Improved chemical pulping and saccharification of a natural mulberry mutant deficient in cinnamyl alcohol dehydrogenase
Abstract
Lignin content and its molecular structure influence various wood characteristics. In this study, the anatomical and physicochemical properties of wood derived from a naturally occurring mulberry mutant deficient in cinnamyl alcohol dehydrogenase (CAD), a key enzyme in lignin biosynthesis, were analyzed using conventional staining assays on stem sections, length and width measurements of xylem fiber cells, wood pulping and saccharification assays, and sugar compositional analysis of extractive-free wood powder. Here, the present data indicate that the mutation in the CAD gene leads to improved wood delignification efficiency, increased pulp yield under alkaline pulping conditions, and enhanced saccharification efficiency following alkaline pretreatment. This study opens up new avenues for the multipurpose use of the mulberry CAD-deficient mutant as a raw material for biorefinery processes, in addition to its traditional use as a favored feed for silkworms.
- Authors:
-
[1];
[2];
[2];
[5];
[4];
[3]
- Forest Research and Management Organization, Ibaraki (Japan)
- National Inst. of Advanced Industrial Science and Technology (AIST), Ibaraki (Japan)
- Tokyo Univ. of Agriculture and Technology (Japan)
- Univ. of Wisconsin, Madison, WI (United States); Wisconsin Energy Inst., Madison, WI (United States)
- VIB, Ghent (Belgium); Ghent Univ. (Belgium)
- Publication Date:
- Research Org.:
- Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1804440
- Grant/Contract Number:
- SC0018409
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Holzforschung
- Additional Journal Information:
- Journal Volume: 75; Journal Issue: 10; Journal ID: ISSN 0018-3830
- Publisher:
- de Gruyter
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; alkaline pulping; cell wall; enzyme saccharification; lignin; lignocellulose
Citation Formats
Ikeda, Tsutomu, Takata, Naoki, Sakamoto, Shingo, Hu, Shi, Nuoendagula, None, Hishiyama, Shojiro, Mitsuda, Nobutaka, Boerjan, Wout, Ralph, John, and Kajita, Shinya. Improved chemical pulping and saccharification of a natural mulberry mutant deficient in cinnamyl alcohol dehydrogenase. United States: N. p., 2021.
Web. doi:10.1515/hf-2021-0015.
Ikeda, Tsutomu, Takata, Naoki, Sakamoto, Shingo, Hu, Shi, Nuoendagula, None, Hishiyama, Shojiro, Mitsuda, Nobutaka, Boerjan, Wout, Ralph, John, & Kajita, Shinya. Improved chemical pulping and saccharification of a natural mulberry mutant deficient in cinnamyl alcohol dehydrogenase. United States. https://doi.org/10.1515/hf-2021-0015
Ikeda, Tsutomu, Takata, Naoki, Sakamoto, Shingo, Hu, Shi, Nuoendagula, None, Hishiyama, Shojiro, Mitsuda, Nobutaka, Boerjan, Wout, Ralph, John, and Kajita, Shinya. Thu .
"Improved chemical pulping and saccharification of a natural mulberry mutant deficient in cinnamyl alcohol dehydrogenase". United States. https://doi.org/10.1515/hf-2021-0015. https://www.osti.gov/servlets/purl/1804440.
@article{osti_1804440,
title = {Improved chemical pulping and saccharification of a natural mulberry mutant deficient in cinnamyl alcohol dehydrogenase},
author = {Ikeda, Tsutomu and Takata, Naoki and Sakamoto, Shingo and Hu, Shi and Nuoendagula, None and Hishiyama, Shojiro and Mitsuda, Nobutaka and Boerjan, Wout and Ralph, John and Kajita, Shinya},
abstractNote = {Lignin content and its molecular structure influence various wood characteristics. In this study, the anatomical and physicochemical properties of wood derived from a naturally occurring mulberry mutant deficient in cinnamyl alcohol dehydrogenase (CAD), a key enzyme in lignin biosynthesis, were analyzed using conventional staining assays on stem sections, length and width measurements of xylem fiber cells, wood pulping and saccharification assays, and sugar compositional analysis of extractive-free wood powder. Here, the present data indicate that the mutation in the CAD gene leads to improved wood delignification efficiency, increased pulp yield under alkaline pulping conditions, and enhanced saccharification efficiency following alkaline pretreatment. This study opens up new avenues for the multipurpose use of the mulberry CAD-deficient mutant as a raw material for biorefinery processes, in addition to its traditional use as a favored feed for silkworms.},
doi = {10.1515/hf-2021-0015},
journal = {Holzforschung},
number = 10,
volume = 75,
place = {United States},
year = {Thu May 13 00:00:00 EDT 2021},
month = {Thu May 13 00:00:00 EDT 2021}
}
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