Stepping on the Gas to a Circular Economy: Accelerating Development of Carbon-Negative Chemical Production from Gas Fermentation
Abstract
Owing to rising levels of greenhouse gases in our atmosphere and oceans, climate change poses significant environmental, economic, and social challenges globally. Technologies that enable carbon capture and conversion of greenhouse gases into useful products will help mitigate climate change by enabling a new circular carbon economy. Gas fermentation usingcarbon-fixing microorganisms offers an economically viable and scalable solution with unique feedstock and product flexibility that has been commercialized recently. We review the state of the art of gas fermentation and discuss opportunities to accelerate future development and rollout. We discuss the current commercial process for conversion of waste gases to ethanol, including the underlying biology, challenges in process scale-up, and progress on genetic tool development and metabolic engineering to expand the product spectrum. We emphasize key enabling technologies to accelerate strain development for acetogens and other nonmodel organisms.
- Authors:
-
- LanzaTech Inc., Skokie, IL (United States)
- Northwestern Univ., Evanston, IL (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Northwestern Univ., Evanston, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1807218
- Alternate Identifier(s):
- OSTI ID: 2298995; OSTI ID: 2301599
- Grant/Contract Number:
- AC05-00OR22725; SC0018249; EE0008343; EE0008354
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Annual Review of Chemical and Biomolecular Engineering
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 1; Journal ID: ISSN 1947-5438
- Publisher:
- Annual Reviews
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; gas fermentation; syngas; acetogen; clostridium; cell-free; CRISPR
Citation Formats
Fackler, Nick, Heijstra, Björn D., Rasor, Blake J., Brown, Hunter, Martin, Jacob, Ni, Zhuofu, Shebek, Kevin M., Rosin, Rick R., Simpson, Séan D., Tyo, Keith E., Giannone, Richard J., Hettich, Robert L., Tschaplinski, Timothy J., Leang, Ching, Brown, Steven D., Jewett, Michael C., and Köpke, Michael. Stepping on the Gas to a Circular Economy: Accelerating Development of Carbon-Negative Chemical Production from Gas Fermentation. United States: N. p., 2021.
Web. doi:10.1146/annurev-chembioeng-120120-021122.
Fackler, Nick, Heijstra, Björn D., Rasor, Blake J., Brown, Hunter, Martin, Jacob, Ni, Zhuofu, Shebek, Kevin M., Rosin, Rick R., Simpson, Séan D., Tyo, Keith E., Giannone, Richard J., Hettich, Robert L., Tschaplinski, Timothy J., Leang, Ching, Brown, Steven D., Jewett, Michael C., & Köpke, Michael. Stepping on the Gas to a Circular Economy: Accelerating Development of Carbon-Negative Chemical Production from Gas Fermentation. United States. https://doi.org/10.1146/annurev-chembioeng-120120-021122
Fackler, Nick, Heijstra, Björn D., Rasor, Blake J., Brown, Hunter, Martin, Jacob, Ni, Zhuofu, Shebek, Kevin M., Rosin, Rick R., Simpson, Séan D., Tyo, Keith E., Giannone, Richard J., Hettich, Robert L., Tschaplinski, Timothy J., Leang, Ching, Brown, Steven D., Jewett, Michael C., and Köpke, Michael. Mon .
"Stepping on the Gas to a Circular Economy: Accelerating Development of Carbon-Negative Chemical Production from Gas Fermentation". United States. https://doi.org/10.1146/annurev-chembioeng-120120-021122. https://www.osti.gov/servlets/purl/1807218.
@article{osti_1807218,
title = {Stepping on the Gas to a Circular Economy: Accelerating Development of Carbon-Negative Chemical Production from Gas Fermentation},
author = {Fackler, Nick and Heijstra, Björn D. and Rasor, Blake J. and Brown, Hunter and Martin, Jacob and Ni, Zhuofu and Shebek, Kevin M. and Rosin, Rick R. and Simpson, Séan D. and Tyo, Keith E. and Giannone, Richard J. and Hettich, Robert L. and Tschaplinski, Timothy J. and Leang, Ching and Brown, Steven D. and Jewett, Michael C. and Köpke, Michael},
abstractNote = {Owing to rising levels of greenhouse gases in our atmosphere and oceans, climate change poses significant environmental, economic, and social challenges globally. Technologies that enable carbon capture and conversion of greenhouse gases into useful products will help mitigate climate change by enabling a new circular carbon economy. Gas fermentation usingcarbon-fixing microorganisms offers an economically viable and scalable solution with unique feedstock and product flexibility that has been commercialized recently. We review the state of the art of gas fermentation and discuss opportunities to accelerate future development and rollout. We discuss the current commercial process for conversion of waste gases to ethanol, including the underlying biology, challenges in process scale-up, and progress on genetic tool development and metabolic engineering to expand the product spectrum. We emphasize key enabling technologies to accelerate strain development for acetogens and other nonmodel organisms.},
doi = {10.1146/annurev-chembioeng-120120-021122},
journal = {Annual Review of Chemical and Biomolecular Engineering},
number = 1,
volume = 12,
place = {United States},
year = {Mon Apr 19 00:00:00 EDT 2021},
month = {Mon Apr 19 00:00:00 EDT 2021}
}
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Works referenced in this record:
Climate damages and adaptation potential across diverse sectors of the United States
journal, April 2019
- Martinich, Jeremy; Crimmins, Allison
- Nature Climate Change, Vol. 9, Issue 5
Temporary reduction in daily global CO2 emissions during the COVID-19 forced confinement
journal, May 2020
- Le Quéré, Corinne; Jackson, Robert B.; Jones, Matthew W.
- Nature Climate Change, Vol. 10, Issue 7
Rapid cost decrease of renewables and storage accelerates the decarbonization of China’s power system
journal, May 2020
- He, Gang; Lin, Jiang; Sifuentes, Froylan
- Nature Communications, Vol. 11, Issue 1
Environmental co-benefits and adverse side-effects of alternative power sector decarbonization strategies
journal, November 2019
- Luderer, Gunnar; Pehl, Michaja; Arvesen, Anders
- Nature Communications, Vol. 10, Issue 1
Grand Research Challenges for Sustainable Industrial Biotechnology
journal, October 2019
- Straathof, Adrie J. J.; Wahl, S. Aljoscha; Benjamin, Kirsten R.
