Overexpression of a Sugarcane BAHD Acyltransferase Alters Hydroxycinnamate Content in Maize Cell Wall
Abstract
The purification of hydroxycinnamic acids [p-coumaric acid (pCA) and ferulic acid (FA)] from grass cell walls requires high-cost processes. Feedstocks with increased levels of one hydroxycinnamate in preference to the other are therefore highly desirable. We identified and conducted expression analysis for nine BAHD acyltransferase ScAts genes from sugarcane. The high conservation of AT10 proteins, together with their similar gene expression patterns, supported a similar role in distinct grasses. Overexpression of ScAT10 in maize resulted in up to 75% increase in total pCA content. Mild hydrolysis and derivatization followed by reductive cleavage (DFRC) analysis showed that pCA increase was restricted to the hemicellulosic portion of the cell wall. Furthermore, total FA content was reduced up to 88%, resulting in a 10-fold increase in the pCA/FA ratio. Thus, we functionally characterized a sugarcane gene involved in pCA content on hemicelluloses and generated a C4 plant that is promising for valorizing pCA production in biorefineries.
- Authors:
-
- Univ. of Sao Paulo (Brazil)
- Lytic Solutions, LLC, Madison, WI (United States)
- US Dept. of Agriculture (USDA), Madison, WI (United States)
- Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States); Univ. of Wisconsin, Madison, WI (United States)
- Univ. of Campinas (UNICAMP), Sao Paulo (Brazil)
- Publication Date:
- Research Org.:
- Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); São Paulo Research Foundation (FAPESP); National Council for Scientific and Technological Development (CNPq); USP International Cooperation Office (AUCANI); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
- OSTI Identifier:
- 1804112
- Grant/Contract Number:
- SC0018409; 2014/17486-6; 2016/24391-7; 2021/02352-8; 14/2014; 527/2016; 2014/06923-6; 2016/25785-9; 142474/2015-0; 88881.135309/2016-01
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Plant Science
- Additional Journal Information:
- Journal Volume: 12; Journal ID: ISSN 1664-462X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; BAHD acyltransferases; biomass engineering; biorefineries; ferulic acid (FA); p-coumaric acid (pCA)
Citation Formats
Fanelli, Amanda, Rancour, David M., Sullivan, Michael, Karlen, Steven D., Ralph, John, Riaño-Pachón, Diego Mauricio, Vicentini, Renato, Silva, Tatiane Franca, Ferraz, André, Hatfield, Ronald D., and Romanel, Elisson. Overexpression of a Sugarcane BAHD Acyltransferase Alters Hydroxycinnamate Content in Maize Cell Wall. United States: N. p., 2021.
Web. doi:10.3389/fpls.2021.626168.
Fanelli, Amanda, Rancour, David M., Sullivan, Michael, Karlen, Steven D., Ralph, John, Riaño-Pachón, Diego Mauricio, Vicentini, Renato, Silva, Tatiane Franca, Ferraz, André, Hatfield, Ronald D., & Romanel, Elisson. Overexpression of a Sugarcane BAHD Acyltransferase Alters Hydroxycinnamate Content in Maize Cell Wall. United States. https://doi.org/10.3389/fpls.2021.626168
Fanelli, Amanda, Rancour, David M., Sullivan, Michael, Karlen, Steven D., Ralph, John, Riaño-Pachón, Diego Mauricio, Vicentini, Renato, Silva, Tatiane Franca, Ferraz, André, Hatfield, Ronald D., and Romanel, Elisson. Wed .
"Overexpression of a Sugarcane BAHD Acyltransferase Alters Hydroxycinnamate Content in Maize Cell Wall". United States. https://doi.org/10.3389/fpls.2021.626168. https://www.osti.gov/servlets/purl/1804112.
@article{osti_1804112,
title = {Overexpression of a Sugarcane BAHD Acyltransferase Alters Hydroxycinnamate Content in Maize Cell Wall},
author = {Fanelli, Amanda and Rancour, David M. and Sullivan, Michael and Karlen, Steven D. and Ralph, John and Riaño-Pachón, Diego Mauricio and Vicentini, Renato and Silva, Tatiane Franca and Ferraz, André and Hatfield, Ronald D. and Romanel, Elisson},
abstractNote = {The purification of hydroxycinnamic acids [p-coumaric acid (pCA) and ferulic acid (FA)] from grass cell walls requires high-cost processes. Feedstocks with increased levels of one hydroxycinnamate in preference to the other are therefore highly desirable. We identified and conducted expression analysis for nine BAHD acyltransferase ScAts genes from sugarcane. The high conservation of AT10 proteins, together with their similar gene expression patterns, supported a similar role in distinct grasses. Overexpression of ScAT10 in maize resulted in up to 75% increase in total pCA content. Mild hydrolysis and derivatization followed by reductive cleavage (DFRC) analysis showed that pCA increase was restricted to the hemicellulosic portion of the cell wall. Furthermore, total FA content was reduced up to 88%, resulting in a 10-fold increase in the pCA/FA ratio. Thus, we functionally characterized a sugarcane gene involved in pCA content on hemicelluloses and generated a C4 plant that is promising for valorizing pCA production in biorefineries.},
doi = {10.3389/fpls.2021.626168},
journal = {Frontiers in Plant Science},
number = ,
volume = 12,
place = {United States},
year = {Wed Apr 21 00:00:00 EDT 2021},
month = {Wed Apr 21 00:00:00 EDT 2021}
}
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