Title: Cysteine Mutants of the Major Facilitator Superfamily-Type Transporter CcoA Provide Insight into Copper Import

Journal Article · · mBio (Online)
 [1];  [2];  [2];  [3];  [2];
  1. Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA, Université de Pau et des Pays de l’Adour, E2S UPPA, IPREM, UMR CNRS 5254, Pau, France
  2. Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
  3. Institute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, Albert Ludwigs University of Freiburg, Freiburg, Germany
+ Show Author Affiliations

Copper (Cu) is a redox-active micronutrient that is both essential and toxic. Its cellular homeostasis is critical for supporting cuproprotein maturation while avoiding excessive oxidative stress. The Cu importer CcoA is the prototype of the widespread CalT subfamily of the MFS-type transporters. Hence, understanding its molecular mechanism of function is significant. Here, we show that CcoA undergoes a thiol:disulfide oxidoreduction cycle, which is important for its Cu import activity.

Research Organization:
University of Pennsylvania, Philadelphia, PA (United States)
Sponsoring Organization:
German Research Foundation (DFG); National Institutes of Health (NIH); USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division
Grant/Contract Number:
FG02-91ER20052
OSTI ID:
1808722
Journal Information:
mBio (Online), Journal Name: mBio (Online) Journal Issue: 4 Vol. 12; ISSN 2150-7511
Publisher:
American Society for MicrobiologyCopyright Statement
Country of Publication:
United States
Language:
English

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MFS-type transporters
59 BASIC BIOLOGICAL SCIENCES
Rhodobacter capsulatus
Rhodobacter capsulatus CcoA
bacterial copper homeostasis
bacterial copper import
cbb3-type cytochrome c oxidase
copper binding residues
copper uptake
copper-binding residues