- Trends in Biotechnology, Vol. 37, Issue 10
What is still Limiting the Deployment of Cellulosic Ethanol? Analysis of the Current Status of the Sector
journal, October 2019
- Padella, Monica; O’Connell, Adrian; Prussi, Matteo
- Applied Sciences, Vol. 9, Issue 21
Making quantitative sense of electromicrobial production
journal, May 2019
- Claassens, Nico J.; Cotton, Charles A. R.; Kopljar, Dennis
- Nature Catalysis, Vol. 2, Issue 5
Stoichiometric and energetic analyses of non-photosynthetic CO2-fixation pathways to support synthetic biology strategies for production of fuels and chemicals
journal, November 2012
- Fast, Alan G.; Papoutsakis, Eleftherios T.
- Current Opinion in Chemical Engineering, Vol. 1, Issue 4
A survey of carbon fixation pathways through a quantitative lens
journal, December 2011
- Bar-Even, Arren; Noor, Elad; Milo, Ron
- Journal of Experimental Botany, Vol. 63, Issue 6, p. 2325-2342
Perspectives for the biotechnological production of biofuels from CO2 and H2 using Ralstonia eutropha and other ‘Knallgas’ bacteria
journal, January 2019
- Brigham, Christopher
- Applied Microbiology and Biotechnology, Vol. 103, Issue 5
C1-carbon sources for chemical and fuel production by microbial gas fermentation
journal, December 2015
- Dürre, Peter; Eikmanns, Bernhard J.
- Current Opinion in Biotechnology, Vol. 35
Microbial CO Conversions with Applications in Synthesis Gas Purification and Bio-Desulfurization
journal, January 2006
- Sipma, Jan; Henstra, Anne M.; Parshina, Sofiya N.
- Critical Reviews in Biotechnology, Vol. 26, Issue 1
A methanotroph-based biorefinery: Potential scenarios for generating multiple products from a single fermentation
journal, September 2016
- Strong, P. J.; Kalyuzhnaya, M.; Silverman, J.
- Bioresource Technology, Vol. 215
Metabolic engineering in methanotrophic bacteria
journal, May 2015
- Kalyuzhnaya, Marina G.; Puri, Aaron W.; Lidstrom, Mary E.
- Metabolic Engineering, Vol. 29
Metabolic versatility in methanogens
journal, October 2014
- Costa, Kyle C.; Leigh, John A.
- Current Opinion in Biotechnology, Vol. 29
Acetogenic Prokaryotes
book, January 2006
- Drake, Harold L.; Küsel, Kirsten; Matthies, Carola
- The Prokaryotes
The rocky roots of the acetyl-CoA pathway
journal, July 2004
- Russell, Michael J.; Martin, William
- Trends in Biochemical Sciences, Vol. 29, Issue 7
Older Than Genes: The Acetyl CoA Pathway and Origins
journal, June 2020
- Martin, William F.
- Frontiers in Microbiology, Vol. 11
A hydrogen-dependent geochemical analogue of primordial carbon and energy metabolism
journal, March 2020
- Preiner, Martina; Igarashi, Kensuke; Muchowska, Kamila B.
- Nature Ecology & Evolution, Vol. 4, Issue 4
Metal centers in the anaerobic microbial metabolism of CO and CO2
journal, January 2011
- Bender, Güneş; Pierce, Elizabeth; Hill, Jeffrey A.
- Metallomics, Vol. 3, Issue 8
Acetogenesis and the Wood–Ljungdahl pathway of CO2 fixation
journal, December 2008
- Ragsdale, Stephen W.; Pierce, Elizabeth
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1784, Issue 12, p. 1873-1898
Life with CO or CO2 and H2 as a source of carbon and energy.
journal, February 1991
- Wood, H. G.
- The FASEB Journal, Vol. 5, Issue 2
The Complete Genome Sequence of Clostridium aceticum: a Missing Link between Rnf- and Cytochrome-Containing Autotrophic Acetogens
journal, September 2015
- Poehlein, Anja; Cebulla, Martin; Ilg, Marcus M.
- mBio, Vol. 6, Issue 5
Analysis of the Core Genome and Pan-Genome of Autotrophic Acetogenic Bacteria
journal, September 2016
- Shin, Jongoh; Song, Yoseb; Jeong, Yujin
- Frontiers in Microbiology, Vol. 7
Methanol and methyl group conversion in acetogenic bacteria: biochemistry, physiology and application
journal, September 2020
- Kremp, Florian; Müller, Volker
- FEMS Microbiology Reviews, Vol. 45, Issue 2
Functional cooperation of the glycine synthase-reductase and Wood–Ljungdahl pathways for autotrophic growth of Clostridium drakei
journal, March 2020
- Song, Yoseb; Lee, Jin Soo; Shin, Jongoh
- Proceedings of the National Academy of Sciences, Vol. 117, Issue 13
Acetogenic mixotrophy: novel options for yield improvement in biofuels and biochemicals production
journal, June 2015
- Fast, Alan G.; Schmidt, Ellinor D.; Jones, Shawn W.
- Current Opinion in Biotechnology, Vol. 33
Methanogenesis and the Wood–Ljungdahl Pathway: An Ancient, Versatile, and Fragile Association
journal, May 2016
- Borrel, Guillaume; Adam, Panagiotis S.; Gribaldo, Simonetta
- Genome Biology and Evolution, Vol. 8, Issue 6
Autotrophy at the thermodynamic limit of life: a model for energy conservation in acetogenic bacteria
journal, November 2014
- Schuchmann, Kai; Müller, Volker
- Nature Reviews Microbiology, Vol. 12, Issue 12
Discovery of a Ferredoxin:NAD+-Oxidoreductase (Rnf) in Acetobacterium woodii: A Novel Potential Coupling Site in Acetogens
journal, March 2008
- Müller, Volker; Imkamp, Frank; Biegel, Eva
- Annals of the New York Academy of Sciences, Vol. 1125, Issue 1
Coupled Ferredoxin and Crotonyl Coenzyme A (CoA) Reduction with NADH Catalyzed by the Butyryl-CoA Dehydrogenase/Etf Complex from Clostridium kluyveri
journal, November 2007
- Li, F.; Hinderberger, J.; Seedorf, H.
