Three mutations repurpose a plant karrikin receptor to a strigolactone receptor

Arellano-Saab, Amir; Bunsick, Michael; Al Galib, Hasan; Zhao, Wenda; Schuetz, Stefan; Bradley, James Michael; Xu, Zhenhua; Adityani, Claresta; Subha, Asrinus; McKay, Hayley; de Saint Germain, Alexandre; Boyer, François-Didier; McErlean, Christopher S. P.; Toh, Shigeo; McCourt, Peter; Stogios, Peter J.; Lumba, Shelley

doi:10.1073/pnas.2103175118

Title: Three mutations repurpose a plant karrikin receptor to a strigolactone receptor

Journal Article · 22 July 2021 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.2103175118 · OSTI ID:1809912

^[1]; Bunsick, Michael ^[2];

^[2]; Zhao, Wenda ^[2]; Schuetz, Stefan ^[2];

^[2]; Xu, Zhenhua ^[2]; Adityani, Claresta ^[2]; Subha, Asrinus ^[2]; McKay, Hayley ^[2];

^[3];

^[4];

^[5];

^[6]; McCourt, Peter ^[2];

^[7];

^[2]

Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3B2, Canada,, Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON M5S 3E5, Canada,
Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3B2, Canada,
Institut Jean-Pierre Bourgin, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, AgroParisTech, Université Paris-Saclay, 78000 Versailles, France,
Institut de Chimie des Substances Naturelles, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, France,
School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia,
Department of Environmental Bioscience, School of Agriculture, Meijo University, Nagoya 468-8502, Japan
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON M5S 3E5, Canada,

Significance Parasitic plants like witchweed cause huge losses in crop yield in Africa. A key part to the success of witchweed is to start its life cycle upon sensing small molecules called strigolactones, which are exuded from roots of host plants into the soil. Witchweed sense host-derived strigolactones through receptors called HTLs. It is thought that the evolutionary origin of HTLs is a receptor called KAI2 in nonparasitic plants, which can respond to different small molecules such as karrikins. By making three changes in the protein sequence of KAI2, this hybrid receptor can now sense both strigolactones and karrikins. These results help in understanding how receptors can evolve to sense different signals and can lead to solutions for combating pesky witchweed.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1809912

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 30 Vol. 118; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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