Active liquid crystals powered by force-sensing DNA-motor clusters

Tayar, Alexandra M.; Hagan, Michael F.; Dogic, Zvonimir

doi:10.1073/pnas.2102873118

Title: Active liquid crystals powered by force-sensing DNA-motor clusters

Journal Article · 20 July 2021 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.2102873118 · OSTI ID:1808734

^[1];

^[2]; Dogic, Zvonimir ^[3]

Department of Physics, University of California, Santa Barbara, CA 93106,
Department of Physics, Brandeis University, Waltham, MA 02453
Department of Physics, University of California, Santa Barbara, CA 93106,, Department of Physics, Brandeis University, Waltham, MA 02453

Significance Single-molecule techniques have elucidated how isolated molecular motors generate piconewton forces with unprecedented detail. However, in diverse biological and synthetic settings, force-generating proteins collectively power nonequilibrium dynamics, including continuous large-scale rearrangements and persistent fluid flows. Characterizing motor-generated forces in these dense and dynamical environments remains a challenge. We assembled a reversible DNA-based force-sensing probe that, by an optical readout, reveals the molecular arrangements and the force loads experienced by kinesin motors. These probes provide insight into motor-generated forces that collectively power the unique dynamics of microtubule-based active nematics, a noteworthy example of an internally driven active matter system. DNA-based force probes can be extended to study forces and stresses in various synthetic systems as well as diverse cellular environments.

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Research Organization:: Univ. of California, Santa Barbara, CA (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: SC0019733

OSTI ID:: 1808734

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 30 Vol. 118; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Active liquid crystals powered by force-sensing DNA-motor clusters

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