- Journal of Bacteriology, Vol. 190, Issue 3
Flavin-Based Electron Bifurcation, Ferredoxin, Flavodoxin, and Anaerobic Respiration With Protons (Ech) or NAD+ (Rnf) as Electron Acceptors: A Historical Review
journal, March 2018
- Buckel, Wolfgang; Thauer, Rudolf K.
- Frontiers in Microbiology, Vol. 9
Electron bifurcation
journal, April 2016
- Peters, John W.; Miller, Anne-Frances; Jones, Anne K.
- Current Opinion in Chemical Biology, Vol. 31
NADP-Specific Electron-Bifurcating [FeFe]-Hydrogenase in a Functional Complex with Formate Dehydrogenase in Clostridium autoethanogenum Grown on CO
journal, July 2013
- Wang, S.; Huang, H.; Kahnt, J.
- Journal of Bacteriology, Vol. 195, Issue 19
Electron Bifurcation Involved in the Energy Metabolism of the Acetogenic Bacterium Moorella thermoacetica Growing on Glucose or H2 plus CO2
journal, May 2012
- Huang, H.; Wang, S.; Moll, J.
- Journal of Bacteriology, Vol. 194, Issue 14
A Bacterial Electron-bifurcating Hydrogenase
journal, July 2012
- Schuchmann, Kai; Müller, Volker
- Journal of Biological Chemistry, Vol. 287, Issue 37
The Sporomusa type Nfn is a novel type of electron-bifurcating transhydrogenase that links the redox pools in acetogenic bacteria
journal, September 2020
- Kremp, Florian; Roth, Jennifer; Müller, Volker
- Scientific Reports, Vol. 10, Issue 1
Redox controls metabolic robustness in the gas-fermenting acetogen Clostridium autoethanogenum
journal, May 2020
- Mahamkali, Vishnuvardhan; Valgepea, Kaspar; de Souza Pinto Lemgruber, Renato
- Proceedings of the National Academy of Sciences, Vol. 117, Issue 23
Energy Conservation Associated with Ethanol Formation from H 2 and CO 2 in Clostridium autoethanogenum Involving Electron Bifurcation
journal, July 2015
- Mock, Johanna; Zheng, Yanning; Mueller, Alexander P.
- Journal of Bacteriology, Vol. 197, Issue 18
The role of tungstate and/or molybdate in the formation of aldehyde oxidoreductase in Clostridium thermoaceticum and other acetogens; immunological distances of such enzymes
journal, July 1992
- White, Hiltrud; Simon, Helmut
- Archives of Microbiology, Vol. 158, Issue 2
The Wood–Ljungdahl pathway as a key component of metabolic versatility in candidate phylum Bipolaricaulota (Acetothermia, OP1)
journal, April 2019
- Youssef, Noha H.; Farag, Ibrahim F.; Rudy, Sydney
- Environmental Microbiology Reports, Vol. 11, Issue 4
Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications
journal, June 2012
- Tracy, Bryan P.; Jones, Shawn W.; Fast, Alan G.
- Current Opinion in Biotechnology, Vol. 23, Issue 3
Low carbon fuels and commodity chemicals from waste gases – systematic approach to understand energy metabolism in a model acetogen
journal, January 2016
- Marcellin, Esteban; Behrendorff, James B.; Nagaraju, Shilpa
- Green Chemistry, Vol. 18, Issue 10
Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia
journal, January 2014
- Brown, Steven D.; Nagaraju, Shilpa; Utturkar, Sagar
- Biotechnology for Biofuels, Vol. 7, Issue 1
Clostridium autoethanogenum, sp. nov., an anaerobic bacterium that produces ethanol from carbon monoxide
journal, April 1994
- Abrini, Jamal; Naveau, Henry; Nyns, Edmond-Jacques
- Archives of Microbiology, Vol. 161, Issue 4
Clostridium ljungdahlii sp. nov., an Acetogenic Species in Clostridial rRNA Homology Group I
journal, April 1993
- Tanner, R. S.; Miller, L. M.; Yang, D.
- International Journal of Systematic Bacteriology, Vol. 43, Issue 2
Clostridium ljungdahlii represents a microbial production platform based on syngas
journal, July 2010
- Kopke, M.; Held, C.; Hujer, S.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 29, p. 13087-13092
Engineering Clostridium ljungdahlii as the gas-fermenting cell factory for the production of biofuels and biochemicals
journal, December 2020
- Zhang, Lu; Zhao, Ran; Jia, Dechen
- Current Opinion in Chemical Biology, Vol. 59
Industrial Acetogenic Biocatalysts: A Comparative Metabolic and Genomic Analysis
journal, July 2016
- Bengelsdorf, Frank R.; Poehlein, Anja; Linder, Sonja
- Frontiers in Microbiology, Vol. 7
Traits of selected Clostridium strains for syngas fermentation to ethanol : Syngas Fermentation Strain Comparison
journal, September 2015
- Martin, Michael E.; Richter, Hanno; Saha, Surya
- Biotechnology and Bioengineering, Vol. 113, Issue 3
Clostridium carboxidivorans sp. nov., a solvent-producing clostridium isolated from an agricultural settling lagoon, and reclassification of the acetogen Clostridium scatologenes strain SL1 as Clostridium drakei sp. nov.
journal, September 2005
- Liou, J. S. -C.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 55, Issue 5
Effects of moderately elevated pressure on gas fermentation processes
journal, December 2019
- Van Hecke, Wouter; Bockrath, Richard; De Wever, Heleen
- Bioresource Technology, Vol. 293
Side-by-Side Comparison of Clean and Biomass-Derived, Impurity-Containing Syngas as Substrate for Acetogenic Fermentation with Clostridium ljungdahlii
journal, August 2020
- Infantes, Alba; Kugel, Michaela; Raffelt, Klaus
- Fermentation, Vol. 6, Issue 3
Pollution to products: recycling of ‘above ground’ carbon by gas fermentation
journal, October 2020
- Köpke, Michael; Simpson, Séan D.
