Establishment of time‐resolved fluoroimmunoassay of IgG4 based on magnetic microspheres
[1];
- College of Life Sciences and Medicine Zhejiang Sci‐Tech University Hangzhou China
- Tong Ren Hospital Shanghai Jiao Tong University School of Medicine Shanghai China
- College of Life Sciences and Medicine Zhejiang Sci‐Tech University Hangzhou China, College of Pharmacy Zhejiang Chinese Medical University Hangzhou China
- Department of Oncology Wuxi No. 2 Hospital Affiliated to Nanjing Medical University Wuxi China
Abstract Background The abnormal increase in serum IgG4 level is an important clinical symptom of IgG4‐related disease (IgG4‐RD), and the detection of serum IgG4 level is a powerful tool for the diagnosis of IgG4‐RD. This study was conducted to establish a simple and rapid immunoassay for the determination of human serum IgG4 levels. Methods Based on the competition method, a novel immunoassay was established for the determination of human serum IgG4 using a combination of time‐resolved fluoroimmunoassay (TRFIA) and magnetic microspheres. IgG4 was coupled with magnetic microspheres and competed with IgG4 in the samples to bind the Eu 3+ ‐labeled anti‐IgG4 antibody. The immunocomplex was separated and washed in a magnetic field, and the fluorescence counts were measured according to the number of dissociated europium ions. Results The analytical sensitivity of IgG4‐TRFIA based on magnetic microspheres was 0.006 g/L, and the detection range was 0.006–20 g/L under optimal conditions. The precision, recovery, and specificity of this immunoassay were demonstrated to be acceptable. The clinical application of IgG4‐TRFIA based on magnetic microspheres was evaluated and compared with that of immunonephelometry. The results showed that the two detection methods had a good correlation, with a correlation coefficient of .9871. Conclusion IgG4‐TRFIA based on magnetic microspheres has the advantages of high sensitivity, wide detection range, and short analysis time and has the potential to become a useful tool for the diagnosis of IgG4‐RD.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 1788145
- Journal Information:
- Journal of Clinical Laboratory Analysis, Journal Name: Journal of Clinical Laboratory Analysis Journal Issue: 8 Vol. 35; ISSN 0887-8013
- Publisher:
- Wiley Blackwell (John Wiley & Sons)Copyright Statement
- Country of Publication:
- United States
- Language:
- English
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