Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing
Abstract
The sustainability and economic feasibility of modern biorefinery depend on the efficient processing of both carbohydrate and lignin fractions for value-added products. By mimicking the biomass degradation process in white-rote fungi, a tailored two-step fractionation process was developed to maximize the sugar release from switchgrass biomass and to optimize the lignin processability for bioconversion. Biomimicking biomass processing using Formic Acid: Fenton: Organosolv (F 2 O) and achieved high processability for both carbohydrate and lignin. Specifically, switchgrass pretreated by the F 2 O process had 99.6% of the theoretical yield for glucose release. The fractionated lignin was also readily processable by fermentation via Rhodococcus opacus PD630 with a lipid yield of 1.16 g/L. Scanning electron microscope analysis confirmed the fragmentation of switchgrass fiber and the cell wall deconstruction by the F 2 O process. 2D-HSQC NMR further revealed the cleavage of aryl ether linkages (β-O-4) in lignin components. These results revealed the mechanisms for efficient sugar release and lignin bioconversion. The F 2 O process demonstrated effective mimicking of natural biomass utilization system and paved a new path for improving the lignin and carbohydrate processability in next generation lignocellulosic biorefinery.
- Authors:
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
- OSTI Identifier:
- 2325130
- Alternate Identifier(s):
- OSTI ID: 1787972
- Grant/Contract Number:
- EE000612; EE0007104
- Resource Type:
- Published Article
- Journal Name:
- Frontiers in Energy Research
- Additional Journal Information:
- Journal Name: Frontiers in Energy Research Journal Volume: 8; Journal ID: ISSN 2296-598X
- Publisher:
- Frontiers Media SA
- Country of Publication:
- Switzerland
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; lignocellulosic biomass; lipid; organosolv; Fenton; formic acid; pretreatment; biomimicking processing
Citation Formats
Li, Man, Liu, Zhi-Hua, Hao, Naijia, Olson, Michelle L., Li, Qiang, Bhagia, Samarthya, Shinde, Somnath, Kao, Katy C., Ragauskas, Arthur J., Xie, Shangxian, and Yuan, Joshua S. Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing. Switzerland: N. p., 2021.
Web. doi:10.3389/fenrg.2020.00194.
Li, Man, Liu, Zhi-Hua, Hao, Naijia, Olson, Michelle L., Li, Qiang, Bhagia, Samarthya, Shinde, Somnath, Kao, Katy C., Ragauskas, Arthur J., Xie, Shangxian, & Yuan, Joshua S. Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing. Switzerland. https://doi.org/10.3389/fenrg.2020.00194
Li, Man, Liu, Zhi-Hua, Hao, Naijia, Olson, Michelle L., Li, Qiang, Bhagia, Samarthya, Shinde, Somnath, Kao, Katy C., Ragauskas, Arthur J., Xie, Shangxian, and Yuan, Joshua S. Thu .
"Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing". Switzerland. https://doi.org/10.3389/fenrg.2020.00194.
@article{osti_2325130,
title = {Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing},
author = {Li, Man and Liu, Zhi-Hua and Hao, Naijia and Olson, Michelle L. and Li, Qiang and Bhagia, Samarthya and Shinde, Somnath and Kao, Katy C. and Ragauskas, Arthur J. and Xie, Shangxian and Yuan, Joshua S.},
abstractNote = {The sustainability and economic feasibility of modern biorefinery depend on the efficient processing of both carbohydrate and lignin fractions for value-added products. By mimicking the biomass degradation process in white-rote fungi, a tailored two-step fractionation process was developed to maximize the sugar release from switchgrass biomass and to optimize the lignin processability for bioconversion. Biomimicking biomass processing using Formic Acid: Fenton: Organosolv (F 2 O) and achieved high processability for both carbohydrate and lignin. Specifically, switchgrass pretreated by the F 2 O process had 99.6% of the theoretical yield for glucose release. The fractionated lignin was also readily processable by fermentation via Rhodococcus opacus PD630 with a lipid yield of 1.16 g/L. Scanning electron microscope analysis confirmed the fragmentation of switchgrass fiber and the cell wall deconstruction by the F 2 O process. 2D-HSQC NMR further revealed the cleavage of aryl ether linkages (β-O-4) in lignin components. These results revealed the mechanisms for efficient sugar release and lignin bioconversion. The F 2 O process demonstrated effective mimicking of natural biomass utilization system and paved a new path for improving the lignin and carbohydrate processability in next generation lignocellulosic biorefinery.},
doi = {10.3389/fenrg.2020.00194},
journal = {Frontiers in Energy Research},
number = ,
volume = 8,
place = {Switzerland},
year = {Thu Feb 04 00:00:00 EST 2021},
month = {Thu Feb 04 00:00:00 EST 2021}
}
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