Reaction centers of the thermophilic microaerophile, Chloracidobacterium thermophilum (Acidobacteria) I: biochemical and biophysical characterization
Abstract
Chloracidobacterium thermophilum is a microaerophilic, anoxygenic member of the green chlorophototrophic bacteria. This bacterium is the first characterized oxygen-requiring chlorophototroph with chlorosomes, the FMO protein, and homodimeric type-1 reaction centers (RCs). The RCs of C. thermophilum are also unique because they contain three types of chlorophylls, bacteriochlorophyll aP esterified with phytol, Chl aPD esterified with Δ2,6-phytadienol, and Zn-BChl aP' esterified with phytol, in the approximate molar ratio 32:24:4. The light-induced difference spectrum of these RCs had a bleaching maximum at 839 nm and also revealed an electrochromic bandshift that is probably derived from a BChl a molecule near P840+. The FX [4Fe–4S] cluster had a midpoint potential of ca. - 581 mV, and the spectroscopic properties of the P+ F$$^-_X$$ spin-polarized radical pair were very similar to those of reaction centers of heliobacteria and green sulfur bacteria. The data further indicate that electron transfer occurs directly from A0- to FX, as occurs in other homodimeric type-1 RCs. Washing experiments with isolated membranes suggested that the PscB subunit of these reaction centers is more tightly bound than PshB in heliobacteria. Thus, the reaction centers of C. thermophilum have some properties that resemble other homodimeric reaction centers but also have specific properties that are more similar to those of Photosystem I. These differences likely contribute to protection of the electron transfer chain from oxygen, contributing to the oxygen tolerance of this microaerophile.
- Authors:
-
[2]
- Pennsylvania State Univ., University Park, PA (United States)
- Pennsylvania State Univ., University Park, PA (United States); Montana State Univ., Bozeman, MT (United States)
- Publication Date:
- Research Org.:
- Pennsylvania State Univ., University Park, PA (United States); Arizona State Univ., Tempe, AZ (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1800808
- Grant/Contract Number:
- FG02-94ER20137; SC0010575
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Photosynthesis Research
- Additional Journal Information:
- Journal Volume: 142; Journal Issue: 1; Journal ID: ISSN 0166-8595
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Plant Sciences
Citation Formats
He, Zhihui, Ferlez, Bryan, Kurashov, Vasily, Tank, Marcus, Golbeck, John H., and Bryant, Donald A. Reaction centers of the thermophilic microaerophile, Chloracidobacterium thermophilum (Acidobacteria) I: biochemical and biophysical characterization. United States: N. p., 2019.
Web. doi:10.1007/s11120-019-00650-9.
He, Zhihui, Ferlez, Bryan, Kurashov, Vasily, Tank, Marcus, Golbeck, John H., & Bryant, Donald A. Reaction centers of the thermophilic microaerophile, Chloracidobacterium thermophilum (Acidobacteria) I: biochemical and biophysical characterization. United States. https://doi.org/10.1007/s11120-019-00650-9
He, Zhihui, Ferlez, Bryan, Kurashov, Vasily, Tank, Marcus, Golbeck, John H., and Bryant, Donald A. Mon .
"Reaction centers of the thermophilic microaerophile, Chloracidobacterium thermophilum (Acidobacteria) I: biochemical and biophysical characterization". United States. https://doi.org/10.1007/s11120-019-00650-9. https://www.osti.gov/servlets/purl/1800808.
@article{osti_1800808,
title = {Reaction centers of the thermophilic microaerophile, Chloracidobacterium thermophilum (Acidobacteria) I: biochemical and biophysical characterization},
author = {He, Zhihui and Ferlez, Bryan and Kurashov, Vasily and Tank, Marcus and Golbeck, John H. and Bryant, Donald A.},
abstractNote = {Chloracidobacterium thermophilum is a microaerophilic, anoxygenic member of the green chlorophototrophic bacteria. This bacterium is the first characterized oxygen-requiring chlorophototroph with chlorosomes, the FMO protein, and homodimeric type-1 reaction centers (RCs). The RCs of C. thermophilum are also unique because they contain three types of chlorophylls, bacteriochlorophyll aP esterified with phytol, Chl aPD esterified with Δ2,6-phytadienol, and Zn-BChl aP' esterified with phytol, in the approximate molar ratio 32:24:4. The light-induced difference spectrum of these RCs had a bleaching maximum at 839 nm and also revealed an electrochromic bandshift that is probably derived from a BChl a molecule near P840+. The FX [4Fe–4S] cluster had a midpoint potential of ca. - 581 mV, and the spectroscopic properties of the P+ F$^-_X$ spin-polarized radical pair were very similar to those of reaction centers of heliobacteria and green sulfur bacteria. The data further indicate that electron transfer occurs directly from A0- to FX, as occurs in other homodimeric type-1 RCs. Washing experiments with isolated membranes suggested that the PscB subunit of these reaction centers is more tightly bound than PshB in heliobacteria. Thus, the reaction centers of C. thermophilum have some properties that resemble other homodimeric reaction centers but also have specific properties that are more similar to those of Photosystem I. These differences likely contribute to protection of the electron transfer chain from oxygen, contributing to the oxygen tolerance of this microaerophile.},
doi = {10.1007/s11120-019-00650-9},
journal = {Photosynthesis Research},
number = 1,
volume = 142,
place = {United States},
year = {Mon Jun 03 00:00:00 EDT 2019},
month = {Mon Jun 03 00:00:00 EDT 2019}
}
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