High titer fatty alcohol production in Lipomyces starkeyi by fed-batch fermentation
Abstract
Extracellular production of long chain fatty acid-based products, such as fatty alcohols, are being investigated as intermediates amenable to separations and catalytic upgrading to hydrocarbon fuels. We previously demonstrated that the oleaginous yeast, Lipomyces starkeyi expressing the fatty acyl-CoA reductase (FAR) gene from Marinobacter aquaeolei VT8, produced up to 770 mg/L of fatty alcohols. The FAR genes expressed in L. starkeyi transformants exhibited different fatty alcohol production levels. To better understand fatty alcohol production and potentially further increase fatty alcohol titer and yields, we investigated the expression levels, copy numbers, and enzymatic activities of the expressed FAR genes. Our results showed that fatty alcohol production is dependent on the above factors for higher titers of fatty alcohols. Fatty alcohol titer was further increased in the highest producing transformant by testing different basic process configurations (batch and fed-batch) with different initial nitrogen concentrations and feed rates. In fed-batch, a maximum of 4.2 g/L fatty alcohols were produced in 5-L bioreactor. The oleaginous yeast L. starkeyi can produce high titers of fatty alcohols particularly when periodically fed glucose and nitrogen.
- Authors:
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
- OSTI Identifier:
- 1809864
- Alternate Identifier(s):
- OSTI ID: 1782697
- Report Number(s):
- NREL/JA-2800-76592
Journal ID: ISSN 2590-2628; S2590262820300113; PII: S2590262820300113
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Published Article
- Journal Name:
- Current Research in Biotechnology
- Additional Journal Information:
- Journal Name: Current Research in Biotechnology Journal Volume: 2 Journal Issue: C; Journal ID: ISSN 2590-2628
- Publisher:
- Elsevier
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; oleaginous yeast; Lipomyces starkeyi; fatty alcohols; fed-batch fermentation
Citation Formats
Wang, Wei, Knoshaug, Eric P., Wei, Hui, Van Wychen, Stefanie, Lin, Chien-Yuan, Wall, Todd Vander, Xu, Qi, Himmel, Michael E., and Zhang, Min. High titer fatty alcohol production in Lipomyces starkeyi by fed-batch fermentation. Netherlands: N. p., 2020.
Web. doi:10.1016/j.crbiot.2020.05.002.
Wang, Wei, Knoshaug, Eric P., Wei, Hui, Van Wychen, Stefanie, Lin, Chien-Yuan, Wall, Todd Vander, Xu, Qi, Himmel, Michael E., & Zhang, Min. High titer fatty alcohol production in Lipomyces starkeyi by fed-batch fermentation. Netherlands. https://doi.org/10.1016/j.crbiot.2020.05.002
Wang, Wei, Knoshaug, Eric P., Wei, Hui, Van Wychen, Stefanie, Lin, Chien-Yuan, Wall, Todd Vander, Xu, Qi, Himmel, Michael E., and Zhang, Min. Sun .
"High titer fatty alcohol production in Lipomyces starkeyi by fed-batch fermentation". Netherlands. https://doi.org/10.1016/j.crbiot.2020.05.002.
@article{osti_1809864,
title = {High titer fatty alcohol production in Lipomyces starkeyi by fed-batch fermentation},
author = {Wang, Wei and Knoshaug, Eric P. and Wei, Hui and Van Wychen, Stefanie and Lin, Chien-Yuan and Wall, Todd Vander and Xu, Qi and Himmel, Michael E. and Zhang, Min},
abstractNote = {Extracellular production of long chain fatty acid-based products, such as fatty alcohols, are being investigated as intermediates amenable to separations and catalytic upgrading to hydrocarbon fuels. We previously demonstrated that the oleaginous yeast, Lipomyces starkeyi expressing the fatty acyl-CoA reductase (FAR) gene from Marinobacter aquaeolei VT8, produced up to 770 mg/L of fatty alcohols. The FAR genes expressed in L. starkeyi transformants exhibited different fatty alcohol production levels. To better understand fatty alcohol production and potentially further increase fatty alcohol titer and yields, we investigated the expression levels, copy numbers, and enzymatic activities of the expressed FAR genes. Our results showed that fatty alcohol production is dependent on the above factors for higher titers of fatty alcohols. Fatty alcohol titer was further increased in the highest producing transformant by testing different basic process configurations (batch and fed-batch) with different initial nitrogen concentrations and feed rates. In fed-batch, a maximum of 4.2 g/L fatty alcohols were produced in 5-L bioreactor. The oleaginous yeast L. starkeyi can produce high titers of fatty alcohols particularly when periodically fed glucose and nitrogen.},
doi = {10.1016/j.crbiot.2020.05.002},
journal = {Current Research in Biotechnology},
number = C,
volume = 2,
place = {Netherlands},
year = {Sun Nov 01 00:00:00 EDT 2020},
month = {Sun Nov 01 00:00:00 EDT 2020}
}
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