- Current Opinion in Biotechnology, Vol. 65
The effects of syngas impurities on syngas fermentation to liquid fuels
journal, July 2011
- Xu, Deshun; Tree, Douglas R.; Lewis, Randy S.
- Biomass and Bioenergy, Vol. 35, Issue 7
Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks
journal, May 2016
- Liew, FungMin; Martin, Michael E.; Tappel, Ryan C.
- Frontiers in Microbiology, Vol. 7
Bioreactor design for synthesis gas fermentations
journal, May 1991
- Klasson, K. T.; Ackerson, M. D.; Clausen, E. C.
- Fuel, Vol. 70, Issue 5
Bioconversion of synthesis gas into liquid or gaseous fuels
journal, August 1992
- Klasson, K. Thomas; Ackerson, Michael D.; Clausen, Edgar C.
- Enzyme and Microbial Technology, Vol. 14, Issue 8, p. 602-608
Gas fermentation of C1 feedstocks: commercialization status and future prospects: Commercialization Status of Gas fermentation of C1 feedstocks
journal, July 2018
- Teixeira, Leonardo V.; Moutinho, Liza F.; Romão-Dumaresq, Aline S.
- Biofuels, Bioproducts and Biorefining, Vol. 12, Issue 6
Syngas Fermentation to Alcohols: Reactor Technology and Application Perspective
journal, January 2020
- Stoll, I. Katharina; Boukis, Nikolaos; Sauer, Jörg
- Chemie Ingenieur Technik, Vol. 92, Issue 1-2
Alternative thermochemical routes for aviation biofuels via alcohols synthesis: Process modeling, techno-economic assessment and comparison
journal, January 2015
- Atsonios, Konstantinos; Kougioumtzis, Michael-Alexander; D. Panopoulos, Kyriakos
- Applied Energy, Vol. 138
Combining microbial production with chemical upgrading
journal, April 2016
- Goulas, Konstantinos A.; Toste, F. Dean
- Current Opinion in Biotechnology, Vol. 38
Coupling chemical and biological catalysis: a flexible paradigm for producing biobased chemicals
journal, April 2016
- Schwartz, Thomas J.; Shanks, Brent H.; Dumesic, James A.
- Current Opinion in Biotechnology, Vol. 38
Ethanol as a Renewable Building Block for Fuels and Chemicals
journal, February 2020
- Dagle, Robert A.; Winkelman, Austin D.; Ramasamy, Karthikeyan K.
- Industrial & Engineering Chemistry Research, Vol. 59, Issue 11
Technical photosynthesis involving CO2 electrolysis and fermentation
journal, January 2018
- Haas, Thomas; Krause, Ralf; Weber, Rainer
- Nature Catalysis, Vol. 1, Issue 1
Partial replacement of fishmeal with Clostridium autoethanogenum single‐cell protein in the diet for juvenile black sea bream ( Acanthopagrus schlegelii )
journal, December 2019
- Chen, Ying; Sagada, Gladstone; Xu, Bingying
- Aquaculture Research, Vol. 51, Issue 3
Diet supplemented with a novel Clostridium autoethanogenum protein have a positive effect on the growth performance, antioxidant status and immunity in juvenile Jian carp (Cyprinus carpio var. Jian)
journal, March 2021
- Li, Miaoyu; Liang, Hualiang; Xie, Jun
- Aquaculture Reports, Vol. 19
Metabolic engineering of Clostridium autoethanogenum for selective alcohol production
journal, March 2017
- Liew, Fungmin; Henstra, Anne M.; Kӧpke, Michael
- Metabolic Engineering, Vol. 40
Rational development of transformation in Clostridium thermocellum ATCC 27405 via complete methylome analysis and evasion of native restriction–modification systems
journal, July 2019
- Riley, Lauren A.; Ji, Lexiang; Schmitz, Robert J.
- Journal of Industrial Microbiology & Biotechnology, Vol. 46, Issue 9-10
A novel conjugal donor strain for improved DNA transfer into Clostridium spp.
journal, October 2019
- Woods, Craig; Humphreys, Christopher M.; Rodrigues, Raquel Mesquita
- Anaerobe, Vol. 59
A Genetic System for Clostridium ljungdahlii: a Chassis for Autotrophic Production of Biocommodities and a Model Homoacetogen
journal, November 2012
- Leang, Ching; Ueki, Toshiyuki; Nevin, Kelly P.
- Applied and Environmental Microbiology, Vol. 79, Issue 4
Expanding the molecular toolkit for the homoacetogen Clostridium ljungdahlii
journal, August 2016
- Molitor, Bastian; Kirchner, Kristina; Henrich, Alexander W.
- Scientific Reports, Vol. 6, Issue 1
Thermophilic ethanol fermentation from lignocellulose hydrolysate by genetically engineered Moorella thermoacetica
journal, December 2017
- Rahayu, Farida; Kawai, Yuto; Iwasaki, Yuki
- Bioresource Technology, Vol. 245
A Strongly Fluorescing Anaerobic Reporter and Protein-Tagging System for Clostridium Organisms Based on the Fluorescence-Activating and Absorption-Shifting Tag Protein (FAST)
journal, May 2019
- Streett, Hannah E.; Kalis, Katie M.; Papoutsakis, Eleftherios T.
