The F-box protein gene exo - 1 is a target for reverse engineering enzyme hypersecretion in filamentous fungi

Gabriel, Raphael; Thieme, Nils; Liu, Qian; Li, Fangya; Meyer, Lisa T.; Harth, Simon; Jecmenica, Marina; Ramamurthy, Maya; Gorman, Jennifer; Simmons, Blake A.; McCluskey, Kevin; Baker, Scott E.; Tian, Chaoguang; Schuerg, Timo; Singer, Steven W.; Fleißner, André; Benz, J. Philipp

doi:10.1073/pnas.2025689118

Title: The F-box protein gene exo - 1 is a target for reverse engineering enzyme hypersecretion in filamentous fungi

Journal Article · 24 June 2021 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.2025689118 · OSTI ID:1798411

Gabriel, Raphael ^[1];

^[2]; Liu, Qian ^[3];

^[3];

^[2]; Harth, Simon ^[4];

^[4];

^[4]; Gorman, Jennifer ^[4];

^[4];

^[5];

^[6];

^[3]; Schuerg, Timo ^[4];

^[4]; Fleißner, André ^[7];

^[8]

Deconstruction Division, Joint BioEnergy Institute, Emeryville, CA 94608,, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Emeryville, CA 94608,, Institut für Genetik, Technische Universität Braunschweig, 38106 Braunschweig, Germany,
Technical University of Munich School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany,
Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China,
Deconstruction Division, Joint BioEnergy Institute, Emeryville, CA 94608,, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Emeryville, CA 94608,
Fungal Genetics Stock Center, Department of Plant Pathology, Kansas State University, Manhattan, KS 66506,
Deconstruction Division, Joint BioEnergy Institute, Emeryville, CA 94608,, Functional and Systems Biology, Environmental Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352,
Institut für Genetik, Technische Universität Braunschweig, 38106 Braunschweig, Germany,, Braunschweig Integrated Centre of Systems Biology, Technische Universität Braunschweig, 38106 Braunschweig, Germany,
Technical University of Munich School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany,, Institute for Advanced Study, Technical University of Munich, 85748 Garching, Germany

Significance Lignocellulose-based biorefinery relies on plant cell wall degrading enzymes. Current genome editing methods can create fungal enzyme hypersecreter strains by design. However, the identification of candidate genes for targeted engineering of this trait remains a bottleneck, and the necessity of specific inducer molecules further complicates production. By the resequencing of a classical hypersecreting Neurospora crassa mutant, we uncovered that mutation of a gene encoding an F-box protein ( exo - 1 ) causes inducer-independent hypersecretion of amylases, invertase, and pectinases. Systems biology and genetic studies of Δ exo - 1 shed light on the regulation of enzyme secretion in filamentous fungi, allowing targeted reverse engineering of industrially employed fungi, such as Myceliophthora thermophila , demonstrating the power of classical mutants in combination with modern sequencing and omics technologies.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: German Research Foundation (DFG); National Natural Science Foundation of China (NSFC); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1798411

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 26 Vol. 118; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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