Title: The application of flow cytometry for kelp meiospore isolation

Journal Article · · Algal Research
 [1];  [2];  [2];  [3];  [3];  [4];  [4];  [3]
  1. Univ. of Connecticut, Storrs, CT (United States); Symbrosia LLC., Kailua-Kona, HI (United States); OSTI
  2. NOAA Northeast Marine Fisheries Science Center, Milford, CT (United States)
  3. Univ. of Connecticut, Storrs, CT (United States)
  4. Woods Hole Oceanographic Institution, Woods Hole, MA (United States)
+ Show Author Affiliations

The goal of this study was to evaluate flow-cytometric techniques for isolating individual kelp meiospores into 96-welled plates. Previously reported low success rates for sorting tens of thousands of meiospores at a time have been improved by technological developments, specifically, the low nozzle pressure of the commercially available, JSAN (Bay Bioscience Co. Ltd) instrument. We monitored growth and gametophyte development post-sorting for 10 months. Our data demonstrate that successful kelp meiospore isolations of up to 76% viability can be achieved with flow-cytometry. This method can save time as compared to traditional, manual isolations using pipettes and improves confidence that self-fertilized individuals will not contaminate specific crosses of resulting gametophytes. Our results highlight a new application for the flow cytometer to produce clonal kelp gametophytes with direct applications for germplasm and culture collection development.

Research Organization:
Woods Hole Oceanographic Institution, Woods Hole, MA (United States)
Sponsoring Organization:
USDOE; USDOE Advanced Research Projects Agency - Energy (ARPA-E)
Grant/Contract Number:
AR0000915
OSTI ID:
1799111
Journal Information:
Algal Research, Journal Name: Algal Research Vol. 46; ISSN 2211-9264
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English

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59 BASIC BIOLOGICAL SCIENCES
Saccharina
chlorophyll a
cytometry
meiospores
single cell sorting