- Applied and Environmental Microbiology, Vol. 85, Issue 14
Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag and SNAP-tag Proteins
journal, October 2020
- Charubin, Kamil; Streett, Hannah; Papoutsakis, Eleftherios Terry
- Applied and Environmental Microbiology, Vol. 86, Issue 20
The ClosTron: Mutagenesis in Clostridium refined and streamlined
journal, January 2010
- Heap, John T.; Kuehne, Sarah A.; Ehsaan, Muhammad
- Journal of Microbiological Methods, Vol. 80, Issue 1
The ClosTron: A universal gene knock-out system for the genus Clostridium
journal, September 2007
- Heap, John T.; Pennington, Oliver J.; Cartman, Stephen T.
- Journal of Microbiological Methods, Vol. 70, Issue 3
Group II Intron-Anchored Gene Deletion in Clostridium
journal, January 2011
- Jia, Kaizhi; Zhu, Yan; Zhang, Yanping
- PLoS ONE, Vol. 6, Issue 1
Inactivation of an aldehyde/alcohol dehydrogenase gene fromClostridium acetobutylicum ATCC 824
journal, March 1996
- Green, Edward M.; Bennett, George N.
- Applied Biochemistry and Biotechnology, Vol. 57-58, Issue 1
Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824
journal, August 1996
- Green, E. M.; Boynton, Z. L.; Harris, L. M.
- Microbiology, Vol. 142, Issue 8
Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations
journal, January 2009
- Sillers, Ryan; Al-Hinai, Mohab Ali; Papoutsakis, Eleftherios T.
- Biotechnology and Bioengineering, Vol. 102, Issue 1, p. 38-49
Effects of different replicons in conjugative plasmids on transformation efficiency, plasmid stability, gene expression and n-butanol biosynthesis in Clostridium tyrobutyricum
journal, December 2011
- Yu, Mingrui; Du, Yinming; Jiang, Wenyan
- Applied Microbiology and Biotechnology, Vol. 93, Issue 2
Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker
journal, January 2012
- Heap, John T.; Ehsaan, Muhammad; Cooksley, Clare M.
- Nucleic Acids Research, Vol. 40, Issue 8
Group I introns as mobile genetic elements: Facts and mechanistic speculations — a review
journal, October 1989
- Dujon, Bernard
- Gene, Vol. 82, Issue 1
I-SceI-mediated scarless gene modification via allelic exchange in Clostridium
journal, January 2015
- Zhang, Ning; Shao, Lijun; Jiang, Yu
- Journal of Microbiological Methods, Vol. 108
Converting Carbon Dioxide to Butyrate with an Engineered Strain of Clostridium ljungdahlii
journal, October 2014
- Ueki, Toshiyuki; Nevin, Kelly P.; Woodard, Trevor L.
- mBio, Vol. 5, Issue 5
Phage serine integrase-mediated genome engineering for efficient expression of chemical biosynthetic pathway in gas-fermenting Clostridium ljungdahlii
journal, March 2019
- Huang, He; Chai, Changsheng; Yang, Sheng
- Metabolic Engineering, Vol. 52
CRAGE enables rapid activation of biosynthetic gene clusters in undomesticated bacteria
journal, October 2019
- Wang, Gaoyan; Zhao, Zhiying; Ke, Jing
- Nature Microbiology, Vol. 4, Issue 12
Multi-chassis engineering for heterologous production of microbial natural products
journal, April 2020
- Ke, Jing; Yoshikuni, Yasuo
- Current Opinion in Biotechnology, Vol. 62
Development of a high efficiency integration system and promoter library for rapid modification of Pseudomonas putida KT2440
journal, December 2017
- Elmore, Joshua R.; Furches, Anna; Wolff, Gara N.
- Metabolic Engineering Communications, Vol. 5
Development of a metabolic pathway transfer and genomic integration system for the syngas-fermenting bacterium Clostridium ljungdahlii
journal, May 2019
- Philipps, Gabriele; de Vries, Sebastian; Jennewein, Stefan
- Biotechnology for Biofuels, Vol. 12, Issue 1
A Universal Mariner Transposon System for Forward Genetic Studies in the Genus Clostridium
journal, April 2015
- Zhang, Ying; Grosse-Honebrink, Alexander; Minton, Nigel P.
- PLOS ONE, Vol. 10, Issue 4
A
m
ariner
-Based Transposon System for
In Vivo
Random Mutagenesis of
Clostridium difficile
journal, February 2010
- Cartman, Stephen T.; Minton, Nigel P.
- Applied and Environmental Microbiology, Vol. 76, Issue 4
In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design
journal, June 2020
- Karim, Ashty S.; Dudley, Quentin M.; Juminaga, Alex
- Nature Chemical Biology, Vol. 16, Issue 8
Biocatalytic production of isoprene by fermentation of metabolically engineered Clostridium ljungdahlii on syngas
journal, August 2020
- Philipps, G.; Janke, C.; Jennewein, S.
- Chemie Ingenieur Technik, Vol. 92, Issue 9
Synthesis of Heterologous Mevalonic Acid Pathway Enzymes in Clostridium ljungdahlii for the Conversion of Fructose and of Syngas to Mevalonate and Isoprene
journal, January 2018
- Diner, Bruce A.; Fan, Janine; Scotcher, Miles C.
- Applied and Environmental Microbiology, Vol. 84, Issue 1
Effects of hydrogen partial pressure on autotrophic growth and product formation of Acetobacterium woodii
journal, April 2016
- Kantzow, Christina; Weuster-Botz, Dirk
- Bioprocess and Biosystems Engineering, Vol. 39, Issue 8
Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum
journal, April 2019
- Annan, Florence J.; Al-Sinawi, Bakir; Humphreys, Christopher M.
- Applied Microbiology and Biotechnology, Vol. 103, Issue 11
CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea
journal, February 2010
- Marraffini, Luciano A.; Sontheimer, Erik J.
- Nature Reviews Genetics, Vol. 11, Issue 3
A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
journal, June 2012
- Jinek, M.; Chylinski, K.; Fonfara, I.
- Science, Vol. 337, Issue 6096, p. 816-821
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
journal, February 2013
- Qi, Lei S.; Larson, Matthew H.; Gilbert, Luke A.
