Development and characterization of microsatellite loci for the endangered scrub lupine, Lupinus aridorum (Fabaceae)

Ricono, Angela; Bupp, Glen; Peterson, Cheryl; Nunziata, Schyler O.; Lance, Stacey L.; Pruett, Christin L.

doi:10.3732/apps.1500013

Title: Development and characterization of microsatellite loci for the endangered scrub lupine, Lupinus aridorum (Fabaceae)

Abstract

Microsatellite primers were developed in scrub lupine (Lupinus aridorum, Fabaceae), an endemic species to Florida that is listed as endangered in the United States, to assess connectivity among populations, identify hybrids, and examine genetic diversity. We isolated and characterized 12 microsatellite loci polymorphic in scrub lupine or in closely related species (i.e., sky-blue lupine [L. diffusus] and Gulf Coast lupine [L. westianus]). Loci showed low to moderate polymorphism, ranging from two to 14 alleles per locus and 0.01 to 0.86 observed heterozygosity. In conclusion, these loci are the first developed for Florida species of lupine and will be used to determine differentiation among species and to aid in conservation of the endangered scrub lupine.

Authors:: Ricono, Angela; Bupp, Glen; Peterson, Cheryl; Nunziata, Schyler O.; Lance, Stacey L.; Pruett, Christin L.

Publication Date:: Tue Apr 07 00:00:00 EDT 2015

Research Org.:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1787354

Alternate Identifier(s):: OSTI ID: 1345829; OSTI ID: 1787355

Grant/Contract Number:: DE‐FC09‐07SR22506; FC09-07SR22506

Resource Type:: Published Article

Journal Name:: Applications in Plant Science

Additional Journal Information:: Journal Name: Applications in Plant Science Journal Volume: 3 Journal Issue: 4; Journal ID: ISSN 2168-0450

Publisher:: Wiley Blackwell (John Wiley & Sons)

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Fabaceae; Florida; Lupinus; microsatellite; PCR primers

Citation Formats


                    Ricono, Angela, Bupp, Glen, Peterson, Cheryl, Nunziata, Schyler O., Lance, Stacey L., and Pruett, Christin L. Development and characterization of microsatellite loci for the endangered scrub lupine, Lupinus aridorum (Fabaceae).  United States: N. p., 2015. 
Web.  doi:10.3732/apps.1500013.

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                    Ricono, Angela, Bupp, Glen, Peterson, Cheryl, Nunziata, Schyler O., Lance, Stacey L., & Pruett, Christin L. Development and characterization of microsatellite loci for the endangered scrub lupine, Lupinus aridorum (Fabaceae).  United States.  https://doi.org/10.3732/apps.1500013

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                    Ricono, Angela, Bupp, Glen, Peterson, Cheryl, Nunziata, Schyler O., Lance, Stacey L., and Pruett, Christin L. Tue .  
"Development and characterization of microsatellite loci for the endangered scrub lupine, Lupinus aridorum (Fabaceae)".  United States.  https://doi.org/10.3732/apps.1500013.

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@article{osti_1787354,

  title        = {Development and characterization of microsatellite loci for the endangered scrub lupine, Lupinus aridorum (Fabaceae)},

  author       = {Ricono, Angela and Bupp, Glen and Peterson, Cheryl and Nunziata, Schyler O. and Lance, Stacey L. and Pruett, Christin L.},

  abstractNote = {Microsatellite primers were developed in scrub lupine (Lupinus aridorum, Fabaceae), an endemic species to Florida that is listed as endangered in the United States, to assess connectivity among populations, identify hybrids, and examine genetic diversity. We isolated and characterized 12 microsatellite loci polymorphic in scrub lupine or in closely related species (i.e., sky-blue lupine [L. diffusus] and Gulf Coast lupine [L. westianus]). Loci showed low to moderate polymorphism, ranging from two to 14 alleles per locus and 0.01 to 0.86 observed heterozygosity. In conclusion, these loci are the first developed for Florida species of lupine and will be used to determine differentiation among species and to aid in conservation of the endangered scrub lupine.},

  doi          = {10.3732/apps.1500013},

  journal      = {Applications in Plant Science},

  number       = 4,

  volume       = 3,

  place        = {United States},

  year         = {Tue Apr 07 00:00:00 EDT 2015},

  month        = {Tue Apr 07 00:00:00 EDT 2015}

}

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Works referenced in this record:

Site-specific genetic divergence in parallel hybrid zones suggests nonallopatric evolution of reproductive barriers: PARALLEL HYBRID ZONES IN
journal, August 2006

Panova, M.; Hollander, J.; Johannesson, K.
Molecular Ecology, Vol. 15, Issue 13
DOI: 10.1111/j.1365-294X.2006.03067.x

Development of microsatellite markers in Lupinus luteus (Fabaceae) and cross-species amplification in other lupine species
journal, August 2010

Gonzalez, Lorena B. Parra; Straub, Shannon C. K.; Doyle, Jeff J.
American Journal of Botany, Vol. 97, Issue 8
DOI: 10.3732/ajb.1000170

Rapid Microsatellite Identification from Illumina Paired-End Genomic Sequencing in Two Birds and a Snake
journal, February 2012

Castoe, Todd A.; Poole, Alexander W.; de Koning, A. P. Jason
PLoS ONE, Vol. 7, Issue 2
DOI: 10.1371/journal.pone.0030953

Isolation, phylogeny and evolution of the SymRK gene in the legume genus Lupinus L.
journal, July 2011

Mahé, Frédéric; Markova, Dragomira; Pasquet, Rémy
Molecular Phylogenetics and Evolution, Vol. 60, Issue 1
DOI: 10.1016/j.ympev.2011.04.017

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Title: Development and characterization of microsatellite loci for the endangered scrub lupine, Lupinus aridorum (Fabaceae)

Abstract

Citation Formats

Site-specific genetic divergence in parallel hybrid zones suggests nonallopatric evolution of reproductive barriers: PARALLEL HYBRID ZONES IN journal, August 2006

Development of microsatellite markers in Lupinus luteus (Fabaceae) and cross-species amplification in other lupine species journal, August 2010

Rapid Microsatellite Identification from Illumina Paired-End Genomic Sequencing in Two Birds and a Snake journal, February 2012

Isolation, phylogeny and evolution of the SymRK gene in the legume genus Lupinus L. journal, July 2011

Site-specific genetic divergence in parallel hybrid zones suggests nonallopatric evolution of reproductive barriers: PARALLEL HYBRID ZONES IN
journal, August 2006

Development of microsatellite markers in Lupinus luteus (Fabaceae) and cross-species amplification in other lupine species
journal, August 2010

Rapid Microsatellite Identification from Illumina Paired-End Genomic Sequencing in Two Birds and a Snake
journal, February 2012

Isolation, phylogeny and evolution of the SymRK gene in the legume genus Lupinus L.
journal, July 2011