747,
title = {Evidence for methanobactin “Theft” and novel chalkophore production in methanotrophs: impact on methanotrophic-mediated methylmercury degradation},
author = {Kang-Yun, Christina S. and Liang, Xujun and Dershwitz, Philip and Gu, Wenyu and Schepers, Aloys and Flatley, Andrew and Lichtmannegger, Josef and Zischka, Hans and Zhang, Lijie and Lu, Xia and Gu, Baohua and Ledesma, Joshua C. and Pelger, Daly J. and DiSpirito, Alan A. and Semrau, Jeremy D.},
abstractNote = {Abstract Aerobic methanotrophy is strongly controlled by copper, and methanotrophs are known to use different mechanisms for copper uptake. Some methanotrophs secrete a modified polypeptide—methanobactin—while others utilize a surface-bound protein (MopE) and a secreted form of it (MopE*) for copper collection. As different methanotrophs have different means of sequestering copper, competition for copper significantly impacts methanotrophic activity. Herein, we show that Methylomicrobium album BG8, Methylocystis sp. strain Rockwell, and Methylococcus capsulatus Bath, all lacking genes for methanobactin biosynthesis, are not limited for copper by multiple forms of methanobactin. Interestingly, Mm. album BG8 and Methylocystis sp. strain Rockwell were found to have genes similar to mbnT that encodes for a TonB-dependent transporter required for methanobactin uptake. Data indicate that these methanotrophs “steal” methanobactin and such “theft” enhances the ability of these strains to degrade methylmercury, a potent neurotoxin. Further, when mbnT was deleted in Mm. album BG8, methylmercury degradation in the presence of methanobactin was indistinguishable from when MB was not added. Mc. capsulatus Bath lacks anything similar to mbnT and was unable to degrade methylmercury either in the presence or absence of methanobactin. Rather, Mc. capsulatus Bath appears to rely on MopE/MopE* for copper collection. Finally, not only does Mm. album BG8 steal methanobactin, it synthesizes a novel chalkophore, suggesting that some methanotrophs utilize both competition and cheating strategies for copper collection. Through a better understanding of these strategies, methanotrophic communities may be more effectively manipulated to reduce methane emissions and also enhance mercury detoxification in situ.},
doi = {10.1038/s41396-021-01062-1},
journal = {The ISME Journal},
number = 1,
volume = 16,
place = {United Kingdom},
year = {Wed Jul 21 00:00:00 EDT 2021},
month = {Wed Jul 21 00:00:00 EDT 2021}
}
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