- Cell, Vol. 152, Issue 5, p. 1173-1183
Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system
journal, June 2013
- Bikard, David; Jiang, Wenyan; Samai, Poulami
- Nucleic Acids Research, Vol. 41, Issue 15
Making Ends Meet: Repairing Breaks in Bacterial DNA by Non-Homologous End-Joining
journal, February 2006
- Bowater, Richard; Doherty, Aidan J.
- PLoS Genetics, Vol. 2, Issue 2
Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
journal, September 2012
- Gasiunas, G.; Barrangou, R.; Horvath, P.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 39, p. E2579-E2586
CRISPR Interference Can Prevent Natural Transformation and Virulence Acquisition during In Vivo Bacterial Infection
journal, August 2012
- Bikard, David; Hatoum-Aslan, Asma; Mucida, Daniel
- Cell Host & Microbe, Vol. 12, Issue 2, p. 177-186
Genome editing of Clostridium autoethanogenum using CRISPR/Cas9
journal, October 2016
- Nagaraju, Shilpa; Davies, Naomi Kathleen; Walker, David Jeffrey Fraser
- Biotechnology for Biofuels, Vol. 9, Issue 1
CRISPR/Cas9-Based Efficient Genome Editing in Clostridium ljungdahlii , an Autotrophic Gas-Fermenting Bacterium
journal, June 2016
- Huang, He; Chai, Changsheng; Li, Ning
- ACS Synthetic Biology, Vol. 5, Issue 12
Genome Engineering of Eubacterium limosum Using Expanded Genetic Tools and the CRISPR-Cas9 System
journal, August 2019
- Shin, Jongoh; Kang, Seulgi; Song, Yoseb
- ACS Synthetic Biology, Vol. 8, Issue 9
The CRISPRdb database and tools to display CRISPRs and to generate dictionaries of spacers and repeats
journal, January 2007
- Grissa, Ibtissem; Vergnaud, Gilles; Pourcel, Christine
- BMC Bioinformatics, Vol. 8, Issue 1
Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium
journal, May 2016
- Pyne, Michael E.; Bruder, Mark R.; Moo-Young, Murray
- Scientific Reports, Vol. 6, Issue 1
Using an Endogenous CRISPR-Cas System for Genome Editing in the Human Pathogen Clostridium difficile
journal, August 2019
- Maikova, Anna; Kreis, Victor; Boutserin, Anaïs
- Applied and Environmental Microbiology, Vol. 85, Issue 20
Exploiting endogenous CRISPR-Cas system for multiplex genome editing in Clostridium tyrobutyricum and engineer the strain for high-level butanol production
journal, May 2018
- Zhang, Jie; Zong, Wenming; Hong, Wei
- Metabolic Engineering, Vol. 47
DNA targeting specificity of RNA-guided Cas9 nucleases
journal, July 2013
- Hsu, Patrick D.; Scott, David A.; Weinstein, Joshua A.
- Nature Biotechnology, Vol. 31, Issue 9, p. 827-832
Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii
journal, September 2014
- Jiang, Wenzhi; Brueggeman, Andrew J.; Horken, Kempton M.
- Eukaryotic Cell, Vol. 13, Issue 11
Metabolic load and heterologous gene expression
journal, January 1995
- Glick, Bernard R.
- Biotechnology Advances, Vol. 13, Issue 2
CRISPR interference (CRISPRi) for sequence-specific control of gene expression
journal, October 2013
- Larson, Matthew H.; Gilbert, Luke A.; Wang, Xiaowo
- Nature Protocols, Vol. 8, Issue 11
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol
journal, January 2008
- Liu, Huanting; Naismith, James H.
- BMC Biotechnology, Vol. 8, Issue 1
Why are essential genes essential? - The essentiality of Saccharomyces genes
journal, July 2015
- Zhang, Zhaojie; Ren, Qun
- Microbial Cell, Vol. 2, Issue 8
Construction of a Gene Knockdown System Based on Catalytically Inactive (“Dead”) Cas9 (dCas9) in Staphylococcus aureus
journal, June 2017
- Zhao, Changlong; Shu, Xueqin; Sun, Baolin
- Applied and Environmental Microbiology, Vol. 83, Issue 12
Transcriptional control of Clostridium autoethanogenum using CRISPRi
journal, January 2021
- Fackler, Nicholas; Heffernan, James; Juminaga, Alex
- Synthetic Biology, Vol. 6, Issue 1
Rediverting carbon flux in Clostridium ljungdahlii using CRISPR interference (CRISPRi)
journal, July 2018
- Woolston, Benjamin M.; Emerson, David F.; Currie, Devin H.
- Metabolic Engineering, Vol. 48
Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression
journal, October 2014
- Luo, Michelle L.; Mullis, Adam S.; Leenay, Ryan T.
- Nucleic Acids Research, Vol. 43, Issue 1
A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria
journal, June 2016
- Peters, Jason M.; Colavin, Alexandre; Shi, Handuo
- Cell, Vol. 165, Issue 6
Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance
journal, June 2018
- Wang, Tianmin; Guan, Changge; Guo, Jiahui
- Nature Communications, Vol. 9, Issue 1
A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
journal, May 2018
- Cui, Lun; Vigouroux, Antoine; Rousset, François
- Nature Communications, Vol. 9, Issue 1
Simultaneous repression of multiple bacterial genes using nonrepetitive extra-long sgRNA arrays
journal, October 2019
- Reis, Alexander C.; Halper, Sean M.; Vezeau, Grace E.
- Nature Biotechnology, Vol. 37, Issue 11
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
journal, October 2015
- Zetsche, Bernd; Gootenberg, Jonathan S.; Abudayyeh, Omar O.
