DNA Reaction–Diffusion Attractor Patterns
[1];
[1];
[2]
- Department of Chemical and Biomolecular Engineering Johns Hopkins University 3400 N. Charles Street Baltimore MD 21218 USA
- Department of Chemical and Biomolecular Engineering Johns Hopkins University 3400 N. Charles Street Baltimore MD 21218 USA, Department of Computer Science Johns Hopkins University 3400 N. Charles Street Baltimore MD 21218 USA
Abstract Living systems can form and recover complex chemical patterns with precisely sized features in the ranges of tens or hundreds of microns. We show how designed reaction–diffusion processes can likewise produce precise patterns, termed attractor patterns, that reform their precise shape after being perturbed. We use oligonucleotide reaction networks, photolithography, and microfluidic delivery to form precisely controlled attractor patterns and study the responses of these patterns to different localized perturbations. Linear and “hill”‐shaped patterns formed and stabilized into shapes and at time scales consistent with reaction–diffusion models. When patterns were perturbed in particular locations with UV light, they reliably reformed their steady‐state profiles. Recovery also occurred after repeated perturbations. By designing the far‐from‐equilibrium dynamics of a chemical system, this study shows how it is possible to design spatial patterns of molecules that are sustained and regenerated by continually evolving towards a specific steady state configuration.
- Sponsoring Organization:
- USDOE
- Grant/Contract Number:
- SC0015906
- OSTI ID:
- 1786163
- Journal Information:
- Angewandte Chemie, Journal Name: Angewandte Chemie Journal Issue: 1 Vol. 133; ISSN 0044-8249
- Publisher:
- Wiley Blackwell (John Wiley & Sons)Copyright Statement
- Country of Publication:
- Germany
- Language:
- English
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