Microbial communities from Arctic marine sediments respond slowly to methane addition during ex situ enrichments

Klasek, Scott; Torres, Marta E.; Bartlett, Douglas H.; Tyler, Madeline; Hong, Wei‐Li; Colwell, Frederick

doi:10.1111/1462-2920.14895

Title: Microbial communities from Arctic marine sediments respond slowly to methane addition during ex situ enrichments

Journal Article · 14 December 2019 · Environmental Microbiology

DOI: https://doi.org/10.1111/1462-2920.14895 · OSTI ID:1799761

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^[3]; Tyler, Madeline ^[2];

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Oregon State Univ., Corvallis, OR (United States); OSTI
Oregon State Univ., Corvallis, OR (United States)
Univ. of California, San Diego, CA (United States). Scripps Inst. of Oceanography
UiT The Arctic Univ. of Norway, Tromso (Norway)

Anaerobic methanotrophic archaea (ANME) consume methane in marine sediments, limiting its release to the water column, but their responses to changes in methane and sulfate supplies remain poorly constrained. To address how methane exposure may affect microbial communities and methane- and sulfur-cycling gene abundances in Arctic marine sediments, in this study we collected sediments from offshore Svalbard that represent geochemical horizons where anaerobic methanotrophy is expected to be active, previously active, and long-inactive based on reaction-transport biogeochemical modelling of porewater sulfate profiles. Sediment slurries were incubated at in situ temperature and pressure with different added methane concentrations. Sediments from an active area of seepage began to reduce sulfate in a methane-dependent manner within months, preceding increased relative abundances of anaerobic methanotrophs ANME-1 within communities. In previously active and long-inactive sediments, sulfur-cycling Deltaproteobacteria became more dominant after 30 days, though these communities showed no evidence of methanotrophy after nearly 8 months of enrichment. Overall, enrichment conditions, but not methane, broadly altered microbial community structure across different enrichment times and sediment types. These results suggest that active ANME populations may require years to develop, and consequently microbial community composition may affect methanotrophic responses to potential large-scale seafloor methane releases in ways that provide insight for future modelling studies.

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Research Organization:: Oregon State Univ., Corvallis, OR (United States)

Sponsoring Organization:: Research Council of Norway (RCN); USDOE; USDOE Office of Fossil Energy (FE)

Grant/Contract Number:: FE0013531

OSTI ID:: 1799761

Journal Information:: Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 5 Vol. 22; ISSN 1462-2912

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
AOM
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pressure enrichment
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Title: Microbial communities from Arctic marine sediments respond slowly to methane addition during ex situ enrichments

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