- Cell, Vol. 163, Issue 3, p. 759-771
Cas9 versus Cas12a/Cpf1: Structure-function comparisons and implications for genome editing
journal, May 2018
- Swarts, Daan C.; Jinek, Martin
- Wiley Interdisciplinary Reviews: RNA, Vol. 9, Issue 5
CasPDB: an integrated and annotated database for Cas proteins from bacteria and archaea
journal, January 2019
- Tang, Zhongjie; Chen, ShaoQi; Chen, Ang
- Database, Vol. 2019
CRISPR-Cas12a-Mediated Gene Deletion and Regulation in Clostridium ljungdahlii and Its Application in Carbon Flux Redirection in Synthesis Gas Fermentation
journal, September 2019
- Zhao, Ran; Liu, Yanqiang; Zhang, Huan
- ACS Synthetic Biology, Vol. 8, Issue 10
Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array
journal, December 2016
- Zetsche, Bernd; Heidenreich, Matthias; Mohanraju, Prarthana
- Nature Biotechnology, Vol. 35, Issue 1
Building a global alliance of biofoundries
journal, May 2019
- Hillson, Nathan; Caddick, Mark; Cai, Yizhi
- Nature Communications, Vol. 10, Issue 1
Rapid Generation of Universal Synthetic Promoters for Controlled Gene Expression in Both Gas-Fermenting and Saccharolytic Clostridium Species
journal, June 2017
- Yang, Gaohua; Jia, Dechen; Jin, Lin
- ACS Synthetic Biology, Vol. 6, Issue 9
Stringency of Synthetic Promoter Sequences in Clostridium Revealed and Circumvented by Tuning Promoter Library Mutation Rates
journal, January 2018
- Mordaka, Paweł M.; Heap, John T.
- ACS Synthetic Biology, Vol. 7, Issue 2
Cell-free gene expression: an expanded repertoire of applications
journal, November 2019
- Silverman, Adam D.; Karim, Ashty S.; Jewett, Michael C.
- Nature Reviews Genetics, Vol. 21, Issue 3
Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System
journal, December 2013
- Sun, Zachary Z.; Yeung, Enoch; Hayes, Clarmyra A.
- ACS Synthetic Biology, Vol. 3, Issue 6
Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology
journal, January 2013
- Chappell, James; Jensen, Kirsten; Freemont, Paul S.
- Nucleic Acids Research, Vol. 41, Issue 5
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System
journal, January 2018
- Marshall, Ryan; Maxwell, Colin S.; Collins, Scott P.
- Molecular Cell, Vol. 69, Issue 1
Cell-free prototyping strategies for enhancing the sustainable production of polyhydroxyalkanoates bioplastics
journal, January 2018
- Kelwick, Richard; Ricci, Luca; Chee, Soo Mei
- Synthetic Biology, Vol. 3, Issue 1
Modular cell-free expression plasmids to accelerate biological design in cells
journal, January 2020
- Karim, Ashty S.; Liew, Fungmin (Eric); Garg, Shivani
- Synthetic Biology, Vol. 5, Issue 1
Development of a clostridia-based cell-free system for prototyping genetic parts and metabolic pathways
journal, November 2020
- Krüger, Antje; Mueller, Alexander P.; Rybnicky, Grant A.
- Metabolic Engineering, Vol. 62
Cell-free protein synthesis using cell extract of Pseudomonas fluorescens and CspA promoter
journal, June 2004
- Nakashima, Nobutaka; Tamura, Tomohiro
- Biochemical and Biophysical Research Communications, Vol. 319, Issue 2
Development of a Pseudomonas putida cell-free protein synthesis platform for rapid screening of gene regulatory elements
journal, January 2018
- Wang, He; Li, Jian; Jewett, Michael C.
- Synthetic Biology, Vol. 3, Issue 1
Establishing a high yielding streptomyces -based cell-free protein synthesis system: Establishing a Streptomyces -Based CFPS System
journal, February 2017
- Li, Jian; Wang, He; Kwon, Yong-Chan
- Biotechnology and Bioengineering, Vol. 114, Issue 6
Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool
journal, February 2017
- Moore, Simon J.; Lai, Hung-En; Needham, Hannah
- Biotechnology Journal, Vol. 12, Issue 4
Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens
journal, August 2018
- Des Soye, Benjamin J.; Davidson, Samuel R.; Weinstock, Matthew T.
- ACS Synthetic Biology, Vol. 7, Issue 9
Rapid acquisition and model-based analysis of cell-free transcription–translation reactions from nonmodel bacteria
journal, April 2018
- Moore, Simon J.; MacDonald, James T.; Wienecke, Sarah
- Proceedings of the National Academy of Sciences, Vol. 115, Issue 19
Multiplex transcriptional characterizations across diverse bacterial species using cell‐free systems
journal, August 2019
- Yim, Sung Sun; Johns, Nathan I.; Park, Jimin
- Molecular Systems Biology, Vol. 15, Issue 8
An Integrated Multi-Disciplinary Perspective for Addressing Challenges of the Human Gut Microbiome
journal, March 2020
- Shah, Rohan M.; McKenzie, Elizabeth J.; Rosin, Magda T.
- Metabolites, Vol. 10, Issue 3
H2 drives metabolic rearrangements in gas-fermenting Clostridium autoethanogenum
journal, March 2018
- Valgepea, Kaspar; de Souza Pinto Lemgruber, Renato; Abdalla, Tanus
- Biotechnology for Biofuels, Vol. 11, Issue 1
Ethanol production in syngas-fermenting Clostridium ljungdahlii is controlled by thermodynamics rather than by enzyme expression
journal, January 2016
- Richter, H.; Molitor, B.; Wei, H.
- Energy & Environmental Science, Vol. 9, Issue 7
Arginine deiminase pathway provides ATP and boosts growth of the gas-fermenting acetogen Clostridium autoethanogenum
journal, May 2017
- Valgepea, Kaspar; Loi, Kim Q.; Behrendorff, James B.
- Metabolic Engineering, Vol. 41
Metabolic kinetic modeling provides insight into complex biological questions, but hurdles remain
journal, October 2019
- Strutz, Jonathan; Martin, Jacob; Greene, Jennifer
- Current Opinion in Biotechnology, Vol. 59
Acceleration Strategies to Enhance Metabolic Ensemble Modeling Performance
journal, September 2017
- Greene, Jennifer L.; Wäechter, Andreas; Tyo, Keith E. J.
