Renewable fatty acid ester production in Clostridium

Feng, Jun; Zhang, Jie; Ma, Yuechao; Feng, Yiming; Wang, Shangjun; Guo, Na; Wang, Haijiao; Wang, Pixiang; Jiménez-Bonilla, Pablo; Gu, Yanyan; Zhou, Junping; Zhang, Zhong-Tian; Cao, Mingfeng; Jiang, Di; Wang, Shuning; Liu, Xian-Wei; Shao, Zengyi; Borovok, Ilya; Huang, Haibo; Wang, Yi

doi:10.1038/s41467-021-24038-3

Title: Renewable fatty acid ester production in Clostridium

Journal Article · 15 July 2021 · Nature Communications

DOI: https://doi.org/10.1038/s41467-021-24038-3 · OSTI ID:1808329

Feng, Jun; Zhang, Jie; Ma, Yuechao; Feng, Yiming; Wang, Shangjun; Guo, Na; Wang, Haijiao; Wang, Pixiang; Jiménez-Bonilla, Pablo; Gu, Yanyan; Zhou, Junping; Zhang, Zhong-Tian;

; Jiang, Di; Wang, Shuning; Liu, Xian-Wei;

;

; Huang, Haibo;

Abstract Bioproduction of renewable chemicals is considered as an urgent solution for fossil energy crisis. However, despite tremendous efforts, it is still challenging to generate microbial strains that can produce target biochemical to high levels. Here, we report an example of biosynthesis of high-value and easy-recoverable derivatives built upon natural microbial pathways, leading to improvement in bioproduction efficiency. By leveraging pathways in solventogenic clostridia for co-producing acyl-CoAs, acids and alcohols as precursors, through rational screening for host strains and enzymes, systematic metabolic engineering-including elimination of putative prophages, we develop strains that can produce 20.3 g/L butyl acetate and 1.6 g/L butyl butyrate. Techno-economic analysis results suggest the economic competitiveness of our developed bioprocess. Our principles of selecting the most appropriate host for specific bioproduction and engineering microbial chassis to produce high-value and easy-separable end products may be applicable to other bioprocesses.

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Research Organization:: Auburn Univ., AL (United States)

Sponsoring Organization:: USDOE; USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: EE0008483

OSTI ID:: 1808329

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 12; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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