Metagenomic and metatranscriptomic analysis reveals enrichment for xenobiotic‐degrading bacterial specialists and xenobiotic‐degrading genes in a Canadian Prairie two‐cell biobed system

Russell, Jennifer N.; Perry, Benjamin J.; Bergsveinson, Jordyn; Freeman, Claire N.; Sheedy, Claudia; Nilsson, Denise; Braul, Larry; Yost, Christopher K.

doi:10.1111/1758-2229.12990

Title: Metagenomic and metatranscriptomic analysis reveals enrichment for xenobiotic‐degrading bacterial specialists and xenobiotic‐degrading genes in a Canadian Prairie two‐cell biobed system

Journal Article · 07 July 2021 · Environmental Microbiology Reports

DOI: https://doi.org/10.1111/1758-2229.12990 · OSTI ID:1806365

Russell, Jennifer N. ^[1]; Perry, Benjamin J. ^[1]; Bergsveinson, Jordyn ^[2];

^[1]; Sheedy, Claudia ^[3]; Nilsson, Denise ^[3]; Braul, Larry ^[4];

^[1]

Department of Biology University of Regina Regina S4S 0A2 Canada
National Hydrology Research Centre Environment Canada and Climate Change Canada Saskatoon S7N 3H5 Canada
Lethbridge Research and Development Centre Agriculture and Agri‐Food Canada Lethbridge T1J 4B1 Canada
Research Branch Agriculture and Agri‐Food Canada Regina S4P 0M3 Canada

Summary Biobeds are agriculture‐based bioremediation tools used to safely contain and microbially degrade on‐farm pesticide waste and rinsate, thereby reducing the negative environmental impacts associated with pesticide use. While these engineered ecosystems demonstrate efficient pesticide removal, the microbiomes in these environments remain largely understudied both taxonomically and functionally. This study used metagenomic and metatranscriptomic techniques to characterize the microbial community in a two‐cell Canadian biobed system before and after a field season of pesticide application. These culture‐independent approaches identified an enrichment of xenobiotic‐degrading bacteria, such as Afipia , Sphingopyxis and Pseudomonas , and enrichment and transcription of xenobiotic‐degrading genes, such as peroxidases, oxygenases, and hydroxylases, among others; we were able to directly link the transcription of these genes to Pseudomonas , Oligotropha , Mesorhizobium , Rhodopseudomonas , and Stenotrophomonas taxa.

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Sponsoring Organization:: USDOE

OSTI ID:: 1806365

Journal Information:: Environmental Microbiology Reports, Journal Name: Environmental Microbiology Reports Journal Issue: 5 Vol. 13; ISSN 1758-2229

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United States

Language:: English

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