- Biophysical Journal, Vol. 113, Issue 5
Testing Biochemistry Revisited: How In Vivo Metabolism Can Be Understood from In Vitro Enzyme Kinetics
journal, April 2012
- van Eunen, Karen; Kiewiet, José A. L.; Westerhoff, Hans V.
- PLoS Computational Biology, Vol. 8, Issue 4
Kinetic ensemble model of gas fermenting Clostridium autoethanogenum for improved ethanol production
journal, August 2019
- Greene, Jennifer; Daniell, James; Köpke, Michael
- Biochemical Engineering Journal, Vol. 148
Predicting proteome allocation, overflow metabolism, and metal requirements in a model acetogen
journal, March 2019
- Liu, Joanne K.; Lloyd, Colton; Al-Bassam, Mahmoud M.
- PLOS Computational Biology, Vol. 15, Issue 3
Large-scale kinetic metabolic models of Pseudomonas putida KT2440 for consistent design of metabolic engineering strategies
journal, February 2020
- Tokic, Milenko; Hatzimanikatis, Vassily; Miskovic, Ljubisa
- Biotechnology for Biofuels, Vol. 13, Issue 1
Ensemble Modeling of Metabolic Networks
journal, December 2008
- Tran, Linh M.; Rizk, Matthew L.; Liao, James C.
- Biophysical Journal, Vol. 95, Issue 12
Toward a genome scale sequence specific dynamic model of cell-free protein synthesis in Escherichia coli
journal, June 2020
- Horvath, Nicholas; Vilkhovoy, Michael; Wayman, Joseph A.
- Metabolic Engineering Communications, Vol. 10
Exploring biochemical pathways for mono-ethylene glycol (MEG) synthesis from synthesis gas
journal, May 2017
- Islam, M. Ahsanul; Hadadi, Noushin; Ataman, Meric
- Metabolic Engineering, Vol. 41
Design and analysis of metabolic pathways supporting formatotrophic growth for electricity-dependent cultivation of microbes
journal, August 2013
- Bar-Even, Arren; Noor, Elad; Flamholz, Avi
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 8-9
Formate Assimilation: The Metabolic Architecture of Natural and Synthetic Pathways
journal, July 2016
- Bar-Even, Arren
- Biochemistry, Vol. 55, Issue 28
A synthetic pathway for the fixation of carbon dioxide in vitro
journal, November 2016
- Schwander, Thomas; Schada von Borzyskowski, Lennart; Burgener, Simon
- Science, Vol. 354, Issue 6314
The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans
journal, October 2020
- Sánchez-Andrea, Irene; Guedes, Iame Alves; Hornung, Bastian
- Nature Communications, Vol. 11, Issue 1
Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia coli
journal, December 2016
- Sévin, Daniel C.; Fuhrer, Tobias; Zamboni, Nicola
- Nature Methods, Vol. 14, Issue 2
Computational discovery of biochemical routes to specialty chemicals
journal, November 2004
- Li, Chunhui; Henry, Christopher S.; Jankowski, Matthew D.
- Chemical Engineering Science, Vol. 59, Issue 22-23
MINEs: open access databases of computationally predicted enzyme promiscuity products for untargeted metabolomics
journal, August 2015
- Jeffryes, James G.; Colastani, Ricardo L.; Elbadawi-Sidhu, Mona
- Journal of Cheminformatics, Vol. 7, Issue 1
Characterizing and ranking computed metabolic engineering strategies
journal, January 2019
- Schneider, Philipp; Klamt, Steffen
- Bioinformatics, Vol. 35, Issue 17
Exploring De Novo metabolic pathways from pyruvate to propionic acid
journal, March 2016
- Stine, Andrew; Zhang, Miaomin; Ro, Soo
- Biotechnology Progress, Vol. 32, Issue 2
Evaluating enzymatic synthesis of small molecule drugs
journal, January 2016
- Moura, Matthew; Finkle, Justin; Stainbrook, Sarah
- Metabolic Engineering, Vol. 33
Consistent Estimation of Gibbs Energy Using Component Contributions
journal, July 2013
- Noor, Elad; Haraldsdóttir, Hulda S.; Milo, Ron
- PLoS Computational Biology, Vol. 9, Issue 7
Pathway Thermodynamics Highlights Kinetic Obstacles in Central Metabolism
journal, February 2014
- Noor, Elad; Bar-Even, Arren; Flamholz, Avi
- PLoS Computational Biology, Vol. 10, Issue 2
Genome-scale model of C. autoethanogenum reveals optimal bioprocessconditions for high-value chemical production from carbon monoxide
journal, June 2019
- Norman, Rupert O. J.; Millat, Thomas; Schatschneider, Sarah
- Engineering Biology, Vol. 3, Issue 2
Progress towards platform chemical production using Clostridium autoethanogenum
journal, April 2018
- Norman, Rupert O. J.; Millat, Thomas; Winzer, Klaus
- Biochemical Society Transactions, Vol. 46, Issue 3
Efficient searching and annotation of metabolic networks using chemical similarity
journal, December 2014
- Pertusi, Dante A.; Stine, Andrew E.; Broadbelt, Linda J.
- Bioinformatics, Vol. 31, Issue 7
Power-to-protein: converting renewable electric power and carbon dioxide into single cell protein with a two-stage bioprocess
journal, January 2019
- Molitor, Bastian; Mishra, Akanksha; Angenent, Largus T.
- Energy & Environmental Science, Vol. 12, Issue 12
Microbial Electrosynthesis: Feeding Microbes Electricity To Convert Carbon Dioxide and Water to Multicarbon Extracellular Organic Compounds
journal, May 2010
- Nevin, K. P.; Woodard, T. L.; Franks, A. E.
- mBio, Vol. 1, Issue 2, Article No. e00103-10
Electrosynthesis of Organic Compounds from Carbon Dioxide Is Catalyzed by a Diversity of Acetogenic Microorganisms
journal, March 2011
- Nevin, Kelly P.; Hensley, Sarah A.; Franks, Ashley E.
- Applied and Environmental Microbiology, Vol. 77, Issue 9, p